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AB126737

重组Anti-CD35抗体[EPR6602]

Anti-CD35 antibody [EPR6602]

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(4 Publications)

Rabbit Recombinant Monoclonal CD35 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

查看别名

CD35, C3BR, CR1, Complement receptor type 1, C3b/C4b receptor

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (AB126737)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (AB126737)

ab126737 at 1/250 dilution staining CD35 in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (AB126737)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (AB126737)

ab126737 at 1/250 dilution staining CD35 in Formalin fixed Paraffin-embedded Human tonsil tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-CD35 antibody [EPR6602] (AB126737)
  • WB

Unknown

Western blot - Anti-CD35 antibody [EPR6602] (AB126737)

All lanes:

Western blot - Anti-CD35 antibody [EPR6602] (ab126737) at 1/1000 dilution

Lane 1:

TF1 lysate at 10 µg

Lane 2:

Human tonsil lysate at 10 µg

Secondary

All lanes:

Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 223 kDa

true

不同偶联物与剂型 (1)

  • Carrier free

    Anti-CD35 antibody [EPR6602] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR6602

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.31% Sodium citrate, 0.175% Sodium chloride, 0.05% BSA, 0.0172% EDTA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Stable for 12 months at -20°C
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Membrane immune adherence receptor that plays a critical role in the capture and clearance of complement-opsonized pathogens by erythrocytes and monocytes/macrophages (PubMed : 2963069). Mediates the binding by these cells of particles and immune complexes that have activated complement to eliminate them from the circulation (PubMed : 2963069). Acts also in the inhibition of spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d,g, and of C4b to C4c and C4d (PubMed : 2972794, PubMed : 8175757). Also plays a role in immune regulation by contributing, upon ligand binding, to the generation of regulatory T cells from activated helper T cells (PubMed : 25742728).. (Microbial infection) Acts as a receptor for Epstein-Barr virus.
See full target information CR1
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 220: PubMed37036425

2023

Functional specialization of short-lived and long-lived macrophage subsets in human tonsils.

Applications

Unspecified application

Species

Unspecified reactive species

Lamine Alaoui,Javiera Villar,Renaud Leclere,Simon Le Gallou,Francis Relouzat,Henri-Alexandre Michaud,Karin Tarte,Natacha Teissier,Benoît Favier,Mikaël Roussel,Elodie Segura

BMC genetics 21:101 PubMed32907542

2020

A novel mouse model expressing human forms for complement receptors CR1 and CR2.

Applications

Unspecified application

Species

Unspecified reactive species

Harriet M Jackson,Kate E Foley,Rita O'Rourke,Timothy M Stearns,Dina Fathalla,B Paul Morgan,Gareth R Howell

Diabetologia 60:1294-1303 PubMed28213757

2017

Germinal centre frequency is decreased in pancreatic lymph nodes from individuals with recent-onset type 1 diabetes.

Applications

Unspecified application

Species

Human

Abby Willcox,Sarah J Richardson,Lucy S K Walker,Sally C Kent,Noel G Morgan,Kathleen M Gillespie

Leukemia & lymphoma 57:1687-96 PubMed26700650

2015

Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues.

Applications

IHC

Species

Human

Rintaro Ohe,Naing Ye Aung,Hongxue Meng,Takanobu Kabasawa,Aya Suto,Nobuyuki Tamazawa,Suran Yang,Tomoya Kato,Mitsunori Yamakawa
View all publications
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