Anti-CD53抗体[MRC OX-44] (ab22383)
![Flow Cytometry - Anti-CD53 antibody [MRC OX-44] (ab22383)](https://www.abcam.cn/ps/products/22/ab22383/Images/ab22383-1-ab22383Flow1.jpg "Flow Cytometry - Anti-CD53 antibody [MRC OX-44] (ab22383)")
Key features and details
- Mouse monoclonal [MRC OX-44] to CD53
- Suitable for: Flow Cyt
- Reacts with: Rat
- Isotype: IgG1
Related conjugates and formulations
概述
-
产品名称
Anti-CD53抗体[MRC OX-44]
参阅全部 CD53 一抗 -
描述
小鼠单克隆抗体[MRC OX-44] to CD53 -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Rat -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Flow Cyt: Lewis rat splenocytes.
-
常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified from TCS. -
Primary antibody说明
The antibody recognises the rat equivalent of human CD11b, the receptor for the iC3b component of complement. The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labelled dendritic cells, granulocytes and microglial cells in the brain. -
克隆
单克隆 -
克隆编号
MRC OX-44 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab22383于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use a concentration of 0.2 µg/ml.
|
| 说明 |
|---|
|
Flow Cyt
Use a concentration of 0.2 µg/ml. |
靶标
-
功能
May be involved in growth regulation in hematopoietic cells. -
组织特异性
B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells. -
序列相似性
Belongs to the tetraspanin (TM4SF) family. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 24251 Rat
- SwissProt: P24485 Rat
- Unigene: 31988 Rat
-
别名
- AI323659 antibody
- Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody
- CD 53 antibody
see all
图片
-
Flow Cytometry - Anti-CD53 antibody [MRC OX-44] (ab22383)
Lewis rat splenocytes stained with ab22383 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab22383) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab22383 被引用在 2 文献中.
- Tomlinson MG et al. Epitope mapping of anti-rat CD53 monoclonal antibodies. Implications for the membrane orientation of the Transmembrane 4 Superfamily. Eur J Immunol 23:136-40 (1993). PubMed: 7678222
- Paterson DJ et al. The MRC OX-44 antigen marks a functionally relevant subset among rat thymocytes. J Exp Med 165:1-13 (1987). PubMed: 3098892