Name: Anti-CD44 antibody [F10-44-2]
SKU: ab6124
Rating: 5 (5 reviews)

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Flow Cytometry - Anti-CD44 antibody [F10-44-2] (ab6124)

Key features and details

Mouse monoclonal [F10-44-2] to CD44
Suitable for: Flow Cyt, ICC/IF, IHC-P
Reacts with: Human
Isotype: IgG2a

FITC

产品名称

Anti-CD44抗体[F10-44-2]
参阅全部 CD44 一抗
描述

小鼠单克隆抗体[F10-44-2] to CD44
宿主

Mouse
经测试应用

适用于: Flow Cyt, ICC/IF, IHC-Pmore details
种属反应性

与反应: Human
免疫原

Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- In Flow Cytometry, this antibody gave a positive signal in peripheral blood lymphocytes. In IHC, this antibody gave a positive signal in human kidney carcinoma sections. ICC/IF: A431 cell line.
常规说明

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
纯度

Protein G purified
克隆

单克隆
克隆编号

F10-44-2
同种型

IgG2a
研究领域

Alternative Versions
- FITC Anti-CD44 antibody [F10-44-2] (ab30405)
Compatible Secondaries
Isotype control
- Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
Recombinant Protein
- Recombinant human CD44 protein (Active) (ab173996)

The Abpromise guarantee

Abpromise™承诺保证使用ab6124于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Flow Cyt		Use 0.5-1µg for 10⁶ cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF		Use a concentration of 5 µg/ml.
IHC-P	(3)	Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

说明
Flow Cyt Use 0.5-1µg for 10⁶ cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

功能

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
组织特异性

Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
序列相似性

Contains 1 Link domain.
结构域

The lectin-like LINK domain is responsible for hyaluronan binding.
翻译后修饰

Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
细胞定位

Membrane.
Target information above from: UniProt accession P16070 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 960 Human
- Omim: 107269 Human
- SwissProt: P16070 Human
- Unigene: 502328 Human
别名
- LHR antibody
- BA-1 antibody
- CD 44 antibody
- CD44 antibody
- CD44 antigen antibody
- CD44 molecule (Indian blood group) antibody
- CD44 molecule antibody
- CD44_HUMAN antibody
- CDw44 antibody
- CDW44 antigen antibody
- Cell surface glycoprotein CD44 antibody
- chondroitin sulfate proteoglycan 8 antibody
- CSPG8 antibody
- ECMR-III antibody
- Epican antibody
- Extracellular matrix receptor III antibody
- GP90 lymphocyte homing/adhesion receptor antibody
- HCELL antibody
- hematopoietic cell E- and L-selectin ligand antibody
- Heparan sulfate proteoglycan antibody
- Hermes antigen antibody
- homing function and Indian blood group system antibody
- HSA antibody
- HUTCH-I antibody
- HUTCH1 antibody
- HUTCHI antibody
- Hyaluronate receptor antibody
- IN antibody
- INLU-related p80 Glycoprotein antibody
- MC56 antibody
- MDU2 antibody
- MDU3 antibody
- MGC10468 antibody
- MIC4 antibody
- MUTCH I antibody
- MUTCH1 antibody
- PGP-1 antibody
- PGP-I antibody
- PGP1 antibody
- Phagocytic glycoprotein 1 antibody
- Phagocytic glycoprotein I antibody
- Soluble CD44 antibody
see all

Flow Cytometry - Anti-CD44 antibody [F10-44-2] (ab6124)

Overlay histogram showing peripheral blood lymphocytes stained with ab6124 (red line). The cells were incubated with the antibody (ab6124, 0.5µg/1x10⁶ cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/200 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (ab6124)Image from Le Bras GF et al., PLoS One. 2011;6(11):e27063. Epub 2011 Nov 1. Fig 3.; doi:10.1371/journal.pone.0027063; November 1, 2011, PLoS ONE 6(11): e27063.

Immunohistochemical analysis of Human esophogeal keratinocytes, staining CD44 with ab6124.

Keratinocytes were cultured in vivo to form an epithelium for 15 days before fixing in formaldehyde and embedding in paraffin. Primary antibody was incubated overnight at 4°C and secondary antibody for 30 minutes at 37°C. Staining was detected using DAB.
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [F10-44-2] (ab6124)

ab6124 staining CD44 in A431 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab6124 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (ab6124)

IHC image of CD44 staining in human kidney carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6124, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab6124？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6124 被引用在 65 文献中.

