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重组RabMAb

重组Anti-CD31抗体[EPR17260-254] - Low endotoxin，Azide free (ab224260)

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Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)
  • Sandwich ELISA - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)
  • Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17260-254] to CD31 - Low endotoxin, Azide free
  • Suitable for: WB, Sandwich ELISA, IP
  • Reacts with: Mouse, Rat

概述

  • 产品名称

    Anti-CD31抗体[EPR17260-254] - Low endotoxin，Azide free
    参阅全部 CD31 一抗

  • 描述

    兔单克隆抗体[EPR17260-254] to CD31 - Low endotoxin，Azide free

  • 宿主

    Rabbit

  • 特异性

    Not suitable for FC-Mouse.

  • 经测试应用

    适用于: WB, Sandwich ELISA, IPmore details
    不适用于: Flow Cyt

  • 种属反应性

    与反应: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Mouse CD31 active protein; mouse and rat platelet and lung lysates; bEnd.3 whole cell lysate. IP: bEnd.3 whole cell lysate.

  • 常规说明

    ab224260 is the carrier-free version of ab213175.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab224260于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration.

Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary.

The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31.

Ms Isoform 1-4: 69.8-81.3 kDa (predicted)

Observed band size is around 130 kDa

Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate.

Negative Control: NIH/3T3 whole cell lysate (ab7179).
Sandwich ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration.

Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary.

The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31.

Ms Isoform 1-4: 69.8-81.3 kDa (predicted)

Observed band size is around 130 kDa

Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate.

Negative Control: NIH/3T3 whole cell lysate (ab7179).
Sandwich ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
应用说明
Is unsuitable for Flow Cyt.

靶标

  • 功能

    Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

  • 组织特异性

    Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).

  • 序列相似性

    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

  • 结构域

    The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.

  • 翻译后修饰

    Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.

  • 细胞定位

    Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Adhesion molecule antibody
      • CD31 antibody
      • CD31 antigen antibody
      • CD31 EndoCAM antibody
      • EndoCAM antibody
      • FLJ34100 antibody
      • FLJ58394 antibody
      • GPIIA antibody
      • GPIIA' antibody
      • PECA1 antibody
      • PECA1_HUMAN antibody
      • Pecam 1 antibody
      • PECAM 1 CD31 EndoCAM antibody
      • PECAM antibody
      • PECAM-1 antibody
      • Pecam1 antibody
      • Platelet and endothelial cell adhesion molecule 1 antibody
      • Platelet endothelial cell adhesion molecule antibody
      • Platelet/endothelial cell adhesion molecule 1 antibody
      see all

    图片

    • Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)
      Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)

      CD31 was immunoprecipitated from 1 mg of bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate with ab213175 at 1/40 dilution.

      Western blot was performed from the immunoprecipitate using ab213175 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: bEnd.3 whole cell lysate 10µg (Input). Lane 2: ab213175 IP in bEnd.3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213175 in bEnd.3 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213175).

    • Sandwich ELISA - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)
      Sandwich ELISA - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)

      This ELISA data was generated using the same anti-CD31 antibody clone [EPR17260-254] in a different buffer formulation (cat# ab213175).

      Example of mouse CD31 standard curve in 1X Cell Extraction Buffer PTR using recombinant CD31 antibody (ab213175) and a recombinant monoclonal antibody. Background-subtracted data values (mean +/- SD) are graphed.

      For a matched antibody pair set, see Mouse CD31 Matched Antibody Pair Kit (ab212065).

      For a complete ELISA kit, see Mouse CD31 ELISA kit (ab204527).

    • Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)
      Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)

    文献 (0)

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