Anti-CD32B + CD32A抗体[AT10] - BSA free (ab41899)
Key features and details
- Mouse monoclonal [AT10] to CD32B + CD32A - BSA free
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-CD32B + CD32A抗体[AT10] - BSA free -
描述
小鼠单克隆抗体[AT10] to CD32B + CD32A - BSA free -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Dog -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
无载体
是 -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
AT10 -
骨髓瘤
NS1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab41899于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt (Intra)
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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细胞定位
CD32B: Cell membrane. CD32A: Cell membrane. -
数据库链接
- Entrez Gene: 2212 Human
- Entrez Gene: 2213 Human
- Omim: 146790 Human
- Omim: 604590 Human
- SwissProt: P12318 Human
- SwissProt: P31994 Human
- Unigene: 352642 Human
- Unigene: 654395 Human
see all
图片
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Overlay histogram showing K562 cells stained with ab41899 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41899, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed K562 cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab41899 被引用在 14 文献中.
- Valdoz JC et al. Soluble ECM promotes organotypic formation in lung alveolar model. Biomaterials 283:121464 (2022). PubMed: 35306229
- Zhang L et al. C-reactive protein exacerbates epithelial-mesenchymal transition through Wnt/ß-catenin and ERK signaling in streptozocin-induced diabetic nephropathy. FASEB J 33:6551-6563 (2019). PubMed: 30794428
- Karagiannis P et al. IgG4 subclass antibodies impair antitumor immunity in melanoma. J Clin Invest 123:1457-74 (2013). IHC-Fr ; Human . PubMed: 23454746
- Municio C et al. The response of human macrophages to ß-glucans depends on the inflammatory milieu. PLoS One 8:e62016 (2013). IP ; Human . PubMed: 23637950
- Morello A et al. Enhancing production and cytotoxic activity of polymeric soluble FasL-based chimeric proteins by concomitant expression of soluble FasL. PLoS One 8:e73375 (2013). PubMed: 23991192