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AB234013

Anti-CD59抗体[MACIF/629]

Anti-CD59 antibody [MACIF/629]

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(1 Publication)

Mouse Monoclonal CD59 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CD59.

查看别名

CD59, MIC11, MIN1, MIN2, MIN3, MSK21, CD59 glycoprotein, 1F5 antigen, 20 kDa homologous restriction factor, MAC-inhibitory protein, MEM43 antigen, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin, HRF-20, HRF20, MAC-IP, MACIF, MIRL

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MACIF/629] (AB234013)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MACIF/629] (AB234013)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD59 using ab234013 at 2 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MACIF/629] (AB234013)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MACIF/629] (AB234013)

Formalin-fixed, paraffin-embedded human tongue tissue stained for CD59 using ab234013 at 2 μg/ml in immunohistochemical analysis.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

MACIF/629

亚型

IgG1

轻链类型

kappa

不含载体蛋白

No

反应种属

Human

应用

IHC-P

applications

免疫原

Recombinant Full Length Protein corresponding to Human CD59.

P13987

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Primary incubation for 30 minutes at room temperature.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein G
纯化说明
Purified from Bioreactor Concentrate.
存储溶液
Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD59 also known as protectin is a protein with a molecular mass of approximately 18-25 kDa. It is widely expressed on the surface of many human cells including red blood cells and various types of leukocytes. CD59 serves as an inhibitor of the complement membrane attack complex (MAC) preventing cell lysis caused by terminal complement proteins. Through this mechanism it regulates complement activation and maintains cell integrity by halting the formation of MAC.
Biological function summary

One important feature of CD59 is its role as a glycosylphosphatidylinositol (GPI)-anchored protein involved in safeguarding cells from complement-mediated damage. It does not exist within a larger complex but functions at the cell surface to inhibit the assembly of complement components C5b-9 which form the MAC. This ability to inhibit MAC is essential in maintaining self-tissue from unintended damage during immune responses.

Pathways

CD59 participates in the regulation of the complement cascade specifically within the terminal pathway. The complement system serves as a bridge between innate and adaptive immunity contributing to processes like opsonization and cell lysis. CD59's inhibitory action directly impacts pathways that utilize terminal components such as C5b. Effective CD59 function prevents excessive complement activation ensuring that an immune response does not damage host tissues. It links closely with other complement regulatory proteins like CD55 which also mitigate complement cascade activation.

Deficiencies or malfunctions in CD59 have notable implications. Paroxysmal nocturnal hemoglobinuria (PNH) is a disorder where lack of CD59 expression on blood cells leads to their increased destruction due to unregulated complement activity. Furthermore CD59's dysfunction or inadequacy also associates with atypical hemolytic uremic syndrome (aHUS) which involves mutant complement regulatory proteins causing overactivation of the complement system. These associations highlight the importance of CD59 in both maintaining cellular health and preventing pathophysiological conditions related to complement overactivity.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
See full target information CD59
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 50:364-381 PubMed40589547

2025

In situ implantation of type II collagen-based double-layer scaffolds for Articular Osteochondral Regeneration comprising hyaline cartilage and vascularized subchondral bones.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Zhang,Ye Huang,Xu Hu,Yulei Mu,Huiqun Zhou,Liang Ma,Bangheng Liu,Hang Yao,Xieyuan Jiang,Dong-An Wang
View all publications
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搭配使用产品

Assay Kits

AB263893

Human CD59 ELISA Kit

Recombinant|SimpleStep

ELISA - Human CD59 ELISA Kit (AB263893)

  • 0
  • 0
Primary Antibodies

AB133684

Anti-CD55 antibody [EPR6689]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] (AB133684)

  • 5
  • 1
Primary Antibodies

AB108307

Anti-CD46 antibody [EPR4014]

KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (AB108307)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB200999

Anti-C3 antibody [EPR19394]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-C3 antibody [EPR19394] (AB200999)

  • 4
  • 9

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