Anti-CD63抗体[MEM-259] (ab8219)
Key features and details
- Mouse monoclonal [MEM-259] to CD63
- Suitable for: Flow Cyt, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-CD63抗体[MEM-259]
参阅全部 CD63 一抗 -
描述
小鼠单克隆抗体[MEM-259] to CD63 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, ICC, IHC-Pmore details
不适用于: WB -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD63. HPB-ALL T cell line
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阳性对照
- IHC: Human spleen sections; ICC: Human HeLa cells, primary fibroblasts and skin fibroblasts tissues; Flow cyt: Human blood samples.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
MEM-259 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
应用 | Ab评论 | 说明 |
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Flow Cyt | (2) |
Use a concentration of 2 µg/ml.
Use at an assay dependant concentration. For detection of activated platelets, neutrophils and basophils. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC | (1) |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use a concentration of 10 µg/ml.
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说明 |
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Flow Cyt
Use a concentration of 2 µg/ml. Use at an assay dependant concentration. For detection of activated platelets, neutrophils and basophils. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 10 µg/ml. |
靶标
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功能
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
组织特异性
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
序列相似性
Belongs to the tetraspanin (TM4SF) family. -
细胞定位
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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数据库链接
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
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别名
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [MEM-259] (ab8219)
Immunohistochemistry staining of human spleen (paraffin sections) using ab8219.
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Immunocytochemistry staining of human skin fibroblasts with ab8219 (green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).
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Flow Cytometry - Anti-CD63 antibody [MEM-259] (ab8219)This image is courtesy of an anonymous Abreviewab8219 staining CD63 in human Fibrosarcoma HT1080 cell line by flow cytometry. Cells were incubated with primary antibody for 1 hour. ab7003, a donkey polyclonal to mouse Ig, was used as the secondary antibody.
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Immunocytochemistry staining of CD63 in human HeLa cell line using ab8219 (green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
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Flow cytometry analysis staining CD63 in IgE- activated peripheral blood using ab8219 (2μg/ml).
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Immunocytochemistry staining of CD63 in human primary fibroblasts using ab8219 (green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (94)
ab8219 被引用在 94 文献中.
- Park SJ et al. Vinexin contributes to autophagic decline in brain ageing across species. Cell Death Differ 29:1055-1070 (2022). PubMed: 34848853
- Krishn SR et al. Small extracellular vesicle-mediated ITGB6 siRNA delivery downregulates the αVβ6 integrin and inhibits adhesion and migration of recipient prostate cancer cells. Cancer Biol Ther 23:173-185 (2022). PubMed: 35188070
- Governa V et al. Landscape of surfaceome and endocytome in human glioma is divergent and depends on cellular spatial organization. Proc Natl Acad Sci U S A 119:N/A (2022). PubMed: 35217608
- Solvik TA et al. Secretory autophagy maintains proteostasis upon lysosome inhibition. J Cell Biol 221:N/A (2022). PubMed: 35446347
- Park SS et al. Temsirolimus Enhances Anti-Cancer Immunity by Inducing Autophagy-Mediated Degradation of the Secretion of Small Extracellular Vesicle PD-L1. Cancers (Basel) 14:N/A (2022). PubMed: 36077620