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使用敲除细胞株进行验证重组RabMAb

重组Anti-CD38抗体[EPR4106] - Low endotoxin，Azide free (ab176886)

Reviews (1) Submit a question References (3)
Western blot - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
  • OI-RD Scanning - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
  • Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4106] to CD38 - Low endotoxin, Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human

概述

  • 产品名称

    Anti-CD38抗体[EPR4106] - Low endotoxin，Azide free
    参阅全部 CD38 一抗

  • 描述

    兔单克隆抗体[EPR4106] to CD38 - Low endotoxin，Azide free

  • 宿主

    Rabbit

  • 特异性

    Not suitable for mouse and rat samples because antibody shows a non-specific cytoplasmic staining in almost all tested mouse and rat tissues.

  • 经测试应用

    适用于: IHC-P, WBmore details
    不适用于: Flow Cyt or ICC/IF

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Raji and Ramos cell lysates, Human lymph node lysate. IHC-P: Human endometrial adenocarcinoma, tonsil, lymph node, skeletal muscle and normal colon tissues.

  • 常规说明

    ab176886 is the carrier-free version of ab108403.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab176886于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).
应用说明
Is unsuitable for Flow Cyt or ICC/IF.

靶标

  • 功能

    Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.

  • 组织特异性

    Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.

  • 序列相似性

    Belongs to the ADP-ribosyl cyclase family.

  • 发展阶段

    Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 形式

      There are 2 isoforms produced by alternative splicing.

    • 别名

      • Acute lymphoblastic leukemia cells antigen CD38 antibody
      • ADP ribosyl cyclase 1 antibody
      • ADP ribosyl cyclase antibody
      • ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody
      • ADP-ribosyl cyclase 1 antibody
      • ADPRC 1 antibody
      • ADPRC1 antibody
      • cADPr hydrolase 1 antibody
      • CD 38 antibody
      • CD38 antibody
      • CD38 antigen (p45) antibody
      • CD38 antigen antibody
      • CD38 molecule antibody
      • Cd38-rs1 antibody
      • CD38_HUMAN antibody
      • CD38H antibody
      • Cyclic ADP ribose hydrolase antibody
      • Cyclic ADP ribose hydrolase 1 antibody
      • Cyclic ADP-ribose hydrolase 1 antibody
      • EC 3.2.2.5 antibody
      • Ecto nicotinamide adenine dinucleotide glycohydrolase antibody
      • I-19 antibody
      • I19 (mouse) antibody
      • Lymphocyte differentiation antigen CD38 antibody
      • NAD(+) nucleosidase antibody
      • NIM-R5 antigen antibody
      • NIMR5 antigen (mouse) antibody
      • OTTHUMP00000158633 antibody
      • OTTHUMP00000217743 antibody
      • p45 antibody
      • T10 antibody
      see all

    图片

    • Western blot - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
      Western blot - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
      All lanes : Anti-CD38 antibody [EPR4106] (ab108403) at 1/2000 dilution

      Lane 1 : Wild-type A549 cell lysate
      Lane 2 : CD38 knockout A549 cell lysate
      Lane 3 : Daudi cell lysate
      Lane 4 : HCT 116 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 34 kDa
      Observed band size: 42 kDa why is the actual band size different from the predicted?



      This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab108403).

      Anti-CD38 antibody [EPR4106] staining at 1/2000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108403 was shown to bind specifically to CD38. A band was observed at 42 kDa in wild-type A549 cell lysates with no signal observed at this size in CD38 knockout cell line. To generate this image, wild-type and CD38 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

      This IHC data was generated using the same anti-CD38 antibody clone, EPR4106, in a different buffer formulation (cat# ab108403).

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

      This IHC data was generated using the same anti-CD38 antibody clone, EPR4106, in a different buffer formulation (cat# ab108403).

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial adenocarcinoma tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108403).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108403).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling CD38 with unpurified ab108403.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108403).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human colon tissue labelling CD38 with unpurified ab108403.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108403).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
      OI-RD Scanning - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108403).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

      Tissue Microarrays stained for " Anti-CD38 antibody [EPR4106]” using " ab108403" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with ab108403 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

    • Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
      Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

    文献 (3)

    发表研究结果有使用 ab176886？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab176886 被引用在 3 文献中.

    • Zheng Y  et al. PD-L1+CD8+ T cells enrichment in lung cancer exerted regulatory function and tumor-promoting tolerance. iScience 25:103785 (2022). Mass Cytometry, glucose transporter glut1 . PubMed: 35146396
    • Schwabenland M  et al. Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions. Immunity 54:1594-1610.e11 (2021). PubMed: 34174183
    • Chevrier S  et al. An Immune Atlas of Clear Cell Renal Cell Carcinoma. Cell 169:736-749.e18 (2017). IHC ; Human . PubMed: 28475899

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    Mass Cytometry abreview for Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free

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    Abreviews
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    Application
    Mass Cytometry
    Sample
    Human Cell (liver)
    Specification
    liver
    Read More
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    提交于 Jul 31 2020

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