重组Anti-CD44抗体[1M7.8.1] (ab119348)
![Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (ab119348)](https://www.abcam.cn/ps/products/119/ab119348/Images/ab119348-444785-ab272229444774AntiCD44antibody1M781immunocytochemistrynih3t3mouse.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (ab119348)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [1M7.8.1] to CD44
- Suitable for: ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-CD44抗体[1M7.8.1]
参阅全部 CD44 一抗 -
描述
大鼠单克隆抗体[1M7.8.1] to CD44 -
宿主
Rat -
特异性
Detects a standard 85-kDa isoform of CD44 and a number of high molecular mass variant isoforms. -
经测试应用
适用于: ICC/IFmore details
不适用于: Flow Cyt,IHC-P or WB -
种属反应性
与反应: Mouse, Human -
免疫原
Full length protein corresponding to Mouse CD44.
Database link: P15379 -
阳性对照
- ICC/IF: HeLa and NIH/3T3 cells.
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常规说明
This product has switched from a hybridoma to recombinant production method on 02nd November 2020
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
1M7.8.1 -
同种型
IgG2b -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119348于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/250.
|
| 说明 |
|---|
|
ICC/IF
1/250. |
靶标
-
功能
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
组织特异性
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
序列相似性
Contains 1 Link domain. -
结构域
The lectin-like LINK domain is responsible for hyaluronan binding. -
翻译后修饰
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 960 Human
- Entrez Gene: 12505 Mouse
- Omim: 107269 Human
- SwissProt: P16070 Human
- SwissProt: P15379 Mouse
- Unigene: 502328 Human
- Unigene: 423621 Mouse
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别名
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (ab119348)
NIH/3T3 (Mouse embryo fibroblast cell line) cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab119348 at 1/250 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab119348 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Confocal image showing strong membranous staining in NIH/3T3 cells. -
![Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (ab119348)](https://www.abcam.cn/ps/products/119/ab119348/Images/ab119348-444784-antiCD44-antibodyICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (ab119348)HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab119348 at 1/250 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab119348 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Confocal image showing strong membranous staining in HeLa cells.
Low expression control: MCF7 (PMID: 23039365).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab119348 被引用在 23 文献中.
- Zha D et al. Proteomic Profiling of Fallopian Tube-Derived Extracellular Vesicles Using a Microfluidic Tissue-on-Chip System. Bioengineering (Basel) 10:N/A (2023). PubMed: 37106610
- Lin F et al. Mesenchymal stem cells protect against ferroptosis via exosome-mediated stabilization of SLC7A11 in acute liver injury. Cell Death Dis 13:271 (2022). PubMed: 35347117
- Yamasaki S et al. A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation. iScience 25:103657 (2022). PubMed: 35024589
- Kashirina A et al. FLIM for Evaluation of Difference in Metabolic Status between Native and Differentiated from iPSCs Dermal Papilla Cells. Cells 11:N/A (2022). PubMed: 36078136
- Assunção M et al. Hyaluronic acid drives mesenchymal stromal cell-derived extracellular matrix assembly by promoting fibronectin fibrillogenesis. J Mater Chem B 9:7205-7215 (2021). PubMed: 33710248