Anti-CD55抗体[MEM-118] (ab1422)
![Flow Cytometry - Anti-CD55 antibody [MEM-118] (ab1422)](https://www.abcam.cn/ps/products/1/ab1422/Images/ab1422-1-ab1422FC.jpg "Flow Cytometry - Anti-CD55 antibody [MEM-118] (ab1422)")
Key features and details
- Mouse monoclonal [MEM-118] to CD55
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgM
Related conjugates and formulations
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CD55抗体[MEM-118]
参阅全部 CD55 一抗 -
描述
小鼠单克隆抗体[MEM-118] to CD55 -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human
预测可用于: Non human primates
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免疫原
Tissue, cells or virus corresponding to Human CD55. T cell line HPB-ALL
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常规说明
This product was changed from ascites to tissue culture supernatant on 24th Januray 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 8.0
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Proprietary Purification -
纯化说明
Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
MEM-118 -
同种型
IgM -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use a concentration of 10 µg/ml.
|
| 说明 |
|---|
|
Flow Cyt
Use a concentration of 10 µg/ml. |
靶标
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功能
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. -
组织特异性
Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix. -
序列相似性
Belongs to the receptors of complement activation (RCA) family.
Contains 4 Sushi (CCP/SCR) domains. -
结构域
The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3. -
翻译后修饰
The Ser/Thr-rich domain is heavily O-glycosylated. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1604 Human
- Omim: 125240 Human
- SwissProt: P08174 Human
- Unigene: 126517 Human
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别名
- CD 55 antibody
- CD55 antibody
- CD55 antigen antibody
see all
图片
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Flow Cytometry - Anti-CD55 antibody [MEM-118] (ab1422)
Human peripheral blood lymphocytes stained with ab1422 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab1422, 1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab1422 被引用在 6 文献中.
- Gaykema LH et al. Inhibition of complement activation by CD55 overexpression in human induced pluripotent stem cell derived kidney organoids. Front Immunol 13:1058763 (2022). PubMed: 36713440
- Trimova G et al. Tumour necrosis factor alpha promotes secretion of 14-3-3? by inducing necroptosis in macrophages. Arthritis Res Ther 22:24 (2020). PubMed: 32051018
- Cheng L et al. Complement regulatory proteins in kidneys of patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis. Clin Exp Immunol 191:116-124 (2018). PubMed: 28940198
- Kapka-Skrzypczak L et al. Effect of IL-6 and IL-8 on the expression of the complement activation inhibitors MAC-inhibitory protein and decay-accelerating factor in ovarian cancer A2780 cells. Oncol Lett 12:1507-1512 (2016). ICC/IF ; Human . PubMed: 27446461
- Lee MS et al. Exploitation of the complement system by oncogenic Kaposi's sarcoma-associated herpesvirus for cell survival and persistent infection. PLoS Pathog 10:e1004412 (2014). ICC/IF ; Human . PubMed: 25254972
- Cinek T & Horejsi V The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases. J Immunol 149:2262-70 (1992). PubMed: 1382093