重组Anti-CD31抗体[MEC 7.46] (ab7388)
![Flow Cytometry - Anti-CD31 antibody [MEC 7.46] (ab7388)](https://www.abcam.cn/ps/products/7/ab7388/Images/ab7388-459023-anti-cd31-antibody-flow-cytometry.jpg "Flow Cytometry - Anti-CD31 antibody [MEC 7.46] (ab7388)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [MEC 7.46] to CD31
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-CD31抗体[MEC 7.46]
参阅全部 CD31 一抗 -
描述
大鼠单克隆抗体[MEC 7.46] to CD31 -
宿主
Rat -
特异性
The antibody reacts with the murine form of the Platelet-Endothelial Cell Adhesion Molecule. The reactivity of the antibody is restricted to the isoform of the molecule that is selectively expressed by endothelial cells. The antibody precipitates a 130 kDa molecule present on the membrane of endothelial cell presents on all mouse blood vessels both in normal and inflamed or tumor tissues. The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1. -
经测试应用
适用于: Flow Cyt, ICC/IFmore details
不适用于: IP or WB -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC: mouse bEnd.3 cell line; Flow Cyt: mouse bEnd.3 cell line
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常规说明
This product has switched from a hybridoma to recombinant production method on 08th March 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.5% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
MEC 7.46 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
1/1000.
|
|
| ICC/IF | (1) |
1/50.
|
| 说明 |
|---|
|
Flow Cyt
1/1000. |
|
ICC/IF
1/50. |
靶标
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功能
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC). -
组织特异性
Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level). -
序列相似性
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
结构域
The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity. -
翻译后修饰
Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. -
细胞定位
Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. - Information by UniProt
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数据库链接
- Entrez Gene: 18613 Mouse
- SwissProt: Q08481 Mouse
- Unigene: 343951 Mouse
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别名
- Adhesion molecule antibody
- CD31 antibody
- CD31 antigen antibody
see all
图片
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Flow Cytometry - Anti-CD31 antibody [MEC 7.46] (ab7388)
Flow cytometric analysis of / fixed / permeabilized B16-F0 (Mouse melanoma epithelial cell-like, Left) / bEnd.3 (Mouse brain endothelioma, Right) cells labelling CD31 with ab7388 at 1/1000 dilution (0.1µg) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Negative control: B16-F0.Gated on viable cells.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [MEC 7.46] (ab7388)](https://www.abcam.cn/ps/products/7/ab7388/Images/ab7388-459013-anti-cd31-antibody-immunocytochemistry-bend3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [MEC 7.46] (ab7388)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized bEnd.3 cells labelling CD31 with ab7388 at 1/50 (19.32 µg/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in bEnd.3 cell line. Negative control: B16-F0 (PMID: 8082649) ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Anti-CD31 antibody [MEC 7.46] (ab7388)](https://www.abcam.cn/ps/products/7/ab7388/Images/ab7388-459160-ab187831459157ab256126458994benefitsofrecombinantantibodies.jpg)
Anti-CD31 antibody [MEC 7.46] (ab7388)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (134)
ab7388 被引用在 134 文献中.
- Song H et al. Expression dynamics of lymphoid enhancer-binding factor 1 in terminal Schwann cells, dermal papilla, and interfollicular epidermis. Dev Dyn 252:527-535 (2023). PubMed: 36576725
- Abby E et al. Notch1 mutations drive clonal expansion in normal esophageal epithelium but impair tumor growth. Nat Genet 55:232-245 (2023). PubMed: 36658434
- Zhang T et al. The Aldose Reductase Inhibitor Epalrestat Maintains Blood-Brain Barrier Integrity by Enhancing Endothelial Cell Function during Cerebral Ischemia. Mol Neurobiol 60:3741-3757 (2023). PubMed: 36940077
- Endo Y et al. VEGFA Promotes Skeletal Muscle Regeneration in Aging. Adv Biol (Weinh) 7:e2200320 (2023). PubMed: 36988414
- Dong G et al. Interleukin (IL)-1 Receptor Signaling Is Required for Complete Taste Bud Regeneration and the Recovery of Neural Taste Responses following Axotomy. J Neurosci 43:3439-3455 (2023). PubMed: 37015809