重组Anti-CD34抗体[RM2052] - BSA and Azide free (ab317589)
![Western blot - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)](https://www.abcam.cn/ps/products/317/ab317589/Images/ab317589-540592-anti-cd34-antibody-rm2052-western-blot-bend3-mouse-brain-endothelial-cell-whole-cell-lysate-20-ug.jpg "Western blot - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [RM2052] to CD34 - BSA and Azide free
- Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CD34抗体[RM2052] - BSA and Azide free
参阅全部 CD34 一抗 -
描述
兔重组multiclonal [RM2052] to CD34 - BSA and Azide free -
宿主
Rabbit -
特异性
Unsuitable for mouse ICC.
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经测试应用
适用于: IHC-P, ICC/IF, Flow Cyt, WBmore details
不适用于: IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers. -
阳性对照
- WB: bEnd.3 whole cell, NIH/3T3 whole cell, KG-1a whole cell, Mouse testis tissue, Mouse lung tissue lysates. IHC-P: Human placenta, Human kidney, Human colon cancer, Mouse kidney, Mouse lung, Rat kidney and Rat lung tissues. ICC/IF: KG-1a and Daudi cells. Flow Cyt: NIH/3T3, KG-1a and Mouse bone marrow cells.
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常规说明
ab317589 is the carrier-free version of ab317588
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
Recombinant Multiclonal -
克隆编号
RM2052 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317589于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| ICC/IF |
Use at an assay dependent concentration.
|
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| Flow Cyt |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. |
靶标
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功能
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
组织特异性
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
序列相似性
Belongs to the CD34 family. -
发展阶段
On early hematopoietic progenitor cells. -
翻译后修饰
Highly glycosylated.
Phosphorylated on serine residues by PKC. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 947 Human
- Entrez Gene: 12490 Mouse
- Entrez Gene: 305081 Rat
- Omim: 142230 Human
- SwissProt: P28906 Human
- SwissProt: Q64314 Mouse
- Unigene: 374990 Human
- Unigene: 29798 Mouse
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别名
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
图片
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Western blot - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)All lanes : Anti-CD34 antibody [RM2052] (ab317588) at 1/1000 dilution
Lane 1 : bEnd.3 (mouse brain endothelial cell) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 80-120 kDa why is the actual band size different from the predicted?This data was developed using ab317588, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: Neuro-2a.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 37181754, 29296932).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1: 15 seconds, lanes 2-3: 180 seconds
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![Western blot - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)](https://www.abcam.cn/ps/products/317/ab317589/Images/ab317589-540593-anti-cd34-antibody-rm2052-western-blot-kg-1a-human-bone-marrow-myeloblast-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)All lanes : Anti-CD34 antibody [RM2052] (ab317588) at 1/1000 dilution
Lane 1 : KG-1a (human bone marrow myeloblast) whole cell lysate
Lane 2 : Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate
Lane 3 : HuT-78 (human Sezary syndrome cutaneous T lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 80-120 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsThis data was developed using ab317588, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: Daudi (PMID: 21286385), HuT-78.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 37181754, 29296932).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Western blot - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)](https://www.abcam.cn/ps/products/317/ab317589/Images/ab317589-540594-anti-cd34-antibody-rm2052-western-blot-mouse-testis-tissue-lysate-20-ug.jpg)
Western blot - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)All lanes : Anti-CD34 antibody [RM2052] (ab317588) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Mouse liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 80-120 kDa why is the actual band size different from the predicted?This data was developed using ab317588, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: liver (PMID:1709048).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 37181754, 29296932).
