重组Anti-CD30抗体[BLR055F] - BSA free (ab272089)
![Immunocytochemistry - Anti-CD30 antibody [BLR055F] - BSA free (ab272089)](https://www.abcam.cn/ps/products/272/ab272089/Images/ab272089-3-ab252561343249anticd30antibodyimmunocytochemistrysrcellshuman.jpg "Immunocytochemistry - Anti-CD30 antibody [BLR055F] - BSA free (ab272089)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR055F] to CD30 - BSA and Azide free
- Suitable for: WB, IP, IHC-P, ICC
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD30抗体[BLR055F] - BSA free
参阅全部 CD30 一抗 -
描述
兔单克隆抗体[BLR055F] to CD30 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-P, ICCmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human CD30 aa 1-400. NP_001234.3. Extracellular domain.
Database link: P28908 -
阳性对照
- IP: MJ whole cell lysate. IHC-P: Human tonsil tissue. ICC: SR cells.
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常规说明
ab272089 is the BSA-free version of ab252561.
This product is sold under License from Bethyl Laboratories, Inc.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
无载体
是 -
Concentration information loading... -
纯化说明
Purified from cell culture supernatant. -
克隆
单克隆 -
克隆编号
BLR055F -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272089于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 64 kDa.
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| IP |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 64 kDa. |
|
IP
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC
Use at an assay dependent concentration. |
靶标
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功能
Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. -
序列相似性
Contains 6 TNFR-Cys repeats. -
翻译后修饰
Phosphorylated on serine and tyrosine residues. -
细胞定位
Cytoplasm and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 943 Human
- Omim: 153243 Human
- SwissProt: P28908 Human
- Unigene: 1314 Human
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别名
- CD 30 antibody
- CD30 antibody
- CD30 antigen antibody
see all
图片
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Immunocytochemistry - Anti-CD30 antibody [BLR055F] - BSA free (ab272089)
Formalin-fixed, paraffin-embedded sections of SR (human large cell immunoblastic lymphoma cell line) cells stained for CD30 using ab252561 at 1/250 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252561).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [BLR055F] - BSA free (ab272089)](https://www.abcam.cn/ps/products/272/ab272089/Images/ab272089-2-ab252561343250anticd30antibodyimmunohistochemistrytonsilhuman.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [BLR055F] - BSA free (ab272089)Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD30 using ab252561 at 1/250 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252561).
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![Immunoprecipitation - Anti-CD30 antibody [BLR055F] - BSA free (ab272089)](https://www.abcam.cn/ps/products/272/ab272089/Images/ab272089-1-ab252561343252anticd30antibodyimmunoprecipitationhuman.jpg)
Immunoprecipitation - Anti-CD30 antibody [BLR055F] - BSA free (ab272089)CD30 was immunoprecipitated from MJ (human cutaneous T cell lymphoma cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab252561 at 5 µL per reaction. Western blot was performed from the immunoprecipitate using ab252561 at 1/1000 dilution.
Lane 1: ab252561 IP in MJ whole cell lysate.
Lane 2: Control IgG IP in MJ whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared in NETN buffer.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252561).
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![Anti-CD30 antibody [BLR055F] - BSA free (ab272089)](https://www.abcam.cn/ps/products/272/ab272089/Images/ab272089-4-benefits-of-recombinant-antibodies.png)
Anti-CD30 antibody [BLR055F] - BSA free (ab272089)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272089 尚未被引用在任何文献中。