重组Anti-CD59抗体[EPR28954-50] (ab318140)
![Immunocytochemistry/ Immunofluorescence - Anti-CD59 antibody [EPR28954-50] (ab318140)](https://www.abcam.cn/ps/products/318/ab318140/Images/ab318140-1-anti-cd59-antibody-epr28954-50-immunocytochemistry-cd59-ko-hap1.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD59 antibody [EPR28954-50] (ab318140)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR28954-50] to CD59
- Suitable for: ICC/IF, Flow Cyt
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD59抗体[EPR28954-50]
参阅全部 CD59 一抗 -
描述
兔单克隆抗体[EPR28954-50] to CD59 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, Flow Cytmore details
不适用于: IHC-P,IP or WB -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: Parental HAP1 cell line and BxPC-3 cells. Flow Cyt: Wild-type HAP1, BxPC-3 and Human PBMC cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR28954-50 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab318140于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100.
|
|
| Flow Cyt |
1/500.
|
| 说明 |
|---|
|
ICC/IF
1/100. |
|
Flow Cyt
1/500. |
靶标
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功能
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. -
疾病相关
Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300]. -
序列相似性
Contains 1 UPAR/Ly6 domain. -
翻译后修饰
N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. -
细胞定位
Cell membrane. Secreted. Soluble form found in a number of tissues. - Information by UniProt
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数据库链接
- Entrez Gene: 966 Human
- Omim: 107271 Human
- SwissProt: P13987 Human
- Unigene: 278573 Human
- Unigene: 709466 Human
- Unigene: 710641 Human
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别名
- 16.3A5 antibody
- 1F5 antibody
- 1F5 antigen antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-CD59 antibody [EPR28954-50] (ab318140)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized CD59 KO HAP1 (CD59 knockout human chronic myelogenous leukemia near-haploid cell) cells labelling CD59 with ab318140 at 1/100 (4.83 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing mainly membranous staining in parental HAP1 cell line and negative staining in CD59 KO HAP1 (shown in green).
The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD59 antibody [EPR28954-50] (ab318140)](https://www.abcam.cn/ps/products/318/ab318140/Images/ab318140-2-anti-cd59-antibody-epr28954-50-immunocytochemistry-bxpc-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD59 antibody [EPR28954-50] (ab318140)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized BxPC-3 (human pancreas adenocarcinoma epithelial cell) cells labelling CD59 with ab318140 at 1/100 (4.83 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing membranous and weak cytoplasmic staining in BxPC-3 cell line (shown in green).
Low expression: Daudi (PMID:2475570)
The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
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![Flow Cytometry - Anti-CD59 antibody [EPR28954-50] (ab318140)](https://www.abcam.cn/ps/products/318/ab318140/Images/ab318140-4-anti-cd59-antibody-epr28954-50-flow-cytometry-daudi.jpg)
Flow Cytometry - Anti-CD59 antibody [EPR28954-50] (ab318140)Flow cytometric analysis of Daudi (human Burkitt's lymphoma lymphoblast, Left) / BxPC-3 (human pancreas adenocarcinoma epithelial cell, Right) cells labelling CD59 with ab318140 at 1/500 dilution (0.1 ug)/Magenta compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Low expression: Daudi(PMID: 2475570)
Gated on viable cell. -
![Flow Cytometry - Anti-CD59 antibody [EPR28954-50] (ab318140)](https://www.abcam.cn/ps/products/318/ab318140/Images/ab318140-543928-anti-cd59-antibody-epr28954-50-flow-cytometry-wild-type-hap1.jpg)
Flow Cytometry - Anti-CD59 antibody [EPR28954-50] (ab318140)Flow cytometric analysis of Wild-type HAP1(human chronic myelogenous leukemia near-haploid cell, Left) / CD59 knockout HAP1(Right) cells labelling CD59 with ab318140 at 1/500 dilution (0.1 ug)/Magenta compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cell.
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![Flow Cytometry - Anti-CD59 antibody [EPR28954-50] (ab318140)](https://www.abcam.cn/ps/products/318/ab318140/Images/ab318140-5-anti-cd59-antibody-epr28954-50-flow-cytometry-human-pbmc.jpg)
Flow Cytometry - Anti-CD59 antibody [EPR28954-50] (ab318140)Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD59 with ab318140 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Cells were co-stained with anti-CD4 conjugated to Alexa Fluor®647.
Gated on viable cells. -
![Anti-CD59 antibody [EPR28954-50] (ab318140)](https://www.abcam.cn/ps/products/318/ab318140/Images/ab318140-6-benefitsofrecombinantantibodies.jpg)
Anti-CD59 antibody [EPR28954-50] (ab318140)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab318140 尚未被引用在任何文献中。