Anti-CD59抗体[MEM-43] (ab9182)
Key features and details
- Mouse monoclonal [MEM-43] to CD59
- Suitable for: IP, IHC-Fr, IHC, ICC/IF, Flow Cyt, WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
Anti-CD59抗体[MEM-43]
参阅全部 CD59 一抗 -
描述
小鼠单克隆抗体[MEM-43] to CD59 -
宿主
Mouse -
特异性
CD59 antigen (human). MEM-43 identified CD59 as the new cluster on 4th HLDA Workshop. MEM-43 reacts with well defined epitope (W40, R53).
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经测试应用
适用于: IP, IHC-Fr, IHC, ICC/IF, Flow Cyt, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD59. Thymocytes and T lymphocytes
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阳性对照
- ICC/IF: Human fibrosarcoma cells. Flow Cyt: HT1080 cells. IHC: human placenta tissue
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常规说明
When originally tested in WB, SDS was included in the sample buffer, however, feedback from one researcher has shown that it is best to omit SDS from the sample buffer.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Do Not Freeze. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
MEM-43 -
骨髓瘤
unknown -
同种型
IgG2a -
轻链类型
unknown -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IHC |
Use a concentration of 10 µg/ml.
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ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 17911601
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Flow Cyt | (1) |
Use a concentration of 0.5 - 4 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB |
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.
Use under non-reducing conditions. |
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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IP
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC
Use a concentration of 10 µg/ml. |
ICC/IF
Use at an assay dependent concentration. PubMed: 17911601 |
Flow Cyt
Use a concentration of 0.5 - 4 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. Use under non-reducing conditions. |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. -
疾病相关
Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300]. -
序列相似性
Contains 1 UPAR/Ly6 domain. -
翻译后修饰
N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. -
细胞定位
Cell membrane. Secreted. Soluble form found in a number of tissues. - Information by UniProt
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数据库链接
- Entrez Gene: 966 Human
- Omim: 107271 Human
- SwissProt: P13987 Human
- Unigene: 278573 Human
- Unigene: 709466 Human
- Unigene: 710641 Human
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别名
- 16.3A5 antibody
- 1F5 antibody
- 1F5 antigen antibody
see all
图片
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Flow cytometric analysis of Human Peripheral Blood cells labelling CD59 with ab9182 at 0.3 ug/ml showing separation of human neutrophil granulocytes (red-filled) from human CD59 negative blood debris (black-dashed).
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Immunohistochemistry parafin embedded sections staining of huam palacenta tissue using ab9182 with a concentration of 10µg / ml
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (24)
ab9182 被引用在 24 文献中.
- Ma D et al. Kidney transplantation from triple-knockout pigs expressing multiple human proteins in cynomolgus macaques. Am J Transplant 22:46-57 (2022). PubMed: 34331749
- Stadlbauer K et al. Bispecific mAb2 Antibodies Targeting CD59 Enhance the Complement-Dependent Cytotoxicity Mediated by Rituximab. Int J Mol Sci 23:N/A (2022). PubMed: 35563599
- Brandel A et al. The Gb3-enriched CD59/flotillin plasma membrane domain regulates host cell invasion by Pseudomonas aeruginosa. Cell Mol Life Sci 78:3637-3656 (2021). PubMed: 33555391
- Yan B et al. Effects of the Bone/Bone Marrow Microenvironments on Prostate Cancer Cells and CD59 Expression. Biomed Res Int 2020:2753414 (2020). PubMed: 32337233
- Kalappurakkal JM et al. Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading. Cell 177:1738-1756.e23 (2019). PubMed: 31104842