Anti-CD62P抗体[Psel.KO.2.7] (ab54427)
![Immunohistochemistry (Frozen sections) - Anti-CD62P antibody [Psel.KO.2.7] (ab54427)](https://www.abcam.cn/ps/products/54/ab54427/Images/ab54427-367827-anticd62ppselko27ihcfrhutonsil.jpg "Immunohistochemistry (Frozen sections) - Anti-CD62P antibody [Psel.KO.2.7] (ab54427)")
Key features and details
- Mouse monoclonal [Psel.KO.2.7] to CD62P
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-CD62P抗体[Psel.KO.2.7]
参阅全部 CD62P 一抗 -
描述
小鼠单克隆抗体[Psel.KO.2.7] to CD62P -
宿主
Mouse -
经测试应用
适用于: IHC-Fr, Flow Cytmore details -
种属反应性
与反应: Human
预测可用于: Sheep
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免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt: Human whole blood; IHC-Fr: Human tonsil tissue.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant -
克隆
单克隆 -
克隆编号
Psel.KO.2.7 -
骨髓瘤
NS1 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab54427于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
Use a concentration of 5 µg/ml.
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| Flow Cyt |
Use a concentration of 0.2 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
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IHC-Fr
Use a concentration of 5 µg/ml. |
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Flow Cyt
Use a concentration of 0.2 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. -
组织特异性
Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. -
疾病相关
Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
序列相似性
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 9 Sushi (CCP/SCR) domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6403 Human
- Omim: 173610 Human
- SwissProt: P16109 Human
- Unigene: 73800 Human
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别名
- Antigen CD62 antibody
- CD62 antibody
- CD62 antigen-like family member P antibody
see all
图片
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Immunohistochemistry (Frozen sections) - Anti-CD62P antibody [Psel.KO.2.7] (ab54427)
IHC image of CD62P staining in a section of frozen normal human tonsil*. The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab54427 at 5µg/ml. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488), 1/1000)) (shown in green) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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![Flow Cytometry - Anti-CD62P antibody [Psel.KO.2.7] (ab54427)](https://www.abcam.cn/ps/products/54/ab54427/Images/ab54427-412843-anti-cd62p-antibody-psel-ko-2-7-flow-cytometry-whole-blood-cells-human.jpg)
Flow Cytometry - Anti-CD62P antibody [Psel.KO.2.7] (ab54427)Flow cytometry staining of human whole blood with ab54427 (right) or mouse IgG1κ (ab170190) isotype (left). Red blood cells of 200 µL blood were lysed, then cells were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10% normal goat serum to block Fc receptors and non-specific protein-protein interaction followed by the antibody (ab54427) or mouse IgG1κ (ab170190) isotype (1x106 in 100 µL at 0.2 µg/mL) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice.
The cells were simultaneously stained with CD42a PE.
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on alive neutrophils.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab54427 被引用在 7 文献中.
- Liang C et al. Platelets Stimulate Liver Regeneration in a Rat Model of Partial Liver Transplantation. Liver Transpl 27:719-734 (2021). PubMed: 33277780
- Shen DS et al. Aspirin eugenol ester inhibits agonist-induced platelet aggregation in vitro by regulating PI3K/Akt, MAPK and Sirt 1/CD40L pathways. Eur J Pharmacol 852:1-13 (2019). PubMed: 30797789
- Su C et al. Si-Miao-Yong-An Decoction Protects Against Cardiac Hypertrophy and Dysfunction by Inhibiting Platelet Aggregation and Activation. Front Pharmacol 10:990 (2019). PubMed: 31619988
- Li XH et al. Effect of Platelet-derived P-selectin on Neutrophil Recruitment in a Mouse Model of Sepsis-induced Acute Kidney Injury. Chin Med J (Engl) 130:1694-1699 (2017). Mouse . PubMed: 28685720
- Holloway PM et al. Sulforaphane induces neurovascular protection against a systemic inflammatory challenge via both Nrf2-dependent and independent pathways. Vascul Pharmacol 85:29-38 (2016). PubMed: 27401964
- Baron DM et al. Adverse effects of hemorrhagic shock resuscitation with stored blood are ameliorated by inhaled nitric oxide in lambs*. Crit Care Med 41:2492-501 (2013). Sheep . PubMed: 23887236
- Massaguer A et al. Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P). Tissue Antigens 56:117-28 (2000). IHC-Fr, Flow Cyt ; Mouse, Rat, Human . PubMed: 11019911