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Immunology Adaptive Immunity B Cells CD
重组

重组Anti-CD45RA抗体[4KB5] (ab755)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (2)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [4KB5] (ab755)
  • Flow Cytometry - Anti-CD45RA antibody [4KB5] (ab755)
  • Anti-CD45RA antibody [4KB5] (ab755)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [4KB5] to CD45RA
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

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概述

  • 产品名称

    Anti-CD45RA抗体[4KB5]
    参阅全部 CD45RA 一抗
  • 描述

    小鼠单克隆抗体[4KB5] to CD45RA
  • 宿主

    Mouse
  • 经测试应用

    适用于: IHC-P, Flow Cytmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Tissue, cells or virus corresponding to CD45RA. B cell lymphoma

  • 阳性对照

    • IHC-P: Human tonsil tissue. Flow Cyt: Human peripheral blood mononuclear cells.
  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    4KB5
  • 同种型

    IgG1
  • 研究领域

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage

相关产品

  • Alternative Versions

    • Anti-CD45RA antibody [4KB5] - BSA and Azide free (ab256137)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab755于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use a concentration of 0.239 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use a concentration of 1.017 µg/ml.
说明
IHC-P
Use a concentration of 0.239 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use a concentration of 1.017 µg/ml.

靶标

  • 功能

    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
  • 疾病相关

    Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
    Multiple sclerosis
  • 序列相似性

    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • 结构域

    The first PTPase domain interacts with SKAP1.
  • 翻译后修饰

    Heavily N- and O-glycosylated.
  • 细胞定位

    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Target information above from: UniProt accession P08575 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 5788 Human
    • Omim: 151460 Human
    • SwissProt: P08575 Human
    • Unigene: 654514 Human
    • 别名

      • B220 antibody
      • CD45 antibody
      • CD45 antigen antibody
      • CD45R antibody
      • EC 3.1.3.48 antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • Loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lymphocyte antigen 5 antibody
      • Lyt-4 antibody
      • Protein tyrosine phosphatase, receptor type C antibody
      • Protein tyrosine phosphatase, receptor type, C antibody
      • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
      • Ptprc antibody
      • PTPRC_HUMAN antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • RT7 antibody
      • T200 antibody
      • T200 glycoprotein antibody
      • T200 leukocyte common antigen antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [4KB5] (ab755)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [4KB5] (ab755)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD45RA with ab755 at 0.239µg/ml, followed by ready to use secondary. Positive staining on human tonsil tissue is observed. The section was incubated with ab755 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Flow Cytometry - Anti-CD45RA antibody [4KB5] (ab755)
      Flow Cytometry - Anti-CD45RA antibody [4KB5] (ab755)

      Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) (right) labeling CD45RA with ab755 at 1.017µg/ml compared with mouse monoclonal IgG Isotype Control (left). Goat anti-mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

    • Anti-CD45RA antibody [4KB5] (ab755)
      Anti-CD45RA antibody [4KB5] (ab755)

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (2)

    发表研究结果有使用 ab755?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab755 被引用在 2 文献中.

    • Yang W  et al. ACE2 Correlated With Immune Infiltration Serves As A Novel Prognostic Biomarker In Clear Cell Renal Cell Carcinoma: Implication For COVID-19. Int J Biol Sci 17:20-31 (2021). PubMed: 33390830
    • Li FJ  et al. A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood 105:3295-302 (2005). PubMed: 15626731

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    ab755 - This antibody is a tissue culture supernatant but it has a concentration, can you tell me how you got this concentration? How do you know that it's accurate?   ab8260 what is the concentration of this product?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 25 2011

    Answer

    The concentration of the current lot of ab8260 that we have in stock is 2.0 mg/ml. However, they also informed me that this concentration does vary from lot to lot. Additionally, they have also let me know that the way the concentration was determined for ab755 was through radio immunoassay. 

    Read More

    Abcam Scientific Support

    回复于 Oct 25 2011

    Question

    No problem, only a question; the ds says: use at a dilution of 1 / 10 - 1 / 25 for 30 - 60 mins (ABC method). What is this referring to (esp. the incubation time), The customer is using the DAKO ABC kit.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 12 2004

    Answer

    Thank you for your enquiry. Ab755 has been tested for application in Immunohistochemistry, both formalin-fixed paraffin-embedded sections and frozen sections. It is recommended to use this antibody at a dilution of 1:10 - 1:25 in an ABC method and an incubation period or 30 minutes at room temperature is suggested. We have ABC Immunohistochemical Staining IHC protocols located in the protocol section of our website as well if your customer would like to refer to them.

    Read More

    Abcam Scientific Support

    回复于 Oct 12 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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