重组Anti-CD40抗体[EPR18005-4]

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD40 antibody [EPR18005-4] (AB212058)

Flow cytometric analysis of A20 (mouse reticulum sarcoma cell line) cell line labeling CD40 with ab212058 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD40 antibody [EPR18005-4] (AB212058)

Immunofluorescent analysis of 100% methanol-fixed A20 (mouse reticulum sarcoma cell line) cells labeling CD40 with ab212058 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Membranous staining on A20 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

• WB

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Western blot - Anti-CD40 antibody [EPR18005-4] (AB212058)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular mass observed is consistent with the literature (PMID : 23404288 and PMID : 16709811).

All lanes:

Western blot - Anti-CD40 antibody [EPR18005-4] (ab212058) at 1/1000 dilution

All lanes:

A20 (mouse reticulum sarcoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 30 kDa

Observed band size: 42 kDa

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Exposure time: 3min