重组Anti-CD59抗体[EPR6425(2)] (ab133707)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6425(2)] to CD59
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD59抗体[EPR6425(2)]
参阅全部 CD59 一抗 -
描述
兔单克隆抗体[EPR6425(2)] to CD59 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HUVEC and BxPC3 cell lysates; Human placenta and tonsil tissues
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6425(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-CD59 antibody [EPR6425(2)] - BSA and Azide free (ab248625)
- APC Anti-CD59 antibody [EPR6425(2)] (ab319256)
- PE Anti-CD59 antibody [EPR6425(2)] (ab319404)
- Alexa Fluor® 488 Anti-CD59 antibody [EPR6425(2)] (ab319538)
- Alexa Fluor® 647 Anti-CD59 antibody [EPR6425(2)] (ab319651)
- Alexa Fluor® 594 Anti-CD59 antibody [EPR6425(2)] (ab319755)
- Alexa Fluor® 555 Anti-CD59 antibody [EPR6425(2)] (ab319897)
- Alexa Fluor® 750 Anti-CD59 antibody [EPR6425(2)] (ab321064)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133707于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. -
疾病相关
Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300]. -
序列相似性
Contains 1 UPAR/Ly6 domain. -
翻译后修饰
N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. -
细胞定位
Cell membrane. Secreted. Soluble form found in a number of tissues. - Information by UniProt
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数据库链接
- Entrez Gene: 966 Human
- Omim: 107271 Human
- SwissProt: P13987 Human
- Unigene: 278573 Human
- Unigene: 709466 Human
- Unigene: 710641 Human
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别名
- 16.3A5 antibody
- 1F5 antibody
- 1F5 antigen antibody
see all
图片
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Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
Lane 2: CD59 knockout HAP1 whole cell lysate (40 µg)Lanes 1 - 2: Merged signal (red and green). Green - ab133707 observed at 14 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab133707 was shown to specifically react with CD59 in wild-type HAP1 cells as signal was lost in CD59 knockout cells. Wild-type and CD59 knockout samples were subjected to SDS-PAGE. Ab133707 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-CD59 antibody [EPR6425(2)] (ab133707) at 1/1000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : BxPC 3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 14 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] (ab133707)
Immunohistochemical analysis of CD59 in paraffin embedded Human placenta tissue labelled with ab133707 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] (ab133707)
Immunohistochemical analysis of CD59 in paraffin embedded Human tonsil tissue labelled with ab133707 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab133707 被引用在 4 文献中.
- Ge X et al. Complement and complement regulatory proteins are upregulated in lungs of COVID-19 patients. Pathol Res Pract 247:154519 (2023). PubMed: 37244049
- Chen Y et al. Engineered extracellular vesicles for concurrent Anti-PDL1 immunotherapy and chemotherapy. Bioact Mater 9:251-265 (2022). PubMed: 34820569
- Clark AJ & Petty HR Identification of lesion subtypes in biopsies of ductal carcinoma in situ of the breast using biomarker ratio imaging microscopy. Sci Rep 6:27039 (2016). PubMed: 27247112
- Clark AJ & Petty HR Protocol for Biomarker Ratio Imaging Microscopy with Specific Application to Ductal Carcinoma In situ of the Breast. Front Cell Dev Biol 4:120 (2016). PubMed: 27857940