Anti-CD32B (phospho Y292)抗体(ab74092)
Key features and details
- Rabbit polyclonal to CD32B (phospho Y292)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CD32B (phospho Y292)抗体
参阅全部 CD32B 一抗 -
描述
兔多克隆抗体to CD32B (phospho Y292) -
宿主
Rabbit -
特异性
ab74092 detects endogenous levels of CD32 only when phosphorylated at tyrosine 292. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human CD32B (phospho Y292).
Database link: P31994 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab74092 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74092于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
靶标
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功能
Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis. -
组织特异性
Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells. -
疾病相关
Note=A chromosomal aberration involving FCGR2B is found in a follicular lymphoma. Translocation t(1;22)(q22;q11). The translocation leads to the hyperexpression of the receptor. This may play a role in the tumor progression. -
序列相似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
结构域
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2213 Human
- Omim: 604590 Human
- SwissProt: P31994 Human
- Unigene: 654395 Human
- Unigene: 713617 Human
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别名
- CD32 antibody
- CD32 antigen antibody
- CD32B antibody
see all
图片
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Western blot - Anti-CD32B (phospho Y292) antibody (ab74092)All lanes : Anti-CD32B (phospho Y292) antibody (ab74092) at 1/500 dilution
Lane 1 : K562 cell extracts treated
with PMA (125ng/ml, 30mins)
Lane 2 : K562 cell extracts treated
with PMA (125ng/ml, 30mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDa
Observed band size: 34 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab74092 尚未被引用在任何文献中。