重组Anti-CD30抗体[EPR4102] (ab134080)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4102] to CD30
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD30抗体[EPR4102]
参阅全部 CD30 一抗 -
描述
兔单克隆抗体[EPR4102] to CD30 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human Hodgkin's lymphoma and human tonsil tissue WB: KARPAS-299 whole cell lysate; HDLM-2 whole cell lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4102 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134080于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/200. Detects a band of approximately 120 kDa (predicted molecular weight: 64 kDa).
A 90 kDa band is precursor and 120 kDa band is the mature transmembrane form as is described in PMID: 11120787. |
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IHC-P |
1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/200. Detects a band of approximately 120 kDa (predicted molecular weight: 64 kDa). A 90 kDa band is precursor and 120 kDa band is the mature transmembrane form as is described in PMID: 11120787. |
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. -
序列相似性
Contains 6 TNFR-Cys repeats. -
翻译后修饰
Phosphorylated on serine and tyrosine residues. -
细胞定位
Cytoplasm and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 943 Human
- Omim: 153243 Human
- SwissProt: P28908 Human
- Unigene: 1314 Human
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别名
- CD 30 antibody
- CD30 antibody
- CD30 antigen antibody
see all
图片
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All lanes : Anti-CD30 antibody [EPR4102] (ab134080) at 1/200 dilution (purified)
Lane 1 : KARPAS-299 (Human anaplastic large cell lymphoma) whole cell lysates
Lane 2 : HDLM-2 (Human Hodgkin lymphoma) whole cell lysates
Lane 3 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
Lane 4 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Developed using the ECL technique.
Predicted band size: 64 kDa
Observed band size: 120,90 kDa why is the actual band size different from the predicted?
Exposure time: 60 secondsThe 90 KD band is precursor and 120 KD is the mature transmembrane form as is described in PMID: 11120787.
Raji and Daudi are negative for CD30 as is described in PMID: 29316337.
This antibody detects high background.Blocking buffer and concentration:5% NFDM/TBST
Diluting buffer and concentration:2% BSA/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [EPR4102] (ab134080)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD30 with purified ab134080 at 1/2000 dilution (0.82 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [EPR4102] (ab134080)
Immunohistochemical analysis of paraffin embedded Human Hodgkin's lymphoma tissue labelling CD30 with ab134080 (unpurified) antibody at a dilution of 1/1000. Heat mediated antigen retrieval was perfomed before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab134080 被引用在 4 文献中.
- Huo X et al. Identification of Novel Immunologic Checkpoint Gene Prognostic Markers for Ovarian Cancer. J Oncol 2022:8570882 (2022). PubMed: 36157232
- Zhang P et al. Brentuxinmab vedotin, alone or combine with bendamustine in the treatment of natural killer T cell lymphoma. Hematol Oncol 40:941-952 (2022). PubMed: 35797410
- Yeste-Velasco M et al. The potential of brentuximab vedotin, alone or in combination with current clinical therapies, in the treatment of testicular germ cell tumors. Am J Cancer Res 9:855-871 (2019). PubMed: 31218098
- Rajendran S et al. Development of a Bispecific Antibody Targeting CD30 and CD137 on Hodgkin and Reed-Sternberg Cells. Front Oncol 9:945 (2019). PubMed: 31616638