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重组RabMAb

重组Anti-CD45抗体[EPR28934-536] - BSA and Azide free (ab317447)

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Western blot - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
  • Western blot - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
  • Flow Cytometry - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
  • Flow Cytometry - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
  • Flow Cytometry - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
  • Immunoprecipitation - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
  • Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR28934-536] to CD45 - BSA and Azide free
  • Suitable for: ICC/IF, WB, Flow Cyt, IP, IHC-P
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-CD45抗体[EPR28934-536] - BSA and Azide free
    参阅全部 CD45 一抗

  • 描述

    兔单克隆抗体[EPR28934-536] to CD45 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, WB, Flow Cyt, IP, IHC-Pmore details

  • 种属反应性

    与反应: Mouse
    不与反应: Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: J774A.1, Mouse thymus lysates. IHC-P: Mouse spleen tissues. ICC: J774A.1 and Mouse PBMC cells. Flow Cyt: RAW 264.7, J774A.1 and Mouse PBMC cells. IP: J774A.1 cell.

  • 常规说明

    ab317447 is the carrier-free version of ab317446.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317447于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 147 kDa.
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 147 kDa.
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 功能

    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

  • 疾病相关

    Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.

  • 序列相似性

    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.

  • 结构域

    The first PTPase domain interacts with SKAP1.

  • 翻译后修饰

    Heavily N- and O-glycosylated.

  • 细胞定位

    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt

  • 数据库链接

    • 别名

      • B220 antibody
      • CD 45 antibody
      • CD45 antibody
      • CD45 antigen antibody
      • CD45R antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lyt-4 antibody
      • OTTHUMP00000033813 antibody
      • OTTHUMP00000033816 antibody
      • OTTHUMP00000033817 antibody
      • OTTHUMP00000038574 antibody
      • Protein tyrosine phosphatase receptor type c polypeptide antibody
      • Protein tyrosine phosphatase, receptor type C antibody
      • protein tyrosine phosphatase, receptor type, C antibody
      • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
      • Ptprc antibody
      • PTPRC_HUMAN antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • T200 antibody
      • T200 glycoprotein antibody
      • T200 leukocyte common antigen antibody
      see all

    图片

    • Western blot - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
      Western blot - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
      All lanes : Anti-CD45 antibody [EPR28934-536] (ab317446) at 1/1000 dilution

      Lane 1 : J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) whole cell lysate
      Lane 2 : C2C12 (mouse myoblast) whole cell lysate
      Lane 3 : HL-1 (mouse atrial muscle cell) whole cell lysate

      Lysates/proteins at 80 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 147 kDa
      Observed band size: 200 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab317446, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Low expression: C2C12, HL-1.

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 37917373).

      In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

    • Western blot - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
      Western blot - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
      All lanes : Anti-CD45 antibody [EPR28934-536] (ab317446) at 1/1000 dilution

      Lane 1 : Mouse thymus tissue lysate
      Lane 2 : Mouse skeletal muscle tissue lysate

      Lysates/proteins at 80 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 147 kDa
      Observed band size: 200 kDa why is the actual band size different from the predicted?


      Exposure time: 81 seconds


      This data was developed using ab317446, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Low expression: skeletal muscle.

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 37917373).

      In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)

      This data was developed using ab317446, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD45 with ab317446 at 1/2000 (0.258 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on mouse spleen.
      The section was incubated with ab317446 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)

      This data was developed using ab317446, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling CD45 with ab317446 at 1/2000 (0.258 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Low expression tissue: Low expression on immune cells of mouse skeletal muscle.
      The section was incubated with ab317446 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
      Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)

      This data was developed using ab317446, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) cells labelling CD45 with ab317446 at 1/500 (1.03 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).

      Confocal image showing membrane staining in J774A.1 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
      Low expression: C2C12
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
      Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)

      This data was developed using ab317446, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse PBMC (mouse peripheral blood mononuclear cell) cells labelling CD45 with ab317446 at 1/500 (1.03 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).

      Confocal image showing membrane staining in mouse PBMC (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Anti-rat CD3 mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

    • Flow Cytometry - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
      Flow Cytometry - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)

      This data was developed using ab317446, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) (Right) / C2C12 (mouse myoblast) (Left) cells labelling CD45 with ab317446 at 1/500 dilution (0.1ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Low expression: C2C12. Gated on viable cells.

    • Flow Cytometry - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
      Flow Cytometry - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)

      This data was developed using ab317446, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) (Right) / HL-1 (mouse atrial muscle cell) (Left) cells labelling CD45 with ab317446 at 1/500 dilution (0.1ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Low expression: HL-1. Gated on vaible cells.

    • Flow Cytometry - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
      Flow Cytometry - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)

      This data was developed using ab317446, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Mouse PBMC cells labelling CD45 with ab317446 at 1/500 dilution (0.1ug) / right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Cells were co-stained with anti-CD3 conjugated to Alexa Fluor® 647.
      Gated on viable cells.

    • Immunoprecipitation - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
      Immunoprecipitation - Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)

      This data was developed using ab317446, the same antibody clone in a different buffer formulation.

      CD45 was immunoprecipitated from 0.35 mg J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) whole cell lysate with ab317446 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317446 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) whole cell lysate
      Lane 2: ab317446 IP in J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) whole cell lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317446 in J774A.1 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 180 seconds.

    • Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)
      Anti-CD45 antibody [EPR28934-536] - BSA and Azide free (ab317447)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab317447？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab317447 尚未被引用在任何文献中。

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