AB8217

Anti-CD45RA 抗体 [MEM-56]

Name: CD45RA抗体[MEM-56] (ab8217)| Abcam中文官网
Brand: Abcam Limited
SKU: AB8217
Price: 4606 CNY
Availability: InStock

Anti-CD45RA antibody [MEM-56]

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(4 Publications)

Mouse Monoclonal CD45 antibody. Suitable for WB, IHC-P, Flow Cyt and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing PTPRC protein.

查看别名

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [MEM-56] (AB8217)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [MEM-56] (AB8217)

Immunohistochemical analysis of Paraffin-embedded sections Human Tonsil tissue labelling CD45RA with ab8217 at 5µg/ml.

Flow Cytometry - Anti-CD45RA antibody [MEM-56] (AB8217)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD45RA antibody [MEM-56] (AB8217)

Flow cytometric analysis of Human Peripheral Blood cells labelling CD45RA with ab8217 at 0.3 ug/ml showing separation of human CD45RA positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed).

Unknown

Western blot - Anti-CD45RA antibody [MEM-56] (AB8217)

Lane 1:

CD45 control antibody

Lanes 2 - 3:

Western blot - Anti-CD45RA antibody [MEM-56] (ab8217)

Lane 1:

HUT-78 human cutaneous T cell lymphoma cell line.

Lane 2:

sample of cell lysate prepared under non-reducing conditions

Lane 3:

sample of cell lysate prepared under reducing conditions

Predicted band size: 147 kDa

false

不同偶联物与剂型 (3)

519 FITC

FITC Anti-CD45RA antibody [MEM-56]
778 PE/Cy7®

PE/Cy7® Anti-CD45RA antibody [MEM-56]
618 PE/DyLight™ 594

PE/DyLight™ 594 Anti-CD45RA antibody [MEM-56]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

MEM-56

亚型

IgG2b

不含载体蛋白

反应种属

Human

应用

WB, Flow Cyt, IHC-P

applications

免疫原

Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information.

P08575

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Cross reacts with an intracellular 60 kDa protein of unknown identity like other anti-CD45RA MAbs.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "No pre-treatment of tissue sections is needed.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.3-4 µg/mL", "FlowCyt-species-notes": "" } } }

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产品详情

This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

纯化说明

Purified from TCS

存储溶液

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD45RA also known as CD45RA antigen and T200 is an isoform of the CD45 protein family. It is a transmembrane protein tyrosine phosphatase with a molecular mass of approximately 220 kDa. CD45RA expression is abundant on naive T-cells and subsets of other immune cells like B cells. Researchers frequently use CD45RA antibodies including CD45RA-PE and CD45RA-FITC for flow cytometry to identify and study immune cell subsets.

Biological function summary

CD45RA plays a significant role in regulating T-cell receptor (TCR) signaling. By dephosphorylating specific phosphotyrosine residues on substrates CD45RA modulates signal transduction pathways important for T-cell activation and differentiation. This protein does not act alone but is part of a regulatory network that influences immune responses and cellular development through its interaction with other cell surface receptors and intracellular kinases.

Pathways

CD45RA modulation is integral to T-cell development and activation processes. It is an important participant in the T-cell receptor signaling pathway and the Jak-STAT signaling pathway. In these pathways CD45 interacts with proteins such as Lck and Fyn which are essential for propagating receptor-mediated signaling events in T-cells. Effective signal regulation through CD45RA is necessary to ensure appropriate immune responses.

CD45RA has links to autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. The aberrant expression or function of CD45RA on immune cells can lead to improper immune activation or suppression contributing to the pathology of these diseases. Crossover with other proteins such as CD45RO which is an isoform involved in memory T-cell function indicates a broader network of immune regulation where CD45RA maintains the balance between immune activation and tolerance.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

See full target information PTPRC

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

PloS one 10:e0132617 PubMed26147970

2015

The Macrophage Galactose-Type C-Type Lectin (MGL) Modulates Regulatory T Cell Functions.

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Grazia Zizzari,Paola Martufi,Federico Battisti,Hassan Rahimi,Salvatore Caponnetto,Filippo Bellati,Marianna Nuti,Aurelia Rughetti,Chiara Napoletano

BMC immunology 9:73 PubMed19087256

2008

Changing survival, memory cell compartment, and T-helper balance of lymphocytes between severe and mild asthma.

Applications

ICC

Species

Human

A S Abdulamir,R R Hafidh,F Abubakar,K A Abbas

Immunogenetics 29:202-5 PubMed2466778

1989

Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression.

Applications

Unspecified application

Species

Unspecified reactive species

V Bazil,I Hilgert,H Kristofová,D Maurer,V Horejsí

Folia biologica 35:289-97 PubMed2534480

1989

Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.

Applications

Unspecified application

Species

Unspecified reactive species

V Bazil,I Stefanová,I Hilgert,H Kristofová,S Vanĕk,A Bukovský,V Horejsí

View all publications

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Anti-CD45RA 抗体 [MEM-56]