重组Anti-CD63抗体[EPR5702] - Late Endosome Marker (ab134045)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5702] to CD63 - Late Endosome Marker
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD63抗体[EPR5702] - Late Endosome Marker
参阅全部 CD63 一抗 -
描述
兔单克隆抗体[EPR5702] to CD63 - Late Endosome Marker -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human platelet, HL-60 and A375 cell lysates and human melanoma tissue lysate. Brefeldin A treated wild-type HAP1 whole cell lysate. IHC-P: Human melanoma and tonsil tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5702 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134045于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 30-65 kDa (predicted molecular weight: 26 kDa).
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IHC-P | (1) |
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 30-65 kDa (predicted molecular weight: 26 kDa). |
IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
靶标
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功能
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
组织特异性
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
序列相似性
Belongs to the tetraspanin (TM4SF) family. -
细胞定位
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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数据库链接
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
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别名
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
图片
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All lanes : Anti-CD63 antibody [EPR5702] - Late Endosome Marker (ab134045) at 1/1000 dilution
Lane 1 : Wild-type HepG2 Vehicle Control Brefeldin A (0 u/mL, 24h) cell lysate
Lane 2 : Wild-type HepG2 Treated Brefeldin A (10 u/mL, 24h) cell lysate
Lane 3 : CD63 knockout HepG2 Vehicle Control Brefeldin A (0 u/mL, 24h) cell lysate
Lane 4 : CD63 knockout HepG2 Treated Brefeldin A (10 u/mL, 24h) cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Anti-CD63 antibody [EPR5702] (ab134045) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab134045 was shown to bind specifically to CD63. A band was observed at 30 kDa in wild-type HepG2 cell lysates with no signal observed at this size in CD63 knockout cell line ab280796 (knockout cell lysate ab283828). To generate this image, wild-type and CD63 knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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All lanes : Anti-CD63 antibody [EPR5702] - Late Endosome Marker (ab134045) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : Brefeldin A treated wild-type HAP1 whole cell lysate
Lane 3 : CD63 knockout HAP1 whole cell lysate
Lane 4 : Brefeldin A treated CD63 knockout HAP1 whole cell lysate
Lane 5 : HL60 whole cell lysate
Lane 6 : Human platelets whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 26 kDaLanes 1 - 6: Merged signal (red and green). Green - ab134045 observed at 26 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab134045 was shown to specifically react with CD63 in wild-type HAP1 Brefeldin A treated cells as signal was lost in HAP1 Brefeldin A treated CD63 knockout cells. Wild-type and CD63 knockout samples were subjected to SDS-PAGE. Ab134045 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] - Late Endosome Marker (ab134045)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD63 with purified ab134045 at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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All lanes : Anti-CD63 antibody [EPR5702] - Late Endosome Marker (ab134045) at 1/5000 dilution (purified)
Lane 1 : A375 whole cell lysate
Lane 2 : HL-60 whole cell lysate
Lane 3 : Human melanoma tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 30-65 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] - Late Endosome Marker (ab134045)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human melanoma tissue labelling CD63 with unpurified ab134045 at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] - Late Endosome Marker (ab134045)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue (negative control) labelling CD63 with purified ab134045 at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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All lanes : Anti-CD63 antibody [EPR5702] - Late Endosome Marker (ab134045) at 1/1000 dilution (unpurified)
Lane 1 : Human platelet lysate
Lane 2 : Human melanoma lysate
Lane 3 : HL-60 lysate
Lane 4 : A375 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 26 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (379)
ab134045 被引用在 379 文献中.
- Zhang J et al. Protective effect of small extracellular vesicles (EVs) derived from ACE2-modified human umbilical cord mesenchymal stem cells against renal ischemia-reperfusion injury. Nephrology (Carlton) 29:5-17 (2024). PubMed: 37667547
- Du Y et al. Exosome-mediated long noncoding RNA (lncRNA) PART1 suppresses malignant progression of oral squamous cell carcinoma via miR-17-5p/SOCS6 axis. Turk J Med Sci 53:630-639 (2023). PubMed: 37476905
- Hanna N et al. Biodistribution of mRNA COVID-19 vaccines in human breast milk. EBioMedicine 96:104800 (2023). PubMed: 37734205
- Lv Q et al. Exosomal miR-146a-5p derived from human umbilical cord mesenchymal stem cells can alleviate antiphospholipid antibody-induced trophoblast injury and placental dysfunction by regulating the TRAF6/NF-κB axis. J Nanobiotechnology 21:419 (2023). PubMed: 37957714
- Luo H et al. Large-scale Isolation of Exosomes Derived from NK Cells for Anti-tumor Therapy. Bio Protoc 13:e4693 (2023). PubMed: 37342158