为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Immunology Cell Type Markers CD Adhesion

Anti-CD62E抗体[UZ6] (ab2497)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (4)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunocytochemistry/ Immunofluorescence - Anti-CD62E antibody [UZ6] (ab2497)

    Key features and details

    • Rat monoclonal [UZ6] to CD62E
    • Suitable for: ICC/IF
    • Reacts with: Mouse
    • Isotype: IgG2b

    你可能会感兴趣

    蛋白
    Product image
    Recombinant Human CD62E protein (ab167744)
    偶联
    Product image
    Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)
    一抗
    Product image
    FITC Anti-Kappa light chain antibody (ab4208)

    查看更多相关产品

    概述

    • 产品名称

      Anti-CD62E抗体[UZ6]
      参阅全部 CD62E 一抗
    • 描述

      大鼠单克隆抗体[UZ6] to CD62E
    • 宿主

      Rat
    • 经测试应用

      适用于: ICC/IFmore details
    • 种属反应性

      与反应: Mouse
    • 阳性对照

      • ICC/IF: CHO-cells transfected with E-Selectin.
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 纯度

      Protein A purified
    • 克隆

      单克隆
    • 克隆编号

      UZ6
    • 骨髓瘤

      Sp2/0
    • 同种型

      IgG2b
    • 研究领域

      • Immunology
      • Cell Type Markers
      • CD
      • Adhesion
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Leukocyte recruitment
      • Cell adhesion molecules
      • Stem Cells
      • Endothelial Progenitors
      • Endothelial Markers
      • Cardiovascular
      • Cardiovascular Markers
      • Cell Markers
      • Endothelial Cells
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control (ab18541)
    • Recombinant Protein

      • Recombinant Human CD62E protein (ab167744)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab2497于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC/IF
    Use at an assay dependent concentration.
    说明
    ICC/IF
    Use at an assay dependent concentration.

    靶标

    • 功能

      Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
    • 序列相似性

      Belongs to the selectin/LECAM family.
      Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 6 Sushi (CCP/SCR) domains.
    • 细胞定位

      Membrane.
    • Target information above from: UniProt accession P16581 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 20339 Mouse
      • SwissProt: Q00690 Mouse
      • Unigene: 5245 Mouse
      • 别名

        • CD26E antibody
        • CD62 antigen-like family member E antibody
        • CD62E antibody
        • E selectin antibody
        • E-selectin antibody
        • ELAM antibody
        • ELAM-1 antibody
        • ELAM1 antibody
        • Endothelial adhesion molecule 1 antibody
        • Endothelial leukocyte adhesion molecule 1 antibody
        • ESEL antibody
        • LECAM2 antibody
        • Leukocyte-endothelial cell adhesion molecule 2 antibody
        • LYAM2_HUMAN antibody
        • Sele antibody
        • Selectin E antibody
        see all

      图片

      • Immunocytochemistry/ Immunofluorescence - Anti-CD62E antibody [UZ6] (ab2497)
        Immunocytochemistry/ Immunofluorescence - Anti-CD62E antibody [UZ6] (ab2497)

        CHO-cells transfected with E-Selectin stained wtih ab2497 at 1/500. 

      实验方案

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (4)

      发表研究结果有使用 ab2497?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab2497 被引用在 4 文献中.

      • Cao H  et al. The protective effect of vitexinin septic encephalopathy by reducing leukocyte-endothelial adhesion and inflammatory response. Ann Palliat Med 9:2079-2089 (2020). PubMed: 32692224
      • Meng ZD  et al. Effects of RNA interference-mediated E-selectin gene silencing on cell adhesion molecule expression and cell-cell adhesion in vascular endothelial cells in mice with immunologic contact urticaria. J Cell Biochem 120:115-125 (2019). PubMed: 30206961
      • Sapieha P  et al. 5-Lipoxygenase metabolite 4-HDHA is a mediator of the antiangiogenic effect of ?-3 polyunsaturated fatty acids. Sci Transl Med 3:69ra12 (2011). PubMed: 21307302
      • Hammel M  et al. Species-specific and conserved epitopes on mouse and human E-selectin important for leukocyte adhesion. Exp Cell Res 269:266-74 (2001). PubMed: 11570819

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-4 of 4 Abreviews or Q&A

      Immunohistochemistry (Frozen sections) abreview for Anti-CD62E antibody [UZ6]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Brain)
      Specification
      Brain
      Fixative
      Paraformaldehyde
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Dr. Thomas Weissensteiner

      Verified customer

      提交于 Oct 22 2009

      Question

      Thank you so much for your email. I would like to ask you a question.

