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AB9182

Anti-CD59 抗体 [MEM-43]

Anti-CD59 antibody [MEM-43]

4

(2 Reviews)

|

(25 Publications)

Anti-CD59 antibody [MEM-43] (ab9182) is a mouse monoclonal antibody detecting CD59 in Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF. Suitable for Human.

- Over 20 publications
- Trusted since 2002

查看别名

CD59, MIC11, MIN1, MIN2, MIN3, MSK21, CD59 glycoprotein, 1F5 antigen, 20 kDa homologous restriction factor, MAC-inhibitory protein, MEM43 antigen, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin, HRF-20, HRF20, MAC-IP, MACIF, MIRL

2 Images
Flow Cytometry - Anti-CD59 antibody [MEM-43] (AB9182)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD59 antibody [MEM-43] (AB9182)

Flow cytometric analysis of Human Peripheral Blood cells labelling CD59 with ab9182 at 0.3 ug/ml showing separation of human neutrophil granulocytes (red-filled) from human CD59 negative blood debris (black-dashed).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43] (AB9182)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43] (AB9182)

Immunohistochemistry parafin embedded sections staining of huam palacenta tissue using ab9182 with a concentration of 10μg / ml

不同偶联物与剂型 (5)

  • Biotin

    Biotin Anti-CD59 antibody [MEM-43]

  • 660 APC

    APC Anti-CD59 antibody [MEM-43]

  • 578 PE

    PE Anti-CD59 antibody [MEM-43]

  • 519 FITC

    FITC Anti-CD59 antibody [MEM-43]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD59 antibody [MEM-43]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

MEM-43

亚型

IgG2a

轻链类型

kappa

不含载体蛋白

No

反应种属

Human

应用

Flow Cyt, ICC/IF, IP, IHC, IHC-P, IHC-Fr, WB

applications

免疫原

Cell preparation containing CD59 protein. The exact immunogen used to generate this antibody is proprietary information.

P13987

特异性

CD59 antigen (human). MEM-43 identified CD59 as the new cluster on 4th HLDA Workshop. MEM-43 reacts with well defined epitope (W40, R53).

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHC-species-checked": "guaranteed", "IHC-species-dilution-info": "1/10", "IHC-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5-4 µg/mL", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Use under non-reducing conditions.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "10 µg/mL", "IHCFr-species-notes": "<p></p>" } } }

产品详情

What is this antibody validated in?
Anti-CD59 antibody [MEM-43] (ab9182) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of CD59?
Anti-CD59 [MEM-43] (ab9182) specifically detects a band for CD59 (UniProt: P13987) at a molecular weight of 14kDa.

Trusted by the scientific community
Anti-CD59 [MEM-43] (ab9182) was first used in a scientific publication in 2002 and has been cited over 20 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [MEM-43] also available for your convenience: ab9182, FITC - ab18237, Biotin - ab28114, PE - ab36421, APC - ab36467, Alexa Fluor® 647 - ab187769

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
纯化说明
Purity >95% by SDS-PAGE.
存储溶液
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Do Not Freeze

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD59 also known as protectin is a protein with a molecular mass of approximately 18-25 kDa. It is widely expressed on the surface of many human cells including red blood cells and various types of leukocytes. CD59 serves as an inhibitor of the complement membrane attack complex (MAC) preventing cell lysis caused by terminal complement proteins. Through this mechanism it regulates complement activation and maintains cell integrity by halting the formation of MAC.
Biological function summary

One important feature of CD59 is its role as a glycosylphosphatidylinositol (GPI)-anchored protein involved in safeguarding cells from complement-mediated damage. It does not exist within a larger complex but functions at the cell surface to inhibit the assembly of complement components C5b-9 which form the MAC. This ability to inhibit MAC is essential in maintaining self-tissue from unintended damage during immune responses.

Pathways

CD59 participates in the regulation of the complement cascade specifically within the terminal pathway. The complement system serves as a bridge between innate and adaptive immunity contributing to processes like opsonization and cell lysis. CD59's inhibitory action directly impacts pathways that utilize terminal components such as C5b. Effective CD59 function prevents excessive complement activation ensuring that an immune response does not damage host tissues. It links closely with other complement regulatory proteins like CD55 which also mitigate complement cascade activation.