Chen R et al. Triple Cross-Linked Hyaluronic Acid Based on Tri-Hyal Technique Has More Durable Effect on Dermal Renewal. Clin Cosmet Investig Dermatol 15:691-701 (2022). PubMed: 35450398
Hopf A et al. Optimized Decellularization Protocol for Large Peripheral Nerve Segments: Towards Personalized Nerve Bioengineering. Bioengineering (Basel) 9:N/A (2022). PubMed: 36134958
Kim H et al. Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin. Nat Commun 13:6581 (2022). PubMed: 36323693
Marrelli D et al. Immunohistochemical Markers of the Epithelial-to-Mesenchymal Transition (EMT) Are Related to Extensive Lymph Nodal Spread, Peritoneal Dissemination, and Poor Prognosis in the Microsatellite-Stable Diffuse Histotype of Gastric Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 36551509
Padmashri R et al. Modeling human-specific interlaminar astrocytes in the mouse cerebral cortex. J Comp Neurol 529:802-810 (2021). PubMed: 32639590

View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A

提交评价提交问题

1-10 of 12 Abreviews or Q&A

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (Skin)

Permeabilization

Specification

Skin

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ahmar Aziz

Verified customer

提交于 Jul 08 2016

Application

Western blot

Sample

Human Cell lysate - whole cell (BEL 7404)

Gel Running Conditions

Non-reduced Denaturing (10%)

Loading amount

40 µg

Treatment

80 µM Doxorubicin for 8hrs/24hrs

Specification

BEL 7404

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. guoqing zhu

Verified customer

提交于 Jan 22 2016

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (urinary bladder)

Specification

urinary bladder

Fixative

Formaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: EDTA

Permeabilization

Yes - Triton X100

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. whan kim

Verified customer

提交于 May 18 2009

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Skin tumor)

Specification

Skin tumor

Fixative

Formaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric buffer

Permeabilization

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 27 2009

Question

Customer inquiry regarding 5 antibodies in the Human Mesenchymal Stromal Cell Marker panel:
CD44 (ab6124)
CD45 (ab10559)
CD90 (ab23894)
CD105 (ab44967)
CD29 (ab52971)

Customer wondering which antibodies are positive mesenchymal stromal cell markers and which are negative. Also wondering what flow cytometry procedure is in terms of gating and the expected color associated with each antibody.

Abcam community

Verified customer

Asked on Nov 29 2012

Answer

Thank you for contacting Abcam.
Earlier today we discussed the Human Mesenchymal Stromal Cell Marker panel ab93758. Based on the information on the datasheet, I compiled the following information for you:
1. Positive selection (CD105, CD29, CD44, CD90)
2. Negative selection (CD45)
The stem cell panel ab93758 consists of 5 unconjugated primary antibodies. These antibodies can be used with the secondary antibodies against mouse or rabbit of your choice. You can therefore adapt the fluorochrome choice to the ability of your machine.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Abcam Scientific Support

回复于 Nov 30 2012

Question

I would like to know whether ab6124 is able to react against CD44s and CD44v molecule on liver cancer cells.

Abcam community

Verified customer

Asked on Jul 30 2012

Answer

Thank you for contacting us.

Based on the information from the journal Jpn J. Cancer Res 89 283-90 March 1989 (Yutaka Tokue et. al.), it states that this monoclonal antibody can recognises CD44s. Although the experiment sample used in this study was breast tumour cells but I believe the molecule should be the same in liver cells.

I hope this information is helpful to you. Please do not hesitate to contact us should you need any further information.

Abcam Scientific Support

回复于 Jul 30 2012

Question

How was your antiCD44 purified, was this done by ion exchange or using a different method? Seeing how the product datasheet is a bit unclear about this.

Waiting for your reply and thank you for your kind cooperation.

Abcam community

Verified customer

Asked on Jun 06 2012

Answer

Thank you for contacting Abcam regarding ab6124.

This ab was affinity purified on a protein G column. Please let me know if this information is sufficient or if you require additional details.

I hope this information is helpful. Do not hesitate to contact me if you have any additional questions or concerns.

Abcam Scientific Support

回复于 Jun 06 2012

Question

Hi,
a customer of ours is interested in your ab93758. She has co-coltures of stromal mesenchymal stem cellsand T lymphocytes isolated from bone.
She would like to detect only stromal mesenchymal stem, but not T lymphocytes. Can you please let me know if among the markers (CD44, CD45, CD90 CD29 and CD105) included within ab93758, there is some marker able to detect also T lymphocytes?

Thanks in advance and kind regards

Abcam community

Verified customer

Asked on May 31 2012

Answer

Thank you for your enquiry and interest in our products.

I can confirm that this panel contains 5 primary antibodies which recognize human mesenchymal stromal cells: ab6124, ab10559, ab23894, ab44967, ab52971. The datasheets can be found at these sites:

https://www.abcam.com/cd44-antibody-f10-44-2-ab6124.html

https://www.abcam.com/cd45-antibody-ab10559.html

https://www.abcam.com/cd90--thy1-antibody-af-9-ab23894.html

https://www.abcam.com/cd105-antibody-105c02-ab44967.html

https://www.abcam.com/integrin-beta-1-antibody-ep1041y-carboxyterminal-end-ab52971.html

There are some very informative and useful websites summarizing the different CD markers. You may wish to recommend to your customer for further consideration:

https://docs.abcam.com/pdf/immunology/cdantigen_poster.pdf

http://pathologyoutlines.com/cdmarkers.html

If you need any further assistance in the future, please do not hesitate to contact me.

