重组Anti-CD33抗体[WM53] - BSA and Azide free (ab252263)
![Immunocytochemistry/ Immunofluorescence - Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)](https://www.abcam.cn/ps/products/252/ab252263/Images/ab252263-381328-anticd33wm53icchl60.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [WM53] to CD33 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD33抗体[WM53] - BSA and Azide free
参阅全部 CD33 一抗 -
描述
小鼠单克隆抗体[WM53] to CD33 - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: ICC/IF, Flow Cytmore details
不适用于: WB -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt: Human peripheral blood mononuclear cells (PBMC). ICC: HL-60 cells
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常规说明
ab252263 is the carrier-free version of ab30371.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
WM53 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
- APC Anti-CD33 antibody [WM53] (ab118967)
- PerCP Anti-CD33 antibody [WM53] (ab124531)
- APC/Cy7® Anti-CD33 antibody [WM53] (ab206634)
- PE/Cy5® Anti-CD33 antibody [WM53] (ab233448)
- PE Anti-CD33 antibody [WM53] (ab233577)
- FITC Anti-CD33 antibody [WM53] (ab234216)
- PE/Cy7® Anti-CD33 antibody [WM53] (ab239310)
- Anti-CD33 antibody [WM53] (ab30371)
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab252263于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
Works in both 4% PFA fixed cells (10mins) and 100% MeOH fixed cells (5mins) |
|
| Flow Cyt |
1/500.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. Works in both 4% PFA fixed cells (10mins) and 100% MeOH fixed cells (5mins) |
|
Flow Cyt
1/500. |
靶标
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功能
Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). -
组织特异性
Monocytic/myeloid lineage cells. -
序列相似性
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
结构域
Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
翻译后修饰
Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 945 Human
- Omim: 159590 Human
- SwissProt: P20138 Human
- Unigene: 83731 Human
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别名
- CD 33 antibody
- CD33 antibody
- CD33 antigen (gp67) antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab30371)
ab30371 staining CD33 in HL-60 cells. The cells were fixed with 100% MeOH (5min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab30371 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Flow Cytometry - Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)](https://www.abcam.cn/ps/products/252/ab252263/Images/ab252263-368861-AntiCD33-antibody-WM53-flow-cytometry-human-peripheral-blood-mononuclear-cells.jpg)
Flow Cytometry - Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) (right) labeling CD33 with ab30371 at 1/40 dilution compared with mouse monoclonal IgG Isotype Control (left). Goat anti-mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with mouse IgG (Left) or ab30371 (Right). Then stained with anti-CD14 conjugated to Alexa Fluor® 647.
Gated on viable cells.
This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab30371).
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![Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)](https://www.abcam.cn/ps/products/252/ab252263/Images/ab252263-4-benefits-of-recombinant-antibodies.png)
Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (8)
ab252263 被引用在 8 文献中.
- Ornatsky O et al. Highly multiparametric analysis by mass cytometry. J Immunol Methods 361:1-20 (2010). PubMed: 20655312
- Hernández-Caselles T et al. A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing. J Leukoc Biol 79:46-58 (2006). PubMed: 16380601
- Garnache-Ottou F et al. Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells. Blood 105:1256-64 (2005). PubMed: 15388576
- Schenk M et al. Macrophages expressing triggering receptor expressed on myeloid cells-1 are underrepresented in the human intestine. J Immunol 174:517-24 (2005). PubMed: 15611278
- Leone AM et al. Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function. Eur Heart J 26:1196-204 (2005). PubMed: 15734770
- Hernández-López C et al. Stromal cell-derived factor 1/CXCR4 signaling is critical for early human T-cell development. Blood 99:546-54 (2002). PubMed: 11781237
- Shin YK et al. Expression of leukemia-associated antigen, JL1, in bone marrow and thymus. Am J Pathol 158:1473-80 (2001). PubMed: 11290565
- Vitale C et al. Surface expression and function of p75/AIRM-1 or CD33 in acute myeloid leukemias: engagement of CD33 induces apoptosis of leukemic cells. Proc Natl Acad Sci U S A 98:5764-9 (2001). PubMed: 11320212