The identity of the lower MW band at approximately 40 kDa (in lane 3) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Exposure time: Lane 2: 10 seconds, lanes 1 and 3: 180 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)](https://www.abcam.cn/ps/products/317/ab317589/Images/ab317589-1-anti-cd34-antibody-rm2052-immunohistochemistry-human-placenta.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)This data was developed using ab317588, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CD34 with ab317588 at 1/2000 (0.247 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on endothelium of human placenta (PMID: 35263417). The section was incubated with ab317588 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)](https://www.abcam.cn/ps/products/317/ab317589/Images/ab317589-2-anti-cd34-antibody-rm2052-immunohistochemistry-human-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)This data was developed using ab317588, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CD34 with ab317588 at 1/2000 (0.247 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on endothelium of human kidney (PMID: 16234507). The section was incubated with ab317588 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)](https://www.abcam.cn/ps/products/317/ab317589/Images/ab317589-3-anti-cd34-antibody-rm2052-immunohistochemistry-human-colon-cancer.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)This data was developed using ab317588, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling CD34 with ab317588 at 1/2000 (0.247 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on endothelium of human colon cancer. The section was incubated with ab317588 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)](https://www.abcam.cn/ps/products/317/ab317589/Images/ab317589-4-anti-cd34-antibody-rm2052-immunohistochemistry-mouse-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)This data was developed using ab317588, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling CD34 with ab317588 at 1/5000 (0.099 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on endothelium of mouse kidney. The section was incubated with ab317588 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)](https://www.abcam.cn/ps/products/317/ab317589/Images/ab317589-5-anti-cd34-antibody-rm2052-immunohistochemistry-mouse-lung.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)This data was developed using ab317588, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling CD34 with ab317588 at 1/5000 (0.099 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on endothelium of mouse lung. The section was incubated with ab317588 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)](https://www.abcam.cn/ps/products/317/ab317589/Images/ab317589-6-anti-cd34-antibody-rm2052-immunohistochemistry-rat-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)This data was developed using ab317588, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling CD34 with ab317588 at 1/5000 (0.099 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on endothelium of rat kidney. The section was incubated with ab317588 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)](https://www.abcam.cn/ps/products/317/ab317589/Images/ab317589-7-anti-cd34-antibody-rm2052-immunohistochemistry-rat-lung.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)This data was developed using ab317588, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling CD34 with ab317588 at 1/5000 (0.099 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on endothelium of rat lung. The section was incubated with ab317588 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
-
![Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)](https://www.abcam.cn/ps/products/317/ab317589/Images/ab317589-8-anti-cd34-antibody-rm2052-immunocytochemistry-kg-1a.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)This data was developed using ab317588, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized KG-1a (human bone marrow myeloblast) cells labelling CD34 with ab317588 at 1/50 (9.88 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing positive staining in KG-1a cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Low expression: Daudi (PMID: 21286385).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
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![Flow Cytometry - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)](https://www.abcam.cn/ps/products/317/ab317589/Images/ab317589-9-anti-cd34-antibody-rm2052-flow-cytometry-neuro-2a.jpg)
Flow Cytometry - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)This data was developed using ab317588, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Neuro-2a(mouse neuroblastoma neuroblast, Left) / NIH/3T3(mouse embryonic fibroblast, Right) cells labelling CD34 with ab317588 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cell.
Low expression: Neuro-2a. -
![Flow Cytometry - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)](https://www.abcam.cn/ps/products/317/ab317589/Images/ab317589-10-anti-cd34-antibody-rm2052-flow-cytometry-daudi.jpg)
Flow Cytometry - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)This data was developed using ab317588, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Daudi(human Burkitt's lymphoma lymphoblast, Left) / KG-1a(human bone marrow myeloblast, Right) cells labelling CD34 with ab317588 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cell.
Low expression: Daudi. -
![Flow Cytometry - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)](https://www.abcam.cn/ps/products/317/ab317589/Images/ab317589-11-anti-cd34-antibody-rm2052-flow-cytometry-mouse-bone-marrow.jpg)
Flow Cytometry - Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)This data was developed using ab317588, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Mouse bone marrow cells labelling CD34 with ab317588 at 1/500 dilution (0.1ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Mouse bone marrow are co-stained with CD117 conjugated APC-eFluor 780.
Gated on viable cell. -
![Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)](https://www.abcam.cn/ps/products/317/ab317589/Images/ab317589-12-benefitsofrecombinantantibodies.jpg)
Anti-CD34 antibody [RM2052] - BSA and Azide free (ab317589)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab317589 尚未被引用在任何文献中。
