重组CD36抗体[EPR22509-40](ab252922)|Abcam中文官网

产品名称

Anti-CD36抗体[EPR22509-40]
参阅全部 CD36 一抗
描述

兔单克隆抗体[EPR22509-40] to CD36
宿主

Rabbit
特异性

CD36 expression is low in the normal brain (PMID: 27646002).

Mouse species is recommended based on WB results, we do not guarantee IHC, ICC or FC for mouse. ab252922 doesn't work in mouse and rat liver as it detects no signal at CD36 position and a close nonspecific band.
经测试应用

适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
不适用于: IP
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: U937 whole cell lysate; rat spleen and fat tissue lysates; human lung and placenta tissue lysates, mouse white fat, mouse brown fat, mouse spleen, mouse brain. IHC-P: Human spleen and hepatocellular carcinoma tissue; rat stomach tissue. Flow cyt: THP-1 cells. ICC/IF: U937
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR22509-40
同种型

IgG
研究领域

Alternative Versions
- Anti-CD36 antibody [EPR22509-40] - BSA and Azide free (ab255331)
- Alexa Fluor® 488 Anti-CD36 antibody [EPR22509-40] (ab320068)
Compatible Secondaries
Immunohistochemistry reagents
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise™承诺保证使用ab252922于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB		1/1000. Detects a band of approximately 70-110 kDa (predicted molecular weight: 53 kDa).
IHC-P		1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC is recommend only for human and rat. Unsuitable for mouse.
ICC/IF		1/50. Unsuitable for mouse
Flow Cyt		1/50. Unsuitable for mouse.

说明
WB 1/1000. Detects a band of approximately 70-110 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC is recommend only for human and rat. Unsuitable for mouse.
ICC/IF 1/50. Unsuitable for mouse
Flow Cyt 1/50. Unsuitable for mouse.

应用说明

Is unsuitable for IP.

功能

Multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed:18353783, PubMed:21610069). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed:18753675). Involved in oral fat perception and preferences (PubMed:22240721, PubMed:25822988). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellulare calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed:20037584). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed:16880211).
(Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling.
疾病相关

Platelet glycoprotein IV deficiency
Coronary heart disease 7
序列相似性

Belongs to the CD36 family.
翻译后修饰

N-glycosylated and O-glycosylated with a ratio of 2:1.
Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome (PubMed:21610069, PubMed:18353783). Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids (PubMed:18353783).
细胞定位

Cell membrane. Membrane raft. Golgi apparatus. Apical cell membrane. Upon ligand-binding, internalized through dynamin-dependent endocytosis.
Target information above from: UniProt accession P16671 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 948 Human
- Entrez Gene: 12491 Mouse
- Entrez Gene: 29184 Rat
- Omim: 173510 Human
- SwissProt: P16671 Human
- SwissProt: Q08857 Mouse
- SwissProt: Q07969 Rat
- Unigene: 120949 Human
- Unigene: 18628 Mouse
- Unigene: 406799 Mouse
- Unigene: 102418 Rat
- Unigene: 203220 Rat
see all
别名
- Adipocyte membrane protein antibody
- BDPLT10 antibody
- CD36 antibody
- CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody
- CD36 antigen antibody
- CD36 molecule (thrombospondin receptor) antibody
- CD36 molecule antibody
- CD36_HUMAN antibody
- CHDS7 antibody
- Cluster determinant 36 antibody
- Collagen receptor, platelet antibody
- FAT antibody
- Fatty acid translocase antibody
- Fatty acid transport protein antibody
- Glycoprotein IIIb antibody
- GP IIIb antibody
- GP3B antibody
- GP4 antibody
- GPIIIB antibody
- GPIV antibody
- Leukocyte differentiation antigen CD36 antibody
- MGC108510 antibody
- MGC91634 antibody
- PAS 4 protein antibody
- PAS IV antibody
- PAS-4 antibody
- PASIV antibody
- Platelet collagen receptor antibody
- Platelet glycoprotein 4 antibody
- Platelet glycoprotein IV antibody
- scarb3 antibody
- Scavenger receptor class B member 3 antibody
- SR-B3 antibody
- SRB3 antibody
- Thrombospondin receptor antibody
see all

Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)

All lanes : Anti-CD36 antibody [EPR22509-40] (ab252922) at 1/1000 dilution

Lane 1 : Rat spleen tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Predicted band size: 53 kDa
Observed band size: 70-110 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking buffer: 5% NFDM/TBST

Dilution buffer: 5% NFDM /TBST

CD36 expression is low in the normal brain (PMID:27646002)
Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)

All lanes : Anti-CD36 antibody [EPR22509-40] (ab252922) at 1/1000 dilution

Lane 1 : Mouse white fat tissue lysate
Lane 2 : Mouse brown fat tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Mouse brain tissue lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 53 kDa
Observed band size: 100,70 kDa why is the actual band size different from the predicted?