Abcam Scientific Support

回复于 May 31 2012

Question

Product code: 93574
Inquiry: Our customer is looking for the following : CD44 with the clone H90" . The item we offered against his request is AB93574 which include Clone F10-44-2 not clone 'H90'. Kindly advice which Item from ABCAM matches his request. Thank you

Abcam community

Verified customer

Asked on May 15 2012

Answer

Thank you for contacting us.

Unfortunately, we do not have an anti-CD44 antibody from the H90 clone in our catalogue. We do however have several other antibodies against this target which may be suitable for your customer. The catalogue number you mentioned (ab93574) does not match to an anti-CD44 antibody. The clone mouse monoclonal anti-CD44 clone F10-44-2 is available with many different conjugates which can be viewed from the following link:

https://www.abcam.com/index.html?pageconfig=searchresults&search=cd44&pt=1&sk=clonenum&sv=F10-44-2&sn=F10-44-2&l=3&fViewMore=1

The suitability of this clone will depend on the application and samples your customer intends to use. If you could give me a few more details about this I may be able to advise further as to whether this clone would be suitable or if one of our other anti-CD44 antibodies would be more appropriate.

I hope this information has been of some help. If you would like any further assistance please do let me know.

Abcam Scientific Support

回复于 May 15 2012

Question

Here you are my answers to abcam in details regarding my project so please forward to them and hopfuly I can get their guide asap. I am isolating human hertwig's epithelial root sheath (HERS) cells from periodontal ligament (PDL). So the cells that I am isolating are epithelial that should express these two epithelial markers as published in the literature (E-cadherin and Pan-cytokeratin) and will compare them with Stem cells from Human exfoliated deciduous teeth (SHED) using another two anibodies (CD44) that should be highly expressed in SHED than HERS and (CD73) that will be expressed similarly in both cells. So for characterization I ordered these antibodies from abcam: 1- Mouse monoclonal (HECD-1) to E Cadherin (ab1416) 2- Mouse monoclonal (PCK-26) to pan Cytokeratin (ab6401) 3- Mouse monoclonal (F10-44-2) to CD44 (ab6124) 4- Mouse monoclonal (7G2) to CD73 (ab54217) - Immunofluorescence staining steps (first time) HERS were cultured in 4 well culture slide and the second day we did the following: a- Discard the medium b- Wash 3 times with PBS each time two mins c- Add 500 µL methanol to each well for 20 mins at 4°C d- Discard methanol and wash 3 times with PBS each time two mins e- Add 7 drops of blocking reagent (from Millipore/ comes in the kit of immunoperoxidase secondary detection system) keep it at room temperature for 1 hr. f- Discard blocking reagent and add the primary antibodies (1:50 ratio) (each antibody was applied separately in each well of the same chamber slide) (we diluted the antibodies using PBS) g- Keep the chamber slide in 4°C for overnight. h- Then discard the antibodies, wash 3 times with PBS each time two mins i- Add 500 µL of secondary antibody (anti mouse FITC) to each well and keep it for 1 hr at room temperature j- Discard secondary antibody and wash 3 times with PBS each time two mins k- Add 500 µL of DAPI to each well keep it for 1 hr in a dark room l- Discard DAPI and wash properly with PBS m- The cover of the chamber slide was chopped and fluorescence mounting medium was applied (from Dako: S302380) n- Cover slip was placed and the observed under fluorescence microscope image analyzer Under microscope: We observed the following (as I sent you previously some images); Green cells with green background and blues nucleus. So how to get black background with only green cells and blue nucleus as we got used for immunofluoescence images? - We did optimize the method (second time) using the same previous procedures but with primary antibodies 1:300 but we got the same result. - We did optimize the method (third time) using the same previous steps but with these changes: a- We used PBS with Tween-20 for proper washing b- Apply methanol and keep it in -20°C for 5 mins c- Primary antibodies were applied with ratio (1:500) and keep it for 9 hrs at 4°C d- DAPI was applied for half hr in a dark room. - But under microscope we got the same result. So we are planning to use primary antibodies 1:1000 and keep it for 3 hrs but we do really need your recommendation and help to get the correct result before we run the test. And waiting for your new quotation please as I did email you. Best regards,

Abcam community

Verified customer

Asked on Oct 31 2011

Answer

Thank you for contacting us. The background should be black. I am sorry we never had a question like this before. It looks like this problem might be due to instrumentation then antibodies itself. I have following recommendations that might help; - Use less number of cells per well. - Check the viability and passage level of cells. - The cells must be healthy with low number of passages. - Avoid drying out plate between washings. - Check microscope settings. - Check if the blocking is sufficiently done. You can also use 3-5% BSA for 1-2 hour in PBST for blocking. - Use more diluted primary antibodies. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam Scientific Support

回复于 Oct 31 2011

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

组织特异性

序列相似性

结构域

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (65)

客户评价及客户问答

Immunohistochemistry (Frozen sections) abreview for Anti-CD44 antibody [F10-44-2]

Western blot abreview for Anti-CD44 antibody [F10-44-2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD44 antibody [F10-44-2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD44 antibody [F10-44-2]