重组Anti-CD31抗体[EPR17260-263] (ab222783)
![Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR17260-263] (ab222783)](https://www.abcam.cn/ps/products/222/ab222783/Images/ab222783-477257-anti-cd31-antibody-epr17260-263-immunocytochemistry.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR17260-263] (ab222783)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17260-263] to CD31
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
概述
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产品名称
Anti-CD31抗体[EPR17260-263]
参阅全部 CD31 一抗 -
描述
兔单克隆抗体[EPR17260-263] to CD31 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: bEND.3 whole cell lysate; Mouse platelet and lung lysates; Rat lung lysate. ICC/IF: bEND.3 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17260-263 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab222783于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100.
Use 0.3M glycine to quench autofluorescence caused by aldehydes. Positive Control: HUVEC cells |
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| WB | (2) |
1/2000. Detects a band of approximately 110-130 kDa (predicted molecular weight: 82 kDa).
In WB, under our testing conditions, we observe an additional band (~50 kDa) for some human, mouse and rat cell and tissue lysates. Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Ms Isoform 1-4: 69.8-81.3 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate (ab7179). |
| 说明 |
|---|
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ICC/IF
1/100. Use 0.3M glycine to quench autofluorescence caused by aldehydes. Positive Control: HUVEC cells |
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WB
1/2000. Detects a band of approximately 110-130 kDa (predicted molecular weight: 82 kDa). In WB, under our testing conditions, we observe an additional band (~50 kDa) for some human, mouse and rat cell and tissue lysates. Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Ms Isoform 1-4: 69.8-81.3 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate (ab7179). |
靶标
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功能
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC). -
组织特异性
Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level). -
序列相似性
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
结构域
The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity. -
翻译后修饰
Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. -
细胞定位
Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. - Information by UniProt
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数据库链接
- Entrez Gene: 18613 Mouse
- Entrez Gene: 29583 Rat
- SwissProt: Q08481 Mouse
- SwissProt: Q3SWT0 Rat
- Unigene: 343951 Mouse
- Unigene: 1878 Rat
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别名
- Adhesion molecule antibody
- CD31 antibody
- CD31 antigen antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR17260-263] (ab222783)
Immunofluorescent analysis of 4% paraformaldehyde-fixed. 0.1% Triton X-100 permeabilized bEND.3 (mouse brain endothelioma cell line) and NIH/3T3 (mouse embyro fibroblast cell line) cells labeling CD31 with ab222783 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on bEND.3 cells. Negative control: NIH/3T3 (PMID: 1429859).
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783)](https://www.abcam.cn/ps/products/222/ab222783/Images/ab222783-281562-anti-cd31-antibody-epr17260-263-western-blot.jpg)
Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783)All lanes : Anti-CD31 antibody [EPR17260-263] (ab222783) at 1/2000 dilution
Lane 1 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 82 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Negative control: NIH/3T3 (PMID: 1429859).
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![Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783)](https://www.abcam.cn/ps/products/222/ab222783/Images/ab222783-281563-anti-cd31-antibody-epr17260-263-western-blot.jpg)
Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783)All lanes : Anti-CD31 antibody [EPR17260-263] (ab222783) at 1/2000 dilution
Lane 1 : Mouse platelet lysate
Lane 2 : Rat lung lysate
Lane 3 : Mouse lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 82 kDa
Observed band size: 110-130 kDa why is the actual band size different from the predicted?Exposure time : Lane 1-2: 3 minutes; Lane 3: 5 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 10448867).
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![Anti-CD31 antibody [EPR17260-263] (ab222783)](https://www.abcam.cn/ps/products/222/ab222783/Images/ab222783-4-benefits-of-recombinant-antibodies.png)
Anti-CD31 antibody [EPR17260-263] (ab222783)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (72)
ab222783 被引用在 72 文献中.
- Zhang PH et al. Efficient pulmonary lymphatic drainage is necessary for inflammation resolution in ARDS. JCI Insight 9:N/A (2024). PubMed: 37971881
- Yang YL et al. YAP1/Piezo1 involve in the dynamic changes of lymphatic vessels in UVR-induced photoaging progress to squamous cell carcinoma. J Transl Med 21:820 (2023). PubMed: 37974224
- Feng X et al. CCL6 promotes M2 polarization and inhibits macrophage autophagy by activating PI3-kinase/Akt signalling pathway during skin wound healing. Exp Dermatol 32:403-412 (2023). PubMed: 36457234
- Rütsche D et al. Enzymatically Crosslinked Collagen as a Versatile Matrix for In Vitro and In Vivo Co-Engineering of Blood and Lymphatic Vasculature. Adv Mater 35:e2209476 (2023). PubMed: 36724374
- Li Z et al. Low-intensity pulsed ultrasound ameliorates erectile dysfunction induced by bilateral cavernous nerve injury through enhancing Schwann cell-mediated cavernous nerve regeneration. Andrology 11:1188-1202 (2023). PubMed: 36762774