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AB53317

Anti-CD300抗体[MEM-260]

Anti-CD300 antibody [MEM-260]

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Mouse Monoclonal CD300a antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD300LF protein.

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CD300a, CMRF35H, IGSF12, HSPC083, CD300A, CMRF35-like molecule 8, CLM-8, CD300 antigen-like family member A, CMRF-35-H9, CMRF35-H, IRC1/IRC2, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, NK inhibitory receptor, CMRF35-H9, IgSF12, IRp60

2 Images
Flow Cytometry - Anti-CD300 antibody [MEM-260] (AB53317)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD300 antibody [MEM-260] (AB53317)

Separation of human monocytes (red-filled) from CD300a negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab53317 at 4 µg/ml (GAM APC).

Flow Cytometry - Anti-CD300 antibody [MEM-260] (AB53317)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD300 antibody [MEM-260] (AB53317)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab53317 at 4 µg/ml (GAM APC).

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

MEM-260

亚型

IgG1

不含载体蛋白

No

反应种属

Human

应用

Flow Cyt, WB

applications

免疫原

Cell preparation containing CD300LF protein. The exact immunogen used to generate this antibody is proprietary information.

Q8TDQ1

特异性

ab53317 recognizes CD300a, (Customer feedback suggests it also cross-reacts with CD300c, however we have not yet tested it for CD300c). The antibody MEM-260 reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.</p>" } } }

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
纯化说明
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.
存储溶液
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation (PubMed : 10746781, PubMed : 16339535, PubMed : 9701027). Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but not TRIF through activation of PTPN6 (PubMed : 22043923).
See full target information CD300A

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