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使用敲除细胞株进行验证重组RabMAb

重组Anti-CD46抗体[EPR4014] (ab108307)

Reviews (1)Q&A (2)References (16)
Western blot - Anti-CD46 antibody [EPR4014] (ab108307)
  • Western blot - Anti-CD46 antibody [EPR4014] (ab108307)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)
  • Western blot - Anti-CD46 antibody [EPR4014] (ab108307)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)
  • Anti-CD46 antibody [EPR4014] (ab108307)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4014] to CD46
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-CD46抗体[EPR4014]
    参阅全部 CD46 一抗

  • 描述

    兔单克隆抗体[EPR4014] to CD46

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt or IP

  • 种属反应性

    与反应: Human
    预测可用于: Rabbit

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Wild-type HAP1 cell lysate. MOLT-4, Jurkat, HeLa and K562 cell lysates. IHC-P: Human thyroid gland carcinoma, colonic adenocarcinoma, breast carcinoma, kidney, breast, uterus, placenta and tonsil tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR4014

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab108307于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
IHC-P (1)
1/500 - 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
应用说明
Is unsuitable for Flow Cyt or IP.

靶标

  • 功能

    Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.

  • 组织特异性

    Expressed by all cells except erythrocytes.

  • 疾病相关

    Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2) [MIM:612922]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations.

  • 序列相似性

    Contains 4 Sushi (CCP/SCR) domains.

  • 结构域

    Sushi domains 1 and 2 are required for interaction with human adenovirus B PIV/FIBER protein and with Measles virus H protein. Sushi domains 2 and 3 are required for Herpesvirus 6 binding. Sushi domain 3 is required for Neisseria binding. Sushi domains 3 and 4 are required for interaction with Streptococcus pyogenes M protein and are the most important for interaction with C3b and C4b.

  • 翻译后修饰

    N-glycosylated on Asn-83; Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding.
    Extensively O-glycosylated in the Ser/Thr-rich domain. O-glycosylation is required for Neisseria binding but not for Measles virus or human adenovirus binding.
    In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae; which promotes infectivity. In T-cells, these isoforms may be phosphorylated by Lck.

  • 细胞定位

    Cytoplasmic vesicle > secretory vesicle > acrosome inner membrane. Inner acrosomal membrane of spermatozoa. Internalized upon binding of Measles virus, Herpesvirus 6 or Neisseria gonorrhoeae, which results in an increased susceptibility of infected cells to complement-mediated injury. In cancer cells or cells infected by Neisseria, shedding leads to a soluble peptide.
  • Information by UniProt

  • 数据库链接

    • 别名

      • AHUS2 antibody
      • Antigen defined by monoclonal antibody TRA 2 10 antibody
      • Antigen identified by monoclonal antibody TRA 2 10 antibody
      • CD46 antibody
      • CD46 antigen antibody
      • CD46 antigen complement regulatory protein antibody
      • CD46 molecule antibody
      • CD46 molecule complement regulatory protein antibody
      • Complement membrane cofactor protein antibody
      • MCP antibody
      • MCP_HUMAN antibody
      • Measles virus receptor antibody
      • membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) antibody
      • Membrane cofactor protein antibody
      • MGC26544 antibody
      • MIC10 antibody
      • TLX antibody
      • TRA2.10 antibody
      • Trophoblast leucocyte common antigen antibody
      • Trophoblast leukocyte common antigen antibody
      • Trophoblast lymphocyte cross reactive antigen antibody
      see all

    图片

    • Western blot - Anti-CD46 antibody [EPR4014] (ab108307)
      Western blot - Anti-CD46 antibody [EPR4014] (ab108307)
      All lanes : Anti-CD46 antibody [EPR4014] (ab108307) at 1/1000 dilution

      Lane 1 : Wild-type A549 cell lysate
      Lane 2 : CD46 knockout A549 cell lysate
      Lane 3 : HAP1 cell lysate
      Lane 4 : THP-1 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 44 kDa
      Observed band size: 50-75 kDa why is the actual band size different from the predicted?



      Western blot: Anti-CD46 antibody [EPR4014] (ab108307) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab108307 was shown to bind specifically to CD46. A band was observed at 50-75 kDa in wild-type A549 cell lysates with no signal observed at this size in CD46 knockout cell line. To generate this image, wild-type and CD46 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    • Western blot - Anti-CD46 antibody [EPR4014] (ab108307)
      Western blot - Anti-CD46 antibody [EPR4014] (ab108307)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: CD46 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: K562 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab108307 observed at 50-70 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab108307 was shown to specifically react with  when CD46 knockout samples were used. Wild-type and CD46 knockout samples were subjected to SDS-PAGE. ab108307 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)

      ab108307, at 1/500 dilution, staining CD46 in paraffin-embedded human tonsil tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)

      ab108307, at 1/500 dilution, staining CD46 in paraffin-embedded human kidney tissue

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Western blot - Anti-CD46 antibody [EPR4014] (ab108307)
      Western blot - Anti-CD46 antibody [EPR4014] (ab108307)
      All lanes : Anti-CD46 antibody [EPR4014] (ab108307) at 1/1000 dilution

      Lane 1 : MOLT-4 (Human lymphoblastic leukemia cell line) cell lysate
      Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
      Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
      Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution

      Predicted band size: 44 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)

      ab108307 showing positive staining in human normal placenta tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)

      ab108307 showing positive staining in human normal uterus tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)

      ab108307 showing positive staining in human thyroid gland carcinoma tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)

      ab108307 showing positive staining in human colonic adenocarcinoma tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)

      ab108307 showing positive staining in human breast carcinoma tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)

      ab108307 showing positive staining in human normal breast tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Anti-CD46 antibody [EPR4014] (ab108307)
      Anti-CD46 antibody [EPR4014] (ab108307)

    文献 (16)

    发表研究结果有使用 ab108307？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab108307 被引用在 16 文献中.

    • Jiang Q  et al. Effects of Plasma-Derived Exosomal miRNA-19b-3p on Treg/T Helper 17 Cell Imbalance in Behçet's Uveitis. Invest Ophthalmol Vis Sci 64:28 (2023). PubMed: 37093132
    • Ge X  et al. Complement and complement regulatory proteins are upregulated in lungs of COVID-19 patients. Pathol Res Pract 247:154519 (2023). PubMed: 37244049
    • Ma D  et al. Kidney transplantation from triple-knockout pigs expressing multiple human proteins in cynomolgus macaques. Am J Transplant 22:46-57 (2022). PubMed: 34331749
    • Wang J  et al. Patient with CATSPER3 mutations-related failure of sperm acrosome reaction with successful pregnancy outcome from intracytoplasmic sperm injection (ICSI). Mol Genet Genomic Med 9:e1579 (2021). PubMed: 33350607
    • Huang Y  et al. A SIRPa-Fc fusion protein enhances the antitumor effect of oncolytic adenovirus against ovarian cancer. Mol Oncol 14:657-668 (2020). PubMed: 31899582
    View all Publications for this product

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD46 antibody [EPR4014]

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (tonsil, intestine)
    Specification
    tonsil, intestine
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris buffer (Ventana)
    Permeabilization
    No
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Mar 06 2012

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