AB134087

Anti-CD42b 抗体 [EPR6995]

Anti-CD42b antibody [EPR6995]

RabMAb
Recombinant
了解详情

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(14 Publications)

Anti-CD42b antibody [EPR6995] (ab134087) is a rabbit monoclonal antibody detecting CD42b in Western Blot, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

查看别名

CD42b, Platelet glycoprotein Ib alpha chain, GP-Ib alpha, GPIb-alpha, GPIbA, Glycoprotein Ibalpha, Antigen CD42b-alpha, GP1BA

5 Images

Unknown

Western blot - Anti-CD42b antibody [EPR6995] (AB134087)

All lanes:

Western blot - Anti-CD42b antibody [EPR6995] (ab134087) at 1/10000 dilution

All lanes:

Human platelet lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-rabbit at 1/2000 dilution

Predicted band size: 72 kDa

Observed band size: 143 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR6995] (AB134087)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR6995] (AB134087)

ab134087 staining CD42b in human bone marrow tissue by Immunohistochemistry (IHC-P - paraformaldehyde-fixed paraffin-embedded sections). Tissue was fixed with paraffin and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

Negative control 1 : PBS in place of primary antibody.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR6995] (AB134087)

Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling CD42b with ab134087 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Western blot - Anti-CD42b antibody [EPR6995] (AB134087)

Diluting and blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CD42b antibody [EPR6995] (ab134087) at 1/10000 dilution

All lanes:

Human platelets at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 72 kDa

false

OI-RD Scanning - Anti-CD42b antibody [EPR6995] (AB134087)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CD42b antibody [EPR6995] (AB134087)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (3)

Carrier free

Anti-CD42b antibody [EPR6995] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD42b antibody [EPR6995]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-CD42b antibody [EPR6995]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR6995

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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您可能对以下产品感兴趣:

AB309423

Human CD42b ELISA Kit

0 Reviews

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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

What is this antibody validated in?
Anti-CD42b antibody [EPR6995] (ab134087) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of CD42b?
Anti-CD42b [EPR6995] (ab134087) specifically detects a band for CD42b (UniProt: P07359) at a molecular weight of 69kDa.

Trusted by the scientific community
Anti-CD42b [EPR6995] (ab134087) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR6995] also available for your convenience: ab134087, Carrier free - ab221213, Carrier free - ab271901, Alexa Fluor® 568 - ab313098, Alexa Fluor® 647 - ab313595

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Stable for 12 months at -20°C

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium.

See full target information GP1BA

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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:14568 PubMed37666907

2023

Association of clot ultrastructure with clot perviousness in stroke patients.

Applications

Unspecified application

Species

Unspecified reactive species

Young Dae Kim,Il Kwon,Youngseon Park,Hyungwoo Lee,Il Hyung Lee,In Hwan Lim,Soon-Ho Hong,Hye Sun Lee,Hyo Suk Nam,Ji Hoe Heo

Journal of stroke 24:300-302 PubMed35677986

2022

Different Thrombus Histology in a Cancer Patient with Deep Vein Thrombosis and Recurrent Strokes.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Eun Sun,Han Kyu Na,Soomin Kwak,Young Dae Kim,Hyo Suk Nam,Ji Hoe Heo

Aging cell 20:e13358 PubMed33942972

2021

Platelet biomarkers for a descending cognitive function: A proteomic approach.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Yu,Yanchao Liu,Benrong He,Ting He,Chongyang Chen,Jiahua He,Xifei Yang,Jian-Zhi Wang

Journal of cellular physiology 236:6726-6741 PubMed33611789

2021

HCMV modulates c-Mpl/IEX-1 pathway-mediated megakaryo/thrombopoiesis via PDGFRα and αvβ3 receptors after allo-HSCT.

Applications

Unspecified application

Species

Unspecified reactive species

Fei-Er Feng,Gao-Chao Zhang,Feng-Qi Liu,Yun He,Xiao-Lu Zhu,Xiao Liu,Yu Wang,Jing-Zhi Wang,Hai-Xia Fu,Yu-Hong Chen,Wei Han,Ying-Jun Chang,Lan-Ping Xu,Kai-Yan Liu,Xiao-Jun Huang,Xiao-Hui Zhang

Fetal and pediatric pathology 40:387-394 PubMed31986954

2020

CD42b Immunostaining as a Marker for Placental Fibrinoid in Normal Pregnancy and Complications.

Applications

Unspecified application

Species

Unspecified reactive species

Peilin Zhang

Annals of neurology 86:143-149 PubMed31025392

2019

Histological features of intracranial thrombi in stroke patients with cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hyungjong Park,Jayoung Kim,Jimin Ha,In Gun Hwang,Tae-Jin Song,Joonsang Yoo,Seong Hwan Ahn,Kyoungsub Kim,Byung Moon Kim,Dong Joon Kim,Young Dae Kim,Hyo Suk Nam,Il Kwon,Hyun-Jung Choi,Sung-Il Sohn,Hye Sun Lee,Ji Hoe Heo

Oncology letters 16:6988-6997 PubMed30546432

2018

Clinical diagnosis of adult patients with acute megakaryocytic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Guangjie Zhao,Wanling Wu,Xiaoqin Wang,Jingwen Gu

Journal of stroke 19:367-369 PubMed29037008

2017

Histopathological Findings of Intracranial Thrombi in Nonbacterial Thrombotic Endocarditis.

Applications

Unspecified application

Species

Unspecified reactive species

Kyoungsub Kim,Jayoung Kim,Seong Hwan Ahn,Woo Seok Ha,Yu Jin Koo,Dong Joon Kim,Hyo Suk Nam,Ji Hoe Heo

BMC cancer 17:294 PubMed28449652

2017

Potential of extravasated platelet aggregation as a surrogate marker for overall survival in patients with advanced gastric cancer treated with preoperative docetaxel, cisplatin and S-1: a retrospective observational study.

Applications

IHC-P

Species

Human

Hiroto Saito,Sachio Fushida,Tomoharu Miyashita,Katsunobu Oyama,Takahisa Yamaguchi,Tomoya Tsukada,Jun Kinoshita,Hidehiro Tajima,Itasu Ninomiya,Tetsuo Ohta

Oncotarget 7:14983-5017 PubMed26934552

2016

Immunogenomics reveal molecular circuits of diclofenac induced liver injury in mice.

Applications

IHC

Species

Unspecified reactive species

Eun-Hee Lee,Jung-Hwa Oh,Saravanakumar Selvaraj,Se-Myo Park,Mi-Sun Choi,Reinhard Spanel,Seokjoo Yoon,Jürgen Borlak

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

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cd42b-antibody-epr6995-low-endotoxin-azide-free-ab221213

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CD42b 抗体 [EPR6995]

Anti-CD42b antibody [EPR6995]

Western blot - Anti-CD42b antibody [EPR6995] (AB134087)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR6995] (AB134087)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR6995] (AB134087)

Western blot - Anti-CD42b antibody [EPR6995] (AB134087)

OI-RD Scanning - Anti-CD42b antibody [EPR6995] (AB134087)

不同偶联物与剂型 (3)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

反应性数据

您可能对以下产品感兴趣:

Human CD42b ELISA Kit

为什么推荐这个？

产品详情

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存时间

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

产品实验方案

靶点信息

文献 (14)

Abcam Product Promise

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