重组Anti-CD53抗体[EPR4342(2)] (ab134094)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4342(2)] to CD53
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD53抗体[EPR4342(2)]
参阅全部 CD53 一抗 -
描述
兔单克隆抗体[EPR4342(2)] to CD53 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, Flow Cyt (Intra)more details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Raji, PBMC, and Jurkat whole cell lysate (ab7899); Human tonsil tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4342(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134094于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 24 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
靶标
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功能
May be involved in growth regulation in hematopoietic cells. -
组织特异性
B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells. -
序列相似性
Belongs to the tetraspanin (TM4SF) family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 963 Human
- Omim: 151525 Human
- SwissProt: P19397 Human
- Unigene: 443057 Human
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别名
- AI323659 antibody
- Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody
- CD 53 antibody
see all
图片
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All lanes : Anti-CD53 antibody [EPR4342(2)] (ab134094) at 1/5000 dilution
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate. lysate was treated with Deglycosylase
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and dilution buffer: 5% NFDM/TBST.
We are not sure how to define the extra band.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD53 antibody [EPR4342(2)] (ab134094)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD53 with ab134094 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-CD53 antibody [EPR4342(2)] (ab134094) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : PBMC cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 24 kDa -
Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling CD53 with unpurified ab134094 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab134094 被引用在 2 文献中.
- Ferguson ID et al. The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance. Nat Commun 13:4121 (2022). PubMed: 35840578
- Montanaro M et al. The Paradox Effect of Calcification in Carotid Atherosclerosis: Microcalcification is Correlated with Plaque Instability. Int J Mol Sci 22:N/A (2021). PubMed: 33401449