AB108996

重组Anti-CD3G抗体[EPR4516]

Anti-CD3G antibody [EPR4516]

RabMAb
Recombinant
了解详情

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(2 Publications)

Rabbit Recombinant Monoclonal CD3G antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

查看别名

CD3g, T3G, CD3G, T-cell surface glycoprotein CD3 gamma chain, T-cell receptor T3 gamma chain

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3G antibody [EPR4516] (AB108996)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3G antibody [EPR4516] (AB108996)

ab108996 at 1/100 dilution staining CD3G in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-CD3G antibody [EPR4516] (AB108996)

All lanes:

Western blot - Anti-CD3G antibody [EPR4516] (ab108996) at 1/1000 dilution

All lanes:

Jurkat cell lysate at 10 µg

Predicted band size: 20 kDa

false

Unknown

Western blot - Anti-CD3G antibody [EPR4516] (AB108996)

All lanes:

Western blot - Anti-CD3G antibody [EPR4516] (ab108996) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 10 µg

Lane 2:

Human fetal thymus tissue lysate at 10 µg

Lane 3:

HuT-78 cell lysate at 10 µg

Predicted band size: 20 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-CD3G antibody [EPR4516] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4516

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Antigen retrieval is recommended.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface (PubMed : 8187769). Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.

See full target information CD3G

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 26:126 PubMed33297933

2020

The involvement of neuroimmune cells in adipose innervation.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Blaszkiewicz,Elizabeth Wood,Sigi Koizar,Jake Willows,Ryan Anderson,Yu-Hua Tseng,James Godwin,Kristy L Townsend

EBioMedicine 24:216-230 PubMed28888925

2017

Follicular Helper T Cells are Essential for the Elimination of Plasmodium Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Damián Pérez-Mazliah,Minh Phuong Nguyen,Caroline Hosking,Sarah McLaughlin,Matthew D Lewis,Irene Tumwine,Prisca Levy,Jean Langhorne

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-CD3G抗体[EPR4516]