重组Anti-CD35抗体[EPR6601]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6601] (AB133293)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD35 with purified ab133293 at 1 : 250 dilution (1.76 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6601] (AB133293)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD35 with 1/100 unpurified ab133293.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6601] (AB133293)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling CD35 with unpurified ab133293 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-CD35 antibody [EPR6601] (AB133293)

All lanes:

Western blot - Anti-CD35 antibody [EPR6601] (ab133293) at 1/10000 dilution

All lanes:

Human tonsil lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 223 kDa

Observed band size: 280 kDa

false

• WB

Lab

Western blot - Anti-CD35 antibody [EPR6601] (AB133293)

All lanes:

Western blot - Anti-CD35 antibody [EPR6601] (ab133293) at 1/1000 dilution

All lanes:

TF-1 (Human Erythroleukemia erythroblast) whole cell lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 223 kDa

Observed band size: 280 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CD35 antibody [EPR6601] (AB133293)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.