AB277141

重组Anti-CD48抗体[341] - BSA and Azide free

Anti-CD48 antibody [341] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal CD48 antibody. Carrier free. Suitable for ICC, Flow Cyt and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Cd48 aa 1 to C-terminus.

查看别名

CD48, Bcm-1, Cd48, CD48 antigen, BCM1 surface antigen, BLAST-1, HM48-1, MRC OX-45 surface antigen, SLAM family member 2, Signaling lymphocytic activation molecule 2, sgp-60, SLAMF2

2 Images

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Immunocytochemistry - Anti-CD48 antibody [341] - BSA and Azide free (AB277141)

Immunofluorescence staining of CD48 in mouse spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with ab277141 at 1/60 dilution at 37°C for 1 hour. Then cells were stained with the Alexa Fluor^® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).

Flow Cytometry - Anti-CD48 antibody [341] - BSA and Azide free (AB277141)

Flow Cyt

Unknown

Flow Cytometry - Anti-CD48 antibody [341] - BSA and Azide free (AB277141)

Flow cytometric analysis of CD48 expression on mouse BABL/c splenocytes. Cells were stained with ab277141 at 1/25 dilution, then a FITC-conjugated second step antibody (Black). Cells were also staibed with an isotype control (Grey). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

341

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse

应用

ICC, Flow Cyt

applications

免疫原

Recombinant Fragment Protein within Mouse Cd48 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P18181

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/20 - 1/100", "ICC-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/25 - 1/100", "FlowCyt-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD48 also known as SLAMF2 or B-lymphocyte activation marker BLAST-1 is a glycosylphosphatidylinositol (GPI)-anchored protein with a molecular mass approximately 45-70 kDa depending on glycosylation. It is expressed on the surface of various immune cells like T cells B cells and natural killer (NK) cells. CD48 does not possess an intracellular signaling domain but engages in extracellular interactions with other proteins affecting immune cell activities.

Biological function summary

CD48 serves as a co-stimulatory molecule involved in immune response modulation. It interacts with 2B4 (CD244) a member of the signaling lymphocyte activation molecule (SLAM) family to form a receptor-ligand complex. This interaction can result in enhanced T cell and NK cell responses. CD48's presence on activated hematopoietic and non-hematopoietic cells highlights its role in cell-to-cell communication.

Pathways

CD48 participates in SLAM family receptor signaling and natural killer cell-mediated cytotoxicity. In the SLAM pathway CD48 enhances communication and regulation between T cells and NK cells. This communication also relates to the roles of CD244 linking it to downstream signaling pathways that influence immune cell function. These pathways illustrate the critical network CD48 is part of which impacts various immune responses.

CD48 is involved in autoimmune conditions like rheumatoid arthritis and immunological cancers such as lymphoma. In rheumatoid arthritis CD48 along with CD244 modulates immune responses which can exacerbate inflammation. In lymphoma abnormal CD48 expression can contribute to tumor progression and immune evasion indicating its potential as a therapeutic target.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that interacts via its N-terminal immunoglobulin domain with cell surface receptors including 2B4/CD244 or CD2 to regulate immune cell function and activation (PubMed : 21278219, PubMed : 9881969). Participates in T-cell signaling transduction by associating with CD2 and efficiently bringing the Src family protein kinase LCK and LAT to the TCR/CD3 complex. In turn, promotes LCK phosphorylation and subsequent activation (PubMed : 9881969). Induces the phosphorylation of the cytoplasmic immunoreceptortyrosine switch motifs (ITSMs) of CD244 initiating a series of signaling events that leads to the generation of the immunological synapse and the directed release of cytolytic granules containing perforin and granzymes by T-lymphocytes and NK-cells (By similarity) (PubMed : 21278219, PubMed : 9881969).

See full target information Cd48

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重组Anti-CD48抗体[341] - BSA and Azide free

Anti-CD48 antibody [341] - BSA and Azide free

Immunocytochemistry - Anti-CD48 antibody [341] - BSA and Azide free (AB277141)

Flow Cytometry - Anti-CD48 antibody [341] - BSA and Azide free (AB277141)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原