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重组RabMAb

重组Anti-CD43抗体[EPR28619-85] - BSA and Azide free (ab317314)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)
  • Flow Cytometry - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)
  • Flow Cytometry - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)
  • Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR28619-85] to CD43 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-CD43抗体[EPR28619-85] - BSA and Azide free
    参阅全部 CD43 一抗

  • 描述

    兔单克隆抗体[EPR28619-85] to CD43 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, IHC-P, Flow Cytmore details
    不适用于: IP or WB

  • 种属反应性

    与反应: Mouse
    不与反应: Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Mouse spleen tissue and EL4 cells. ICC/IF: mouse splenocyte cells. Flow Cyt: Mouse spleen cells.

  • 常规说明

    ab317314 is the carrier-free version of ab317313.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317314于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
说明
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
应用说明
Is unsuitable for IP or WB.

靶标

  • 功能

    One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.

  • 组织特异性

    Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

  • 翻译后修饰

    Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CD 43 antibody
      • CD43 antibody
      • CD43 antigen antibody
      • Galactoglycoprotein antibody
      • GALGP antibody
      • GPL 115 antibody
      • GPL115 antibody
      • Human gene for sialophorin antibody
      • Leucocyte sialoglycoprotein antibody
      • LEUK_HUMAN antibody
      • Leukocyte large sialoglycoprotein antibody
      • Leukocyte sialoglycoprotein antibody
      • Leukosialin antibody
      • LSN antibody
      • Ly-48 antibody
      • sialophorin (gpL115, leukosialin, CD43) antibody
      • Sialophorin antibody
      • Spn antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)

      This data was developed using ab317313, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD43 with ab317313 at 1/500 (1.052 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on T cells of mouse spleen.
      The section was incubated with ab317313 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)

      This data was developed using ab317313, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling CD43 with ab317313 at 1/500 (1.052 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on immune cells of mouse lung.
      The section was incubated with ab317313 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)

      This data was developed using ab317313, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded (A) EL4 (mouse lymphoma T lymphocyte). (B) C2C12 (mouse myoblast). tissue labeling CD43 with ab317313 at 1/100 (5.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on (A) EL4, no staining on (B) C2C12.
      The section was incubated with ab317313 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)

      This data was developed using ab317313, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling CD43 with ab317313 at 1/500 (1.052 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Negative control: No staining on mouse skeletal muscle.
      The section was incubated with ab317313 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)
      Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)

      This data was developed using ab317313, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse splenocyte cells labelling CD43 with ab317313 at 1/50 (10.52 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

      Confocal image showing membranous staining in subset of mouse splenocyte (shown in green), which is not co-localised with CD11b (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue).
      Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

      ab8878 Anti-CD11b Rat monoclonal antibody was used to counterstain at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

    • Flow Cytometry - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)
      Flow Cytometry - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)

      This data was developed using ab317313, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Mouse spleen cell cells labelling CD43 with ab317313 at 1/500 dilution (0.1ug)/right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Cells were co-stained with anti-CD4 conjugated to Alexa Fluor®647.
      Gated on viable cells.

    • Flow Cytometry - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)
      Flow Cytometry - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)

      This data was developed using ab317313, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Mouse spleen cell cells labelling CD43 with ab317313 at 1/500 dilution (0.1ug)/right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Cells were co-stained with anti-CD11b conjugated to Brilliant Violet 510.
      Gated on viable cells.

    • Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)
      Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (ab317314)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

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