Anti-CD35抗体[E11] - BSA and Azide free (ab25)
Key features and details
- Mouse monoclonal [E11] to CD35 - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-CD35抗体[E11] - BSA and Azide free
参阅全部 CD35 一抗 -
描述
小鼠单克隆抗体[E11] to CD35 - BSA and Azide free -
宿主
Mouse -
特异性
Does not block complement receptor 1 (CR1) activity. No cross reaction with CR2. -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD35. human acute monocytic leukaemia cells.
Database link: P17927 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E11 -
骨髓瘤
P3-NS1/1-Ag4-1 -
同种型
IgG -
轻链类型
unknown -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
应用
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Mediates cellular binding of particles and immune complexes that have activated complement. -
组织特异性
Present on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. -
序列相似性
Belongs to the receptors of complement activation (RCA) family.
Contains 30 Sushi (CCP/SCR) domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1378 Human
- Omim: 120620 Human
- SwissProt: P17927 Human
- Unigene: 334019 Human
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别名
- C3 binding protein antibody
- C3b/C4b receptor antibody
- C3BR antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 [E11] antibody - BSA and Azide free (ab25)ab25 (1µg/ml) staining CD35 in human spleen.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab25 被引用在 11 文献中.
- Blanch AJ et al. Multimodal imaging reveals membrane skeleton reorganisation during reticulocyte maturation and differences in dimple and rim regions of mature erythrocytes. J Struct Biol X 6:100056 (2022). PubMed: 34977554
- Yuan K et al. Complement C3 overexpression activates JAK2/STAT3 pathway and correlates with gastric cancer progression. J Exp Clin Cancer Res 39:9 (2020). PubMed: 31928530
- Kaneko N et al. Loss of Bcl-6-Expressing T Follicular Helper Cells and Germinal Centers in COVID-19. Cell 183:143-157.e13 (2020). PubMed: 32877699
- Agarwal M et al. Reactive oxygen species-mediated cytoplasmic stiffening impairs the phagocytic ability of the macrophage. J Cell Sci 133:N/A (2020). PubMed: 32005700
- Su R et al. NGF protects neuroblastoma cells against ß-amyloid-induced apoptosis via the Nrf2/HO-1 pathway. FEBS Open Bio 9:2063-2071 (2019). PubMed: 31605506