Anti-CD31 抗体

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

Immunohistochemical analysis of formalin fixed paraffin embedded human lung labelling CD31 with ab28364 at a concentration of 0.40 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab28364 Anti-CD31 antibody was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

ab28364 at 1/50 dillution staining CD31 in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

• IHC-P

AbReview76203****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD31 with ab28364 at 1/50 dilution (30 minutes at 23°C). 10% Neutral buffered formalin used as fixative. Heat mediated antigen retrieval was performed at 95°C for 20 minutes. Blocking performed using 2.5% serum for 20 minutes at 23°C. HRP-conjugated Goat Anti-rabbit was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Mr. Victor Bernal.

• IHC-P

AbReview76110****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

Immunohistochemistry (PFA-fixed paraffin-embedded sections) analysis of human palatine tonsil tissue labelling CD31 with ab28364 at 1/50 dilution (15 hours at 4°C). Heat mediated antigen retrieval was performed using Tris/EDTA buffer. Blocking performed with 5% normal goat serum for 1 hour at 21°C. HRP-conjugated Goat Anti-Rabbit was used as the secondary antibody.

This image is courtesy of an anonymous Abreview.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

Immunohistochemical analysis of formalin fixed paraffin embedded human lung labelling CD31 with ab28364 at a concentration of 0.50 µg/ml. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with a Bond™ Polymer Refine Detection kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.ab28364 Anti-CD31 antibody was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin. Image inset shows absence of staining in secondary antibody only control.

• IHC-P

AbReview78290****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed human skin tissue. Stained with ab28364 at 1/100 dilution. Secondary antibody used was Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG at 1/300 dilution. Blocking was done with 5% BSA for 30 minutes at 21°C. The sample was incubated with the primary antibody for 30 minutes at 4°C. Antigen retrieval method was heat mediated with citrate buffer pH 6

This image is courtesy of an anonymous Abreview