Anti-CD32B + CD32A抗体[AT10] (ab23336)
![Immunocytochemistry/ Immunofluorescence - Anti-CD32B + CD32A antibody [AT10] (ab23336)](https://www.abcam.cn/ps/products/23/ab23336/Images/ab23336-5-anti-cd32-cd32a-at10-antibody-immunocytochemistry-human-k-562.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD32B + CD32A antibody [AT10] (ab23336)")
Key features and details
- Mouse monoclonal [AT10] to CD32B + CD32A
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-CD32B + CD32A抗体[AT10] -
描述
小鼠单克隆抗体[AT10] to CD32B + CD32A -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra), ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Dog, Rhesus monkey
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免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (Intra): K562 cells. ICC/IF: K562 cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
AT10 -
骨髓瘤
NS1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23336于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Flow Cyt (Intra)
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
Use at an assay dependent concentration. |
靶标
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细胞定位
CD32B: Cell membrane. CD32A: Cell membrane. -
数据库链接
- Entrez Gene: 2212 Human
- Entrez Gene: 2213 Human
- Omim: 146790 Human
- Omim: 604590 Human
- SwissProt: P12318 Human
- SwissProt: P31994 Human
- Unigene: 352642 Human
- Unigene: 654395 Human
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-CD32B + CD32A antibody [AT10] (ab23336)
ab23336 staining CD32B + CD32A in K-562 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab23336 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown..
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![Flow Cytometry (Intracellular) - Anti-CD32B + CD32A antibody [AT10] (ab23336)](https://www.abcam.cn/ps/products/23/ab23336/Images/ab23336-32852-anti-cd32-antibody-at10-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-CD32B + CD32A antibody [AT10] (ab23336)Overlay histogram showing K562 cells stained with ab23336 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23336, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed K562 cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab23336 被引用在 12 文献中.
- Bogie JFJ et al. Stearoyl-CoA desaturase-1 impairs the reparative properties of macrophages and microglia in the brain. J Exp Med 217:N/A (2020). PubMed: 32097464
- Lande R et al. CXCL4 assembles DNA into liquid crystalline complexes to amplify TLR9-mediated interferon-a production in systemic sclerosis. Nat Commun 10:1731 (2019). PubMed: 31043596
- de Stoppelaar SF et al. Toll-Like Receptor Signalling Is Not Involved in Platelet Response to Streptococcus pneumoniae In Vitro or In Vivo. PLoS One 11:e0156977 (2016). PubMed: 27253707
- Bakema JE et al. Antibody-opsonized bacteria evoke an inflammatory dendritic cell phenotype and polyfunctional Th cells by cross-talk between TLRs and FcRs. J Immunol 194:1856-66 (2015). PubMed: 25582855
- Cox N et al. Distinct Fc? receptors mediate the effect of serum amyloid p on neutrophil adhesion and fibrocyte differentiation. J Immunol 193:1701-8 (2014). PubMed: 25024390