Deficiencies or malfunctions in CD59 have notable implications. Paroxysmal nocturnal hemoglobinuria (PNH) is a disorder where lack of CD59 expression on blood cells leads to their increased destruction due to unregulated complement activity. Furthermore CD59's dysfunction or inadequacy also associates with atypical hemolytic uremic syndrome (aHUS) which involves mutant complement regulatory proteins causing overactivation of the complement system. These associations highlight the importance of CD59 in both maintaining cellular health and preventing pathophysiological conditions related to complement overactivity.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Potent inhibitor of the complement membrane attack complex (MAC) action, which protects human cells from damage during complement activation (PubMed : 11882685, PubMed : 1698710, PubMed : 2475111, PubMed : 2475570, PubMed : 2606909, PubMed : 9053451). Acts by binding to the beta-haipins of C8 (C8A and C8B) components of the assembling MAC, forming an intermolecular beta-sheet that prevents incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore (PubMed : 11882685, PubMed : 1698710, PubMed : 36797260).. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit complement membrane attack complex (MAC) assembly on cell membranes.
See full target information CD59

文献 (25)

Recent publications for all applications. Explore the full list and refine your search

Brain sciences 14: PubMed38928601

2024

CD59 Protects Primary Human Cerebrovascular Smooth Muscle Cells from Cytolytic Membrane Attack Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Carson D Whinnery,Ying Nie,Danilo S Boskovic,Salvador Soriano,Wolff M Kirsch

International journal of molecular sciences 23: PubMed35563599

2022

Bispecific mAb Antibodies Targeting CD59 Enhance the Complement-Dependent Cytotoxicity Mediated by Rituximab.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Stadlbauer,Peter Andorfer,Gerhard Stadlmayr,Florian Rüker,Gordana Wozniak-Knopp

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 22:46-57 PubMed34331749

2021

Kidney transplantation from triple-knockout pigs expressing multiple human proteins in cynomolgus macaques.

Applications

Unspecified application

Species

Unspecified reactive species

David Ma,Takayuki Hirose,Grace Lassiter,Hajime Sasaki,Ivy Rosales,Taylor M Coe,Charles G Rickert,Rudy Matheson,Robert B Colvin,Wenning Qin,Yinan Kan,Jacob V Layer,Violette B Paragas,Kathryn Stiede,Katherine C Hall,Michele E Youd,Luis M Queiroz,William F Westlin,Michael Curtis,Luhan Yang,James F Markmann,Tatsuo Kawai

Cellular and molecular life sciences : CMLS 78:3637-3656 PubMed33555391

2021

The Gb3-enriched CD59/flotillin plasma membrane domain regulates host cell invasion by Pseudomonas aeruginosa.

Applications

Unspecified application

Species

Unspecified reactive species

Annette Brandel,Sahaja Aigal,Simon Lagies,Manuel Schlimpert,Ana Valeria Meléndez,Maokai Xu,Anika Lehmann,Daniel Hummel,Daniel Fisch,Josef Madl,Thorsten Eierhoff,Bernd Kammerer,Winfried Römer

BioMed research international 2020:2753414 PubMed32337233

2020

Effects of the Bone/Bone Marrow Microenvironments on Prostate Cancer Cells and CD59 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Yan,Yan Li,Shaoju Min,Peng Zhang,Bin Xu,Zhen Wang,Wei Zhang,Jiasheng Chen,Guangheng Luo,Chunxiao Liu

Journal of Cancer 10:4165-4177 PubMed31413735

2019

iTRAQ-based Comparative Serum Proteomic Analysis of Prostate Cancer Patients with or without Bone Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Yan,Binshen Chen,Shaoju Min,Yubo Gao,Yiming Zhang,Peng Xu,Chaoming Li,Jiasheng Chen,Guangheng Luo,Chunxiao Liu

Journal of cellular and molecular medicine 23:5956-5969 PubMed31293077

2019

Differentiation of human adipose derived stem cells into Leydig-like cells with molecular compounds.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Chen,Chao Li,Weiping Ji,Long Wang,Xianwu Chen,Shenzhi Zhao,Zhangye Xu,Renshan Ge,Xiaoling Guo

Cell 177:1738-1756.e23 PubMed31104842

2019

Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph Mathew Kalappurakkal,Anupama Ambika Anilkumar,Chandrima Patra,Thomas S van Zanten,Michael P Sheetz,Satyajit Mayor

Oncology letters 15:9061-9068 PubMed29805637

2018

Effect of membrane-bound complement regulatory proteins on tumor cell sensitivity to complement-dependent cytolysis triggered by heterologous expression of the α-gal xenoantigen.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Juan Liao,Ya-Jun Yang,Zhu Wang,Feng Qin,Sheng-Ming Zhu,Hong Zheng,Yan-Ping Wang

Oncology letters 14:2961-2969 PubMed28928834

2017

CD55 and CD59 expression protects HER2-overexpressing breast cancer cells from trastuzumab-induced complement-dependent cytotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Ya-Jun Yang,Zhu Wang,Juan Liao,Mei Liu,Xiao-Rong Zhong,Hong Zheng,Yan-Ping Wang
View all publications

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