Anti-CD32A抗体[OTI9C6] (ab124408)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)](https://www.abcam.cn/ps/products/124/ab124408/Images/ab124408-138112-anti-cd32-antibody-oti9c6-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)")
Key features and details
- Mouse monoclonal [OTI9C6] to CD32A
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human, African green monkey
- Isotype: IgG1
概述
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产品名称
Anti-CD32A抗体[OTI9C6]
参阅全部 CD32A 一抗 -
描述
小鼠单克隆抗体[OTI9C6] to CD32A -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IF, IPmore details -
种属反应性
与反应: Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human CD32A.
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阳性对照
- HEK293T cell lysate transfected with pCMV6-ENTRY CD32; Human Breast, Colon, Kidney, Lung, Ovary, Ovary adenocarcinoma, Pancreas, Thyroid, Thyroid carcinoma, Endometrium and Prostate tissues; COS7 cells transiently transfected with pCMV6-ENTRY CD32;
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常规说明
Dilute in PBS (pH7.3) before use. Avoid repeated freeze-thaws.
The clone number has been updated from 9C6 to OTI9C6, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified from TCS -
克隆
单克隆 -
克隆编号
OTI9C6 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124408于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 35 kDa.
|
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| IHC-P |
1/50.
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| ICC/IF |
1/100.
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| IP |
Use a concentration of 4 µg/ml.
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| 说明 |
|---|
|
WB
1/2000. Predicted molecular weight: 35 kDa. |
|
IHC-P
1/50. |
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ICC/IF
1/100. |
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IP
Use a concentration of 4 µg/ml. |
靶标
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功能
Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. -
组织特异性
Found on monocytes, neutrophils and eosinophil platelets. -
序列相似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
翻译后修饰
Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2212 Human
- Omim: 146790 Human
- SwissProt: P12318 Human
- Unigene: 352642 Human
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别名
- CD32 antibody
- CD32 antigen antibody
- CDw32 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human breast tissue by Immunohistochemistry.
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![Western blot - Anti-CD32A antibody [OTI9C6] (ab124408)](https://www.abcam.cn/ps/products/124/ab124408/Images/ab124408-138114-anti-cd32-antibody-oti9c6-western-blot.jpg)
Western blot - Anti-CD32A antibody [OTI9C6] (ab124408)All lanes : Anti-CD32A antibody [OTI9C6] (ab124408) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CD32
Lysates/proteins at 5 µg per lane.
Predicted band size: 35 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC205503) -
![Immunoprecipitation - Anti-CD32A antibody [OTI9C6] (ab124408)](https://www.abcam.cn/ps/products/124/ab124408/Images/ab124408-138113-anti-cd32-antibody-oti9c6-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD32A antibody [OTI9C6] (ab124408)Immunoprecipitation of CD32A. Lane 1: Negative control, no IgG. Lane2: 2µg of ab124408 and 20µl (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed with lysate and incubated overnight. For each experiment, 500µl of DDK tagged CD32 overexpression lysates (at 1:5 dilution with HEK293T lysate) was used. Predicted band size : 35 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)](https://www.abcam.cn/ps/products/124/ab124408/Images/ab124408-138111-anti-cd32-antibody-oti9c6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human colon tissue by Immunohistochemistry.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)](https://www.abcam.cn/ps/products/124/ab124408/Images/ab124408-138110-anti-cd32-antibody-oti9c6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human kidney tissue by Immunohistochemistry.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)](https://www.abcam.cn/ps/products/124/ab124408/Images/ab124408-138109-anti-cd32-antibody-oti9c6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human lung tissue by Immunohistochemistry.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)](https://www.abcam.cn/ps/products/124/ab124408/Images/ab124408-138108-anti-cd32-antibody-oti9c6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human ovary tissue by Immunohistochemistry.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)](https://www.abcam.cn/ps/products/124/ab124408/Images/ab124408-138107-anti-cd32-antibody-oti9c6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)](https://www.abcam.cn/ps/products/124/ab124408/Images/ab124408-138106-anti-cd32-antibody-oti9c6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)](https://www.abcam.cn/ps/products/124/ab124408/Images/ab124408-138105-anti-cd32-antibody-oti9c6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human thyroid tissue by Immunohistochemistry.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)](https://www.abcam.cn/ps/products/124/ab124408/Images/ab124408-138104-anti-cd32-antibody-oti9c6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)](https://www.abcam.cn/ps/products/124/ab124408/Images/ab124408-138103-anti-cd32-antibody-oti9c6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)](https://www.abcam.cn/ps/products/124/ab124408/Images/ab124408-138102-anti-cd32-antibody-oti9c6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human prostate tissue by Immunohistochemistry.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD32A antibody [OTI9C6] (ab124408)](https://www.abcam.cn/ps/products/124/ab124408/Images/ab124408-138101-anti-cd32-antibody-oti9c6-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD32A antibody [OTI9C6] (ab124408)ab124408, at 1/100 dilution, staining CD32A in COS7 cells transiently transfected with pCMV6-ENTRY CD32 by Immunofluorescence.
实验方案
数据表及文件
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SDS download
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Datasheet download
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