Anti-CD45RA抗体[MEM-56] (ab8217)
![Flow Cytometry - Anti-CD45RA antibody [MEM-56] (ab8217)](https://www.abcam.cn/ps/products/8/ab8217/Images/ab8217-521306-anti-cd45ra-antibody-mem-56-flow-cytometry-lymphocytes-human.jpg "Flow Cytometry - Anti-CD45RA antibody [MEM-56] (ab8217)")
Key features and details
- Mouse monoclonal [MEM-56] to CD45RA
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
Anti-CD45RA抗体[MEM-56]
参阅全部 CD45RA 一抗 -
描述
小鼠单克隆抗体[MEM-56] to CD45RA -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD45RA. Thymocytes and T lymphocytes
Database link: P08575-8 -
阳性对照
- WB: HUT-78 human cutaneous T cell lymphoma cell lysate.
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常规说明
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from TCS -
克隆
单克隆 -
克隆编号
MEM-56 -
同种型
IgG2b -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Cross reacts with an intracellular 60 kDa protein of unknown identity like other anti-CD45RA MAbs.
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| IHC-P |
Use at an assay dependent concentration.
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| Flow Cyt |
Use a concentration of 0.3 - 4 µg/ml.
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Cross reacts with an intracellular 60 kDa protein of unknown identity like other anti-CD45RA MAbs. |
|
IHC-P
Use at an assay dependent concentration. |
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Flow Cyt
Use a concentration of 0.3 - 4 µg/ml. |
靶标
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功能
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. -
疾病相关
Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
Multiple sclerosis -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
结构域
The first PTPase domain interacts with SKAP1. -
翻译后修饰
Heavily N- and O-glycosylated. -
细胞定位
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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数据库链接
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
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别名
- B220 antibody
- CD45 antibody
- CD45 antigen antibody
see all
图片
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Flow Cytometry - Anti-CD45RA antibody [MEM-56] (ab8217)
Flow cytometric analysis of Human Peripheral Blood cells labelling CD45RA with ab8217 at 0.3 ug/ml showing separation of human CD45RA positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [MEM-56] (ab8217)](https://www.abcam.cn/ps/products/8/ab8217/Images/ab8217-521307-anti-cd45ra-antibody-mem-56-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [MEM-56] (ab8217)Immunohistochemical analysis of Paraffin-embedded sections Human Tonsil tissue labelling CD45RA with ab8217 at 5µg/ml.
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![Western blot - Anti-CD45RA antibody [MEM-56] (ab8217)](https://www.abcam.cn/ps/products/8/ab8217/Images/ab8217-13659-anti-cd45ra-antibody-mem-56-western-blot.jpg)
Western blot - Anti-CD45RA antibody [MEM-56] (ab8217)Lane 1 : CD45 control antibody
Lanes 2-3 : Anti-CD45RA antibody [MEM-56] (ab8217)
Lane 1 : HUT-78 human cutaneous T cell lymphoma cell line.
Lane 2 : sample of cell lysate prepared under non-reducing conditions
Lane 3 : sample of cell lysate prepared under reducing conditions
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab8217 被引用在 4 文献中.
- Zizzari IG et al. The Macrophage Galactose-Type C-Type Lectin (MGL) Modulates Regulatory T Cell Functions. PLoS One 10:e0132617 (2015). PubMed: 26147970
- Abdulamir AS et al. Changing survival, memory cell compartment, and T-helper balance of lymphocytes between severe and mild asthma. BMC Immunol 9:73 (2008). ICC ; Human . PubMed: 19087256
- Bazil V et al. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha) 35:289-97 (1989). PubMed: 2534480
- Bazil V et al. Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression. Immunogenetics 29:202-5 (1989). PubMed: 2466778