Anti-CD63抗体[MEM-259] (ab8219)

ab2215 should be used at a concentration of 2-4 ug/ml in blocking buffer when incubating your membrane whereas ab8216 should be used at a concentration of 2 ug/ml.

The gel should be run in non-reducing conditions as these antibodies do not recognise their antigens well when the western blot sample contains reducing agent. We therefore advise against adding beta-mercaptoethanol or DTT in the loading buffer.

We have not tested the stability of ab8219 under the alkaline conditions that you described; thus, we cannot guarantee that it will work under these conditions. The laboratory thinks it is possible that it could work, but only performing the experiment will tell.

We are not aware of a reference describing production and characterization of this CD63 antibody. The epitope the antibody recognizes on CD63 has not, to our knowledge, been mapped, but we do know that the epitope is extracellular when CD63 is integrated into the plasma membrane.

Thank you for contacting us.

The antibodies have not been tested for reactivity with bovine samples, and we have not received reports of bovine reactivity from our customers.

Please do not hesitate to contact us if you need any more advice or information.

Thank you for your inquiry.

Ab8219 (anti-CD63) is supplied as 100ul at a concentration of 1mg/ml. We have a customer who reported successfully using the antibody at a 1/200 dilution (0.2 mg/ml), but it may be necessary to optimize this for your own experiments. Please note that we recommend using non-reducing conditions for the western blot (i.e. no BME or DTT).

I hope this information helps. Please do not hesitate to contact us if you need anything further. Have a wonderful day, and best of luck in your research!