重组Anti-CD59抗体[EPR28954-50] - BSA and Azide free

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD59 antibody [EPR28954-50] - BSA and Azide free (AB318141)

This data was developed using ab318140, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD59 with ab318140 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti-CD4 conjugated to Alexa Fluor®647.
Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD59 antibody [EPR28954-50] - BSA and Azide free (AB318141)

This data was developed using ab318140, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Daudi (human Burkitt's lymphoma lymphoblast, Left) / BxPC-3 (human pancreas adenocarcinoma epithelial cell, Right) cells labelling CD59 with ab318140 at 1/500 dilution (0.1 ug)/Magenta compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Low expression : Daudi(PMID : 2475570)
Gated on viable cell.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD59 antibody [EPR28954-50] - BSA and Azide free (AB318141)

This data was developed using ab318140, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized CD59 KO HAP1 (CD59 knockout human chronic myelogenous leukemia near-haploid cell) cells labelling CD59 with ab318140 at 1/100 (4.83 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing mainly membranous staining in parental HAP1 cell line and negative staining in CD59 KO HAP1 (shown in green).
The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD59 antibody [EPR28954-50] - BSA and Azide free (AB318141)

This data was developed using ab318140, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Wild-type HAP1(human chronic myelogenous leukemia near-haploid cell, Left) / CD59 knockout HAP1(Right) cells labelling CD59 with ab318140 at 1/500 dilution (0.1 ug)/Magenta compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cell.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD59 antibody [EPR28954-50] - BSA and Azide free (AB318141)

This data was developed using ab318140, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized BxPC-3 (human pancreas adenocarcinoma epithelial cell) cells labelling CD59 with ab318140 at 1/100 (4.83 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing membranous and weak cytoplasmic staining in BxPC-3 cell line (shown in green).
Low expression : Daudi (PMID : 2475570)
The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.