Anti-CD40抗体(ab13545)
Key features and details
- Rabbit polyclonal to CD40
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Human, Pig, Monkey
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CD40抗体
参阅全部 CD40 一抗 -
描述
兔多克隆抗体to CD40 -
宿主
Rabbit -
特异性
The antibody specificity is confirmed by specific peptide inhibition in immunoblotting studies. -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Human, Pig, Monkey
预测可用于: Chicken, Dog
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免疫原
Synthetic peptide:
CQPVTQEDGKESRISVQERQ
conjugated to KLH, corresponding to amino acids 258-277 of Human CD40. -
常规说明
Storage below -20°C is not recommended. For maximum product recovery centrifuge the product vial before removing cap. Shipped on gel packs.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Peptide affinity purified. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13545于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (5) |
Use at an assay dependent concentration.
|
| WB | (2) |
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
|
| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa). |
靶标
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功能
Receptor for TNFSF5/CD40LG. -
组织特异性
B-cells and in primary carcinomas. -
疾病相关
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. -
序列相似性
Contains 4 TNFR-Cys repeats. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 958 Human
- Entrez Gene: 21939 Mouse
- Entrez Gene: 171369 Rat
- Omim: 109535 Human
- SwissProt: P25942 Human
- SwissProt: P27512 Mouse
- Unigene: 472860 Human
- Unigene: 271833 Mouse
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别名
- AI326936 antibody
- B cell associated molecule CD40 antibody
- B cell surface antigen CD40 antibody
see all
图片
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Western blot - Anti-CD40 antibody (ab13545)All lanes : Anti-CD40 antibody (ab13545) at 1/200 dilution
Lane 1 : ESK4 cell lysate
Lane 2 : Molt 4 cell lysate
Predicted band size: 34 kDa -
Western blot - Anti-CD40 antibody (ab13545)This image is courtesy of an anonymous AbreviewAll lanes : Anti-CD40 antibody (ab13545) at 1/5000 dilution
Lane 1 : Human B chronic lymphocytic leukaemia cells, nuclear fraction
Lane 2 : Human B chronic lymphocytic leukaemia cells, cytoskeletal fraction
Lane 3 : Human B chronic lymphocytic leukaemia cells, membrane fraction
Lane 4 : Human B chronic lymphocytic leukaemia cells, cytosolic fraction
Lysates/proteins at 18 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe primary antibody was incubated with the membrane for 16 hours at 4°C.
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Western blot - Anti-CD40 antibody (ab13545)Anti-CD40 antibody (ab13545) at 1/1000 dilution +Recombinant human CD40 protein (Fc Chimera Active) (ab83917) at 1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Exposure time: 8 minutes -
Western blot - Anti-CD40 antibody (ab13545)All lanes : Anti-CD40 antibody (ab13545) at 1/1000 dilution
Lanes 1-2 : Control human HCAEC cells - whole cell lysate
Lanes 3-4 : Treated human HCAEC cells - whole cell lysate
Secondary
All lanes : HRP conjugated Goat Anti-Rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 20 seconds
This image is courtesy of an Abreview submitted on 10 August 2005.
数据表及文件
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SDS download
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Datasheet download
文献 (27)
ab13545 被引用在 27 文献中.
- Abu El-Asrar AM et al. CD40 Ligand-CD40 Interaction Is an Intermediary between Inflammation and Angiogenesis in Proliferative Diabetic Retinopathy. Int J Mol Sci 24:N/A (2023). PubMed: 37958563
- Yan C et al. Generation of Orthotopic Patient-Derived Xenografts in Humanized Mice for Evaluation of Emerging Targeted Therapies and Immunotherapy Combinations for Melanoma. Cancers (Basel) 15:N/A (2023). PubMed: 37509357
- Klersy A et al. Ectodomain Shedding by ADAM17 Increases the Release of Soluble CD40 from Human Endothelial Cells under Pro-Inflammatory Conditions. Cells 12:N/A (2023). PubMed: 37566005
- Yan C et al. Proximity of immune and tumor cells underlies response to BRAF/MEK-targeted therapies in metastatic melanoma patients. NPJ Precis Oncol 6:6 (2022). PubMed: 35058553
- Rogala B et al. Investigation of the Molecular Profile of Granular Cell Tumours and Schwannomas of the Oral Cavity. Dent J (Basel) 10:N/A (2022). PubMed: 35323240