重组Anti-CD45RA抗体[4KB5] (ab755)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [4KB5] (ab755)](https://www.abcam.cn/ps/products/0/ab755/Images/ab755-368589-AntiCD45RA-antibody-4KB5-immunohistochemistry-tonsil-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [4KB5] (ab755)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [4KB5] to CD45RA
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD45RA抗体[4KB5]
参阅全部 CD45RA 一抗 -
描述
小鼠单克隆抗体[4KB5] to CD45RA -
宿主
Mouse -
经测试应用
适用于: IHC-P, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to CD45RA. B cell lymphoma
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阳性对照
- IHC-P: Human tonsil tissue. Flow Cyt: Human peripheral blood mononuclear cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
4KB5 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 0.239 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| Flow Cyt |
Use a concentration of 1.017 µg/ml.
|
| 说明 |
|---|
|
IHC-P
Use a concentration of 0.239 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
Flow Cyt
Use a concentration of 1.017 µg/ml. |
靶标
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功能
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. -
疾病相关
Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
Multiple sclerosis -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
结构域
The first PTPase domain interacts with SKAP1. -
翻译后修饰
Heavily N- and O-glycosylated. -
细胞定位
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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数据库链接
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
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别名
- B220 antibody
- CD45 antibody
- CD45 antigen antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [4KB5] (ab755)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD45RA with ab755 at 0.239µg/ml, followed by ready to use secondary. Positive staining on human tonsil tissue is observed. The section was incubated with ab755 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Flow Cytometry - Anti-CD45RA antibody [4KB5] (ab755)](https://www.abcam.cn/ps/products/0/ab755/Images/ab755-368590-Mouse-monoclonal-4KB5-to-CD45RA-flow-cytometry-human-peripheral-blood-mononuclear-cells.jpg)
Flow Cytometry - Anti-CD45RA antibody [4KB5] (ab755)Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) (right) labeling CD45RA with ab755 at 1.017µg/ml compared with mouse monoclonal IgG Isotype Control (left). Goat anti-mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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![Anti-CD45RA antibody [4KB5] (ab755)](https://www.abcam.cn/ps/products/0/ab755/Images/ab755-5-benefits-of-recombinant-antibodies.png)
Anti-CD45RA antibody [4KB5] (ab755)
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab755 被引用在 4 文献中.
- Yang W et al. ACE2 Correlated With Immune Infiltration Serves As A Novel Prognostic Biomarker In Clear Cell Renal Cell Carcinoma: Implication For COVID-19. Int J Biol Sci 17:20-31 (2021). PubMed: 33390830
- He Y et al. Molecular predictors of response to pembrolizumab in thymic carcinoma. Cell Rep Med 2:100392 (2021). PubMed: 34622229
- Yang B et al. The Impact of Immune Microenvironment on the Prognosis of Pancreatic Ductal Adenocarcinoma Based on Multi-Omics Analysis. Front Immunol 12:769047 (2021). PubMed: 34777388
- Li FJ et al. A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood 105:3295-302 (2005). PubMed: 15626731