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AB25078

Anti-CD45.1抗体[A20]

Anti-CD45.1 antibody [A20]

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(6 Publications)

Anti-CD45.1 antibody [A20] (ab25078) is a mouse monoclonal antibody detecting CD45.1 in Flow Cytometry, IP, ICC/IF. Suitable for Mouse.

- Trusted since 2005

查看别名

CD45, Ly-5, Ptprc, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, Lymphocyte antigen 5, T200, L-CA

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Flow Cytometry - Anti-CD45.1 antibody [A20] (AB25078)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD45.1 antibody [A20] (AB25078)

Flow Cytometry analysis of SJL mouse splenocytes labeling CD45.1 with ab25078 at 0.1 μg/106 cells (Purple). A Goat Anti-Mouse IgG2a, Human ads-AF647 was used as the secondary antibody. Grey - Isotype Control, Mouse IgG2a-UNLB followed by Goat Anti-Mouse IgG2a, Human ads-AF647.

不同偶联物与剂型 (6)

  • 667 Cy5®

    Cy5 ® Anti-CD45.1 antibody [A20]

  • 660 APC

    APC Anti-CD45.1 antibody [A20]

  • 519 FITC

    FITC Anti-CD45.1 antibody [A20]

  • Biotin

    Biotin Anti-CD45.1 antibody [A20]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD45.1 antibody [A20]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD45.1 antibody [A20]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

A20

亚型

IgG2a

轻链类型

kappa

不含载体蛋白

No

反应种属

Mouse

应用

IHC-FoFr, Flow Cyt, IP, ICC/IF

applications

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
存储溶液
pH: 8.2 Constituents: 100% Borate buffered saline
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD45.1 also known as Leukocyte Common Antigen (LCA) or Protein Tyrosine Phosphatase Receptor Type C (PTPRC) is a protein expressed on the surface of all nucleated hematopoietic cells. It has a molecular mass of approximately 180-220 kDa. This protein acts as a receptor-type protein tyrosine phosphatase. CD45.1 is an essential marker in the immune system particularly found on T cells B cells and other white blood cells. The expression of CD45.1 is seen across leukocytes where it plays a significant role in cell signaling and antigen receptor signaling.
Biological function summary

CD45.1 influences the immune response by modulating signals through antigen receptors. It operates as a single-pass type I membrane protein and is not known to be part of a larger complex. This protein is important in the activation and regulation of T-cells and B-cells by fine-tuning the threshold of signals required for cell activation. The presence of CD45.1 affects multiple processes in adaptive immunity impacting lymphocyte development and their further activation in immune responses.

Pathways

CD45.1 is integral in lymphocyte activation pathways. It influences the T-cell receptor (TCR) and B-cell receptor (BCR) signaling pathways by dephosphorylating specific kinases like Lck and Fyn which are important in initiating TCR and BCR signaling cascades. Moreover CD45.1 interacts with other proteins such as CD3 and CD19 being essential in the regulation of immune response and maintaining proper signaling amplitudes to ensure effective immune function.

CD45.1 is intricately linked to autoimmune diseases and leukemia. The protein's dysregulation can lead to improper immune signaling contributing to conditions such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). In leukemia the altered expression of CD45.1 and related proteins such as CD20 often correlates with disease progression and treatment response. Understanding the roles of CD45.1 can facilitate the development of targeted therapies in these contexts.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity.
See full target information Ptprc
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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular gastroenterology and hepatology 15:613-632 PubMed36436756

2022

Klebsiella pneumoniae Induces Inflammatory Bowel Disease Through Caspase-11-Mediated IL18 in the Gut Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qianjin Zhang,Xiaomin Su,Chunze Zhang,Wei Chen,Ya Wang,Xiaorong Yang,Dan Liu,Yuan Zhang,Rongcun Yang

Experimental and therapeutic medicine 20:375-384 PubMed32509014

2020

Modulation of the secretion of mesenchymal stem cell immunoregulatory factors by hydrolyzed fish collagen.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Liu,Jiao Sun

Oncoimmunology 5:e1211218 PubMed27757303

2016

Efficient tumor regression by adoptively transferred CEA-specific CAR-T cells associated with symptoms of mild cytokine release syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Linan Wang,Ning Ma,Sachiko Okamoto,Yasunori Amaishi,Eiichi Sato,Naohiro Seo,Junichi Mineno,Kazutoh Takesako,Takuma Kato,Hiroshi Shiku

EMBO molecular medicine 5:471-81 PubMed23417922

2013

Distinct functions of chemokine receptor axes in the atherogenic mobilization and recruitment of classical monocytes.

Applications

IHC-Fr

Species

Mouse

Oliver Soehnlein,Maik Drechsler,Yvonne Döring,Dirk Lievens,Helene Hartwig,Klaus Kemmerich,Almudena Ortega-Gómez,Manuela Mandl,Santosh Vijayan,Delia Projahn,Christoph D Garlichs,Rory R Koenen,Mihail Hristov,Esther Lutgens,Alma Zernecke,Christian Weber

PloS one 4:e5728 PubMed19478957

2009

A non-cytosolic protein of Trypanosoma evansi induces CD45-dependent lymphocyte death.

Applications

Unspecified application

Species

Mouse

Nicolas Antoine-Moussiaux,Anne Cornet,François Cornet,Stéphanie Glineur,Martin Dermine,Daniel Desmecht

Journal of virology 81:9801-11 PubMed17626103

2007

CD40-CD40 ligand interactions promote trafficking of CD8+ T cells into the brain and protection against West Nile virus encephalitis.

Applications

IHC-FoFr

Species

Mouse

Elizabeth Sitati,Erin E McCandless,Robyn S Klein,Michael S Diamond
View all publications
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