重组Anti-CD38抗体[EPR4106] - BSA and Azide free (ab226034)
![Western blot - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)](https://www.abcam.cn/ps/products/226/ab226034/Images/ab226034-513166-anti-cd38-antibody-epr4106-western-blot-wildtype-a549-cd38-knockout-a549-daudi-hct-116.jpg "Western blot - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4106] to CD38 - BSA and Azide free
- Suitable for: WB, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD38抗体[EPR4106] - BSA and Azide free
参阅全部 CD38 一抗 -
描述
兔单克隆抗体[EPR4106] to CD38 - BSA and Azide free -
宿主
Rabbit -
特异性
Not suitable for mouse and rat samples because antibody shows a non-specific cytoplasmic staining in almost all tested mouse and rat tissues.
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经测试应用
适用于: WB, IHC-P, Flow Cytmore details
不适用于: ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Raji and Ramos cell lysates, Human lymph node lysate. IHC-P: Human endometrial adenocarcinoma, tonsil, lymph node, skeletal muscle and normal colon tissues. Flow Cyt: Raji cells.
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常规说明
ab226034 is the carrier-free version of ab108403.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解离常数（KD）
KD = 1.40 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4106 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226034于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa). |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. -
组织特异性
Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. -
序列相似性
Belongs to the ADP-ribosyl cyclase family. -
发展阶段
Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 952 Human
- Omim: 107270 Human
- SwissProt: P28907 Human
- Unigene: 479214 Human
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形式
There are 2 isoforms produced by alternative splicing. -
别名
- Acute lymphoblastic leukemia cells antigen CD38 antibody
- ADP ribosyl cyclase 1 antibody
- ADP ribosyl cyclase antibody
see all
图片
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Western blot - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)All lanes : Anti-CD38 antibody [EPR4106] (ab108403) at 1/2000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : CD38 knockout A549 cell lysate
Lane 3 : Daudi cell lysate
Lane 4 : HCT 116 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab108403).
Anti-CD38 antibody [EPR4106] staining at 1/2000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108403 was shown to bind specifically to CD38. A band was observed at 42 kDa in wild-type A549 cell lysates with no signal observed at this size in CD38 knockout cell line. To generate this image, wild-type and CD38 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)](https://www.abcam.cn/ps/products/226/ab226034/Images/ab226034-385404-anticd38antibodyepr4106immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab108403).
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![Flow Cytometry - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)](https://www.abcam.cn/ps/products/226/ab226034/Images/ab226034-385405-anticd38antibodyepr4106flowcytometry-raji-human.jpg)
Flow Cytometry - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)Flow cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labelling CD38 with ab108403 at 1/900 dilution (1.03 µg/ml) (Red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab108403).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)](https://www.abcam.cn/ps/products/226/ab226034/Images/ab226034-385406-anticd38antibodyepr4106immunohistochemistry-skeletal-muscle-negative-control-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab108403).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)](https://www.abcam.cn/ps/products/226/ab226034/Images/ab226034-386709-anti-cd38-antibody-epr4106-ihc-human-tonsil-tissue.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab108403).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)](https://www.abcam.cn/ps/products/226/ab226034/Images/ab226034-386708-anti-cd38-antibody-epr4106-ihc-human-endometrial-adenocarcinoma-tissue.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial adenocarcinoma tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab108403).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)](https://www.abcam.cn/ps/products/226/ab226034/Images/ab226034-386707-anti-cd38-antibody-epr4106-ihc-human-lymph-node-tissue.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling CD38 with unpurified ab108403.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab108403).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)](https://www.abcam.cn/ps/products/226/ab226034/Images/ab226034-386706-anti-cd38-antibody-epr4106-ihc-human-colon-tissue.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human colon tissue labelling CD38 with unpurified ab108403.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab108403).
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![OI-RD Scanning - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)](https://www.abcam.cn/ps/products/226/ab226034/Images/ab226034-386705-anti-cd38-antibody-epr4106-kd.jpg)
OI-RD Scanning - Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab108403).
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![Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)](https://www.abcam.cn/ps/products/226/ab226034/Images/ab226034-10-benefits-of-recombinant-antibodies.png)
Anti-CD38 antibody [EPR4106] - BSA and Azide free (ab226034)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab226034 尚未被引用在任何文献中。