Exposure time: 81 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The molecular weight observed is due to glycosylation and consistent with what has been described in the literature (PMID:8798390, 15677505, 20637247, 8631821).

Low expression: brain (PMID: 27646002 )

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
Immunocytochemistry/ Immunofluorescence - Anti-CD36 antibody [EPR22509-40] (ab252922)

Immunofluorescent analysis of 80% Methanol fixed 0.1% TritonX-100 permeabilised U937 (Human histiocytic lymphoma monocyte) cells labelling CD36 with ab252922 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution. Confocal image showing membranous staining in U937 cells (Human histiocytic lymphoma monocyte) is observed. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) (ab195889) was used to counterstain Alpha Tubulin at 1/200 dilution.The Nuclear counterstain is DAPI .

Secondary antibody only control: Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (ab150081) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (ab252922)

Immunohistochemical analysis of [paraffin-embedded human spleen tissue labeling CD36 with ab252922 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of human spleen (PMID:30047927,22777768) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (ab252922)

Flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cells labeling CD36 with ab252922 at 1/50 dilution (red) compared with a Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

Goat Anti-Rabbit IgG Fc (Alexa Fluo^r® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)

All lanes : Anti-CD36 antibody [EPR22509-40] (ab252922) at 1000 cells

Lane 1 : Human placenta tissue lysate
Lane 2 : Human lung tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Predicted band size: 53 kDa

Exposure time: 3 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

The molecular weight observed is due to glycosylation and consistent with what has been described in the literature (PMID:8798390,15677505,20637247).
Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)

All lanes : Anti-CD36 antibody [EPR22509-40] (ab252922) at 1/1000 dilution

Lane 1 : U937 (human histiocytic lymphoma cell line) whole cell lysate
Lane 2 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : Rat spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 53 kDa
Observed band size: 70-110 kDa why is the actual band size different from the predicted?

Exposure time: 8 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

Negative control: Ramos, Jurkat (PMID: 27956597,10744776)

The molecular weight observed is due to glycosylation and consistent with what has been described in the literature (PMID:8798390,15677505,20637247, 8631821).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (ab252922)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling CD36 with ab252922 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of rat stomach. (PMID:30047927,22777768) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (ab252922)

Immunohistochemical analysis of paraffin-embedded human hepatocellular tissue labeling CD36 with ab252922 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of human hepatocellular carcinoma (PMID:30047927,22777768) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (ab252922)

Flow cytometric analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling CD36 with ab252922 at 1/50 dilution (red) compared with a Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

Negative control: Ramos (PMID: 10744776).
Anti-CD36 antibody [EPR22509-40] (ab252922)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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发表研究结果有使用 ab252922？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab252922 被引用在 12 文献中.

LaFargue CJ et al. Overcoming adaptive resistance to anti-VEGF therapy by targeting CD5L. Nat Commun 14:2407 (2023). PubMed: 37100807
Meng X et al. Lipid metabolism marker CD36 is associated with 18FDG-PET/CT false negative lymph nodes in head and neck squamous cell carcinoma. Front Oncol 13:1156527 (2023). PubMed: 37207149
Zhu M et al. LRP1 as a promising therapeutic target for gastrointestinal tumors: Inhibiting proliferation, invasion and migration of cancer cells. Oncol Lett 26:432 (2023). PubMed: 37664649
Wang Y et al. Deciphering intratumoral heterogeneity of hepatocellular carcinoma with microvascular invasion with radiogenomic analysis. J Transl Med 21:734 (2023). PubMed: 37853415
Hu M & Liu L Choline regulation of triglycerides synthesis through ubiquintination pathway in MAC-T cells. PeerJ 11:e16611 (2023). PubMed: 38144203

View all Publications for this product

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概述

产品名称

描述

宿主

特异性

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Immunohistochemistry reagents

Isotype control

应用

The Abpromise guarantee

靶标

功能

疾病相关

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

文献 (12)

客户评价及客户问答