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AB119834

Anti-CD62L 抗体 [MEL-14]

Anti-CD62L antibody [MEL-14]

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(8 Publications)

Rat Recombinant Monoclonal CD62L antibody. Suitable for ICC/IF, Flow Cyt and reacts with Mouse samples. Cited in 8 publications.

查看别名

CD62L, Lnhr, Ly-22, Ly22, Sell, L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, Lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen, LAM-1, LECAM1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD62L antibody [MEL-14] (AB119834)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD62L antibody [MEL-14] (AB119834)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse spleen cells labelling CD62L with ab119834 at 1/100 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in some subset of mouse spleen cells is observed. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab119834 at 1/100 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

-ve control 2 : ab179513 at 1/200 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Flow Cytometry - Anti-CD62L antibody [MEL-14] (AB119834)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD62L antibody [MEL-14] (AB119834)

Flow cytometric analysis of Mouse splenocyte cells labelling CD62L with ab119834 at 1/500 dilution (0.2 ug). Goat anti rat IgG (Alexa Fluor® 488, ab150157) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rat IgG (Left) or ab119834 (Right). Then stained with anti-CD4 conjugated to Alexa Fluor®647 and anti-CD3 conjugated to PerCP.
Gated on viable CD3+ T cells.

Flow Cytometry - Anti-CD62L antibody [MEL-14] (AB119834)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD62L antibody [MEL-14] (AB119834)

Flow cytometric analysis of Mouse splenocyte cells labelling CD62L with ab119834 at 1/500 dilution (0.2 ug). Goat anti rat IgG (Alexa Fluor® 488, ab150157) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rat IgG (Left) or ab119834 (Right). Then stained with anti-CD4 conjugated to Alexa Fluor®647.Gated on viable lymphocytes.

不同偶联物与剂型 (6)

  • redFluor™ 710

    redFluor™ 710 Anti-CD62L antibody [MEL-14]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD62L antibody [MEL-14]

  • Biotin

    Biotin Anti-CD62L antibody [MEL-14]

  • 519 FITC

    FITC Anti-CD62L antibody [MEL-14]

  • 660 APC

    APC Anti-CD62L antibody [MEL-14]

  • Carrier free

    Anti-CD62L antibody [MEL-14] - BSA and Azide free

关键信息

宿主种属

Rat

克隆

Monoclonal

克隆号

MEL-14

亚型

IgG2a

不含载体蛋白

No

反应种属

Mouse

应用

ICC/IF, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

ab119834 reacts with mouse CD62L, a 75 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/1000", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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AB199085

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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Ion exchange chromatography
存储溶液
pH: 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD62L also known as L-selectin is a cell adhesion molecule with a mass of approximately 74 kDa. It is part of the L-selectin family and is found on the surface of leukocytes including T cells B cells neutrophils and monocytes. Mouse models frequently study CD62L under the name "MEL-14". CD62L plays an important role in mediating the adhesion and rolling of leukocytes on blood vessel endothelial cells during the immune response. Its structure allows it to interact specifically with carbohydrates on endothelial cells facilitating the initial steps of leukocyte extravasation into tissues.
Biological function summary

CD62L is involved in the migration of leukocytes into lymphoid tissues and sites of inflammation. It is not known to be part of a larger protein complex; rather it functions independently on the cell membrane. CD62L also assists in the homing of naive lymphocytes to peripheral lymph nodes. The molecule regulates how immune cells enter tissues through high endothelial venules acting as a critical entry point for leukocyte trafficking.

Pathways

CD62L contributes significantly to the immune trafficking pathway and leukocyte adhesion cascade. It works closely with other adhesion molecules like CD44 and CD54 (ICAM-1) which are important within the context of cellular responses during inflammation. CD62L's function in these pathways ensures that immune cells are properly directed to areas where they are needed most whether that is secondary lymphoid organs or inflamed tissues.

CD62L expression and function have been associated with inflammatory conditions and certain immune-related pathologies such as multiple sclerosis and rheumatoid arthritis. Altered CD62L expression can affect lymphocyte homing potentially playing a role in the pathogenesis of these conditions. The role of CD62L is also linked to proteins like CD44 which can modulate cell interactions and have overlapping functions in disease mechanisms influencing disease progression or resolution.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes (PubMed : 1693096). Promotes initial tethering and rolling of leukocytes in endothelia (By similarity).
See full target information Sell
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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Nature immunology 23:518-531 PubMed35354953

2022

Neutrophils direct preexisting matrix to initiate repair in damaged tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Adrian Fischer,Juliane Wannemacher,Simon Christ,Tim Koopmans,Safwen Kadri,Jiakuan Zhao,Mahesh Gouda,Haifeng Ye,Martin Mück-Häusl,Peter W Krenn,Hans-Günther Machens,Reinhard Fässler,Philipp-Alexander Neumann,Stefanie M Hauck,Yuval Rinkevich

Biomaterials 242:119932 PubMed32169772

2020

Nanosponges of circulating tumor-derived exosomes for breast cancer metastasis inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Ye,Kaiyuan Wang,Qi Lu,Jian Zhao,Menglin Wang,Qiming Kan,Haotian Zhang,Yongjun Wang,Zhonggui He,Jin Sun

eLife 3: PubMed25347065

2014

CD28 expression is required after T cell priming for helper T cell responses and protective immunity to infection.

Applications

Unspecified application

Species

Unspecified reactive species

Michelle A Linterman,Alice E Denton,Devina P Divekar,Ilona Zvetkova,Leanne Kane,Cristina Ferreira,Marc Veldhoen,Simon Clare,Gordon Dougan,Marion Espéli,Kenneth G C Smith

Journal of immunology (Baltimore, Md. : 1950) 177:5-9 PubMed16785489

2006

A cell-surface molecule involved in organ-specific homing of lymphocytes. 1983.

Applications

Unspecified application

Species

Unspecified reactive species

W Michael Gallatin,Irving L Weissman,Eugene C Butcher

Blood 103:1534-41 PubMed14551132

2003

Transfer of allogeneic CD62L- memory T cells without graft-versus-host disease.

Applications

Unspecified application

Species

Unspecified reactive species

Benny J Chen,Xiuyu Cui,Gregory D Sempowski,Congxiao Liu,Nelson J Chao

Journal of immunology (Baltimore, Md. : 1950) 168:2659-66 PubMed11884430

2002

L-selectin is not required for T cell-mediated autoimmune diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Randall H Friedline,Carmen P Wong,Douglas A Steeber,Thomas F Tedder,Roland Tisch

Infection and immunity 70:27-35 PubMed11748160

2001

Interleukin-2 and loss of immunity in experimental Mycobacterium avium infection.

Applications

Unspecified application

Species

Unspecified reactive species

Stuart I Mannering,Christina Cheers

Journal of virology 73:9161-9 PubMed10516023

1999

Hierarchal utilization of different T-cell receptor Vbeta gene segments in the CD8(+)-T-cell response to an immunodominant Moloney leukemia virus-encoded epitope in vivo.

Applications

Flow Cyt

Species

Mouse

P Brawand,J C Cerottini,H R MacDonald
View all publications
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