      I have to target 3 proteins in my Mouse Brain Enothelial cell line (bEnd.3), which are E-selectin (CD62E), VCAM and ICAM.

      I have chosen ab2497 for E-selectin , ab61993 for VCAM and ab119871 for ICAM, I would like to know whether these antibodies are going to target the whole length of my molecules, or just bind to specific site of the protein?

      Because I am not sure after my treatment, any of these proteins will be externalised or they will stay in the cytoplasm.

      Read More

      Abcam community

      Verified customer

      Asked on Apr 27 2012

      Answer

      Thanks you for your email.
      In my previous email there is a correction about the expiry date. The expiry date should be read as 25-08-2012.
      I have asked lab to release the immunogen information of these antibodies; I will be able to send the answer to your question soon.
      Many thanks for having patience!

      Read More

      Abcam Scientific Support

      回复于 Apr 27 2012

      Question

      Hi Technical Support, how are you today?

      Could you please advise me on the difference between the corresponding pairs below:

      ab 22631 Anti-CD62E antibody

      &

      ab2497 Anti-CD62E antibody

      and

      ab65472 Quick cell Proliferation assay kit

      &

      ab65474 Quick cell Proliferation assay kit II

      thanks very much for your help!

      In the meantime, have a great day! J

      Kind regards,

      Read More

      Abcam community

      Verified customer

      Asked on Apr 26 2012

      Answer

      Thank you very much for your email!

      I hope you are well as well! Here we are fine - we have one of our first days of spring :-)

      In regards to your questions:

      The CD62E antibodies ab22631 et ab2497 are two different clones. Indeed, both are monoclonal rat antibodies, and I would think from the same fusion/productions, however they are two distinct antibodies - once clone UY7 and once clone UZ6. as therefore the epitope is most likely also different, they need therefore to be tested separately for all applications and species reactivity. Please let me know if you would need any additional information.

      For the quick cell proliferation kits, the difference is that the ab65474 contains the stop solution as well, and the ab65472 not.

      I hope this informationis helpful. Please do not hesitate to contact us again should you have any additional questions.

      I wish you a great day as well :-)

      Read More

      Abcam Scientific Support

      回复于 Apr 26 2012

      Question

      1) Crossreactivities? 2) Is it function blocking or not?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 29 2003

      Answer

      1) Crossreactivities: this antibody reacts with mouse E-selectin but not with human E-selectin. Other species not tested. 2)Blocking function: Ab2497 (20 microgram/ml) inhibited up to 70% of mouse PMN and up to 95% of human peripheral blood mononuclear cells adhesion to LPS-activated mlEND1 cells. For experimental conditions and figure see Hammel et al., Exp Cell Res 269: 266 (2001).

      Read More

      Abcam Scientific Support

      回复于 Feb 03 2003

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      通过邮件收到学术资源和促销信息 注册
      研究类别(A-Z)
      • 肿瘤研究
      • 心血管研究
      • 细胞生物学
      • 发育生物学
      • 染色质及细胞核信号转导研究
      • 免疫学
      • 代谢
      • 微生物学
      • 神经生物学
      • 信号转导研究
      • 干细胞研究
      产品种类(A-Z)
      • 一抗
      • 二抗
      • 生化试剂
      • 亚类对照
      • 流式细胞仪多色选择器
      • 试剂盒
      • 内参对照
      • 细胞裂解液
      • 多肽
      • 蛋白酶
      • 组织切片
      • 蛋白标签及细胞标记物
      • 相关试剂及工具
      帮助与支持
      • 技术支持
      • 技术FAQ
      • 实验步骤与建议
      • 购买FAQ
      • RabMAb 产品
      • 培训课程
      • 按靶标浏览
      企业
      • 企业资讯
      • 投资者关系
      • 公司新闻
      • 招贤纳士
      • 关于我们
      活动通知
      • 商品展览
      • 会议
      全球网站
      • abcam.com
      • abcam.co.jp
      QR code for Weechat

      微信扫码 关注我们

      沪ICP备14046373号-1

      沪公网安备 31011502007413号

      • 销售条款
      • 网站使用条款
      • Cookie政策
      • 隐私权政策
      • 法律
      © 1998-2023 艾博抗(上海)贸易有限公司版权所有