重组Anti-CD44抗体[BLR038F] (ab243894)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [BLR038F] (ab243894)](https://www.abcam.cn/ps/products/243/ab243894/Images/ab243894-334369-anti-cd44-antibody-blr038f-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [BLR038F] (ab243894)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR038F] to CD44
- Suitable for: Flow Cyt, ICC, IHC-P, IP, WB
- Knockout validated
- Reacts with: Mouse, Human
概述
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产品名称
Anti-CD44抗体[BLR038F]
参阅全部 CD44 一抗 -
描述
兔单克隆抗体[BLR038F] to CD44 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt, ICC, IHC-P, IP, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human CD44 aa 700 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_000601.3 and Gene ID 960.
Database link: P16070-1 -
阳性对照
- WB: 786-O, KG-1, HeLa and A549 cell lysates. EL4 and BW5147.3 cell lysates. IHC-P: Human head and neck squamous cell carcinoma tissue. ICC: HCT116 cells. Flow Cyt: HeLa cells.
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常规说明
This product is sold under License from Bethyl Laboratories, Inc.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
纯化说明
Recombinant antibody was purified from cell culture supernatant. -
克隆
单克隆 -
克隆编号
BLR038F -
同种型
IgG -
研究领域
相关产品
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab243894于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 1µl for 106 cells.
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| ICC |
1/100 - 1/500.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at an assay dependent concentration.
Use 20µl/mg lysate. |
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| WB |
1/1000.
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| 说明 |
|---|
|
Flow Cyt
Use 1µl for 106 cells. |
|
ICC
1/100 - 1/500. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IP
Use at an assay dependent concentration. Use 20µl/mg lysate. |
|
WB
1/1000. |
靶标
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功能
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
组织特异性
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
序列相似性
Contains 1 Link domain. -
结构域
The lectin-like LINK domain is responsible for hyaluronan binding. -
翻译后修饰
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 960 Human
- Entrez Gene: 12505 Mouse
- Omim: 107269 Human
- SwissProt: P16070 Human
- SwissProt: P15379 Mouse
- Unigene: 502328 Human
- Unigene: 423621 Mouse
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别名
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [BLR038F] (ab243894)Formalin-fixed, paraffin-embedded human head and neck squamous cell carcinoma tissue stained for CD44 using ab243894 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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![Western blot - Anti-CD44 antibody [BLR038F] (ab243894)](https://www.abcam.cn/ps/products/243/ab243894/Images/ab243894-400170-anti-cd44-antibody-blr038f-western-blot-hela-wt-hela-cd44-knockout-a549-lncap.jpg)
Western blot - Anti-CD44 antibody [BLR038F] (ab243894)All lanes : Anti-CD44 antibody [BLR038F] (ab243894) at 1/1000 dilution
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : CD44 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 4 : LNCaP (Human prostate cancer cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 80 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab243894 observed at 80 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab243894 was shown to react with CD44 in wild-type HeLa cells in western blot. Loss of signal was observed when CD44 knockout sample was used. Wild-type HeLa and CD44 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab243894 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-CD44 antibody [BLR038F] (ab243894)](https://www.abcam.cn/ps/products/243/ab243894/Images/ab243894-334372-anti-cd44-antibody-blr038f-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD44 antibody [BLR038F] (ab243894)CD44 was immunoprecipitated from 1.0 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab243894 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243894 at 1/1000 dilution.Lane 1: ab243894 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 seconds.
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![Flow Cytometry - Anti-CD44 antibody [BLR038F] (ab243894)](https://www.abcam.cn/ps/products/243/ab243894/Images/ab243894-464306-anti-cd44-antibody-flow-cytometry-hela-human.jpg)
Flow Cytometry - Anti-CD44 antibody [BLR038F] (ab243894)Flow Cytometry analysis of HeLa cells labeling CD44 with ab243894 (red). Cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG. -
![Immunocytochemistry - Anti-CD44 antibody [BLR038F] (ab243894)](https://www.abcam.cn/ps/products/243/ab243894/Images/ab243894-334370-anti-cd44-antibody-blr038f-immunocytochemistry.jpg)
Immunocytochemistry - Anti-CD44 antibody [BLR038F] (ab243894)Formalin-fixed, paraffin-embedded HCT 116 (human colorectal carcinoma cell line) cells labeling CD44 using ab243894 at 1/500 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining. -
![Western blot - Anti-CD44 antibody [BLR038F] (ab243894)](https://www.abcam.cn/ps/products/243/ab243894/Images/ab243894-334371-anti-cd44-antibody-blr038f-western-blot.jpg)
Western blot - Anti-CD44 antibody [BLR038F] (ab243894)All lanes : Anti-CD44 antibody [BLR038F] (ab243894) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : 786-O whole cell lysate
Lane 3 : HEK-293T whole cell lysate
Lane 4 : Jurkat-3 whole cell lysate
Lane 5 : A549 whole cell lysate
Lane 6 : MCF7 whole cell lysate
Lane 7 : HepG2 whole cell lysate
Lane 8 : KG-1 whole cell lysate
Lane 9 : RPMI 8226 whole cell lysate
Lane 10 : MOLT-4 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG antibodyDetection: chemiluminescence with an exposure time of 3 seconds.
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![Western blot - Anti-CD44 antibody [BLR038F] (ab243894)](https://www.abcam.cn/ps/products/243/ab243894/Images/ab243894-464310-anti-cd44-antibody-wester-blot-el4-mouse.jpg)
Western blot - Anti-CD44 antibody [BLR038F] (ab243894)All lanes : Anti-CD44 antibody [BLR038F] (ab243894) at 1/1000 dilution
Lane 1 : BW5147.3 cell lysate
Lane 2 : EL4 cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Exposure time: 10 secondsDetection method: Chemiluminescence
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![Anti-CD44 antibody [BLR038F] (ab243894)](https://www.abcam.cn/ps/products/243/ab243894/Images/ab243894-6-benefits-of-recombinant-antibodies.png)
Anti-CD44 antibody [BLR038F] (ab243894)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab243894 被引用在 1 文献中.
- Li S et al. Schisandrin B inhibits epithelial-mesenchymal transition and stemness of large-cell lung cancer cells and tumorigenesis in xenografts via inhibiting the NF-?B and p38 MAPK signaling pathways. Oncol Rep 45:N/A (2021). PubMed: 33907830