为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Immunology Adaptive Immunity B Cells CD

Anti-CD45RO抗体[UCH-L1] (ab23)

  • Datasheet
  • SDS
Reviews (3)Q&A (3)References (15)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RO antibody [UCH-L1] (ab23)
  • Western blot - Anti-CD45RO antibody [UCH-L1] (ab23)

Key features and details

  • Mouse monoclonal [UCH-L1] to CD45RO
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

HRP

你可能会感兴趣

一抗
Product image
Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)
一抗
Product image
Anti-Liver Arginase antibody [EPR6672(B)] - BSA and Azide free (ab211961)
偶联
Product image
Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

查看更多相关产品

概述

  • 产品名称

    Anti-CD45RO抗体[UCH-L1]
    参阅全部 CD45RO 一抗
  • 描述

    小鼠单克隆抗体[UCH-L1] to CD45RO
  • 宿主

    Mouse
  • 经测试应用

    适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Tissue, cells or virus corresponding to CD45RO. Cultured T cells from an IL-2-dependent T-cell line (CA1) prepared from human peripheral blood activated with influenza virus.

  • 阳性对照

    • IHC-P: Human tonsil tissue. WB: Human tonsil and thymus tissue lysate.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.2% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    UCH-L1
  • 骨髓瘤

    P3-NS1/1-Ag4-1
  • 同种型

    IgG2a
  • 研究领域

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Neuroscience
    • Processes

相关产品

  • Alternative Versions

    • HRP Anti-CD45RO antibody [UCH-L1] (ab187836)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab23于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa).
IHC-P (3)
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
说明
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa).
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

靶标

  • 功能

    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
  • 疾病相关

    Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • 序列相似性

    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • 结构域

    The first PTPase domain interacts with SKAP1.
  • 翻译后修饰

    Heavily N- and O-glycosylated.
  • 细胞定位

    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Target information above from: UniProt accession P08575 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 5788 Human
    • Omim: 151460 Human
    • SwissProt: P08575 Human
    • Unigene: 654514 Human
    • 别名

      • B220 antibody
      • CD45 antibody
      • cd45 antigen antibody
      • CD45R antibody
      • GP180 antibody
      • L-CA antibody
      • lca antibody
      • Leukocyte common antigen antibody
      • LY 5 antibody
      • LY5 antibody
      • Protein tyrosine phosphatase, receptor type, C antibody
      • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
      • Ptprc antibody
      • PTPRC_HUMAN antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • T200 antibody
      • T200 glycoprotein antibody
      • T200 leukocyte common antigen antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RO antibody [UCH-L1] (ab23)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RO antibody [UCH-L1] (ab23)

      ab23 (1 µg/ml) staining CD45RO in human tonsil.
      Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH 6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with hematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    • Western blot - Anti-CD45RO antibody [UCH-L1] (ab23)
      Western blot - Anti-CD45RO antibody [UCH-L1] (ab23)
      All lanes : Anti-CD45RO antibody [UCH-L1] (ab23) at 5 µg/ml

      Lane 1 : Human tonsil normal tissue lysate - total protein (ab29615)
      Lane 2 : Human thymus tissue lysate - total protein (ab30146)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 131 kDa
      Observed band size: 180 kDa why is the actual band size different from the predicted?
      Additional bands at: 120 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 8 minutes


      CD45RO contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    实验方案

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (15)

    发表研究结果有使用 ab23?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab23 被引用在 15 文献中.

    • Yang W  et al. ACE2 Correlated With Immune Infiltration Serves As A Novel Prognostic Biomarker In Clear Cell Renal Cell Carcinoma: Implication For COVID-19. Int J Biol Sci 17:20-31 (2021). PubMed: 33390830
    • Kim HD  et al. Spatial Distribution and Prognostic Implications of Tumor-Infiltrating FoxP3- CD4+ T Cells in Biliary Tract Cancer. Cancer Res Treat 53:162-171 (2021). PubMed: 32878426
    • Huang KC  et al. Prognostic relevance of programmed cell death 1 ligand 2 (PDCD1LG2/PD-L2) in patients with advanced stage colon carcinoma treated with chemotherapy. Sci Rep 10:22330 (2020). PubMed: 33339860
    • Patibandla C  et al. Costus pictus D. Don leaf extract stimulates GLP-1 secretion from GLUTag L-cells and has cytoprotective effects in BRIN-BD11 ß-cells. J Ethnopharmacol 260:112970 (2020). PubMed: 32422353
    • Zhang WH  et al. Infiltrating pattern and prognostic value of tertiary lymphoid structures in resected non-functional pancreatic neuroendocrine tumors. J Immunother Cancer 8:N/A (2020). PubMed: 33055204
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD45RO antibody [UCH-L1]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    tonsil
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
    Fixative
    10% NBF
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 12 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD45RO antibody [UCH-L1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    No
    Specification
    Tonsil
    Blocking step
    Bloxall Endogenous Peroxidase and Alkaline Phosphatase Blocking as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Joshua Dodge

    Verified customer

    提交于 Jan 22 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD45RO antibody [UCH-L1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lymph node)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA
    Permeabilization
    No
    Specification
    Lymph node
    Blocking step
    Casein as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 30 2015

    Question

    Please tell us the necessary dilution for this product ab23 Mouse monoclonal [UCH-L1] to CD45RO lot no GR114857, for IHC-P use.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 20 2013

    Answer


    The concentration for this lot is 1 mg/ml. We haven't specified a dilution range for this product in IHC-P however on the datasheet, IHC-P was successfully performed using 1 ug/ml therefore a 1/1000 dilution. I would suggest starting with this dilution and then optimising it for your particular experiment.

    Read More

    Abcam Scientific Support

    回复于 Mar 20 2013

    Question

    Dear Sir/Madam˜

    I hope you have a great day.


    Does ab23 contain any BSA?

    Our customers do not need BSA containg on these products.


    .

    Read More

    Abcam community

    Verified customer

    Asked on Jul 03 2012

    Answer

    Thank you for contacting us. No, ab23 does not contain any BSA, the antibody is in PBS only.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    回复于 Jul 03 2012

    Question

    Does the CD45RO (clone UCH-L1) cross-react with cynomolgus?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 21 2001

    Answer

    We have not had any reports of this antibody being used with cynamologous and the nature of the epitope is unknown. A reference to the immunodetection of CD45RO in cynaomolgous as shown below: Li SL et al, Res Virol 1991 Mar-Jun;142(2-3):173-82

    Read More

    Abcam Scientific Support

    回复于 Feb 21 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2023 艾博抗(上海)贸易有限公司版权所有