Anti-CD30抗体[Ber-H2]，prediluted (ab871)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (ab871)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (ab871)

Key features and details

  • Mouse monoclonal [Ber-H2] to CD30, prediluted
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-CD30抗体[Ber-H2]，prediluted
    参阅全部 CD30 一抗

  • 描述

    小鼠单克隆抗体[Ber-H2] to CD30，prediluted

  • 宿主

    Mouse

  • 特异性

    This antibody reacts with mononuclear cells in Hodgkin lymphoma, Reed-Sternberg cells and most anaplastic large cell lymphomas. It stains large cells localized around the B cell in the lymphoid tissues. This antibody stains the cell surface and the golgi region in the cells.

  • 经测试应用

    适用于: IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Tissue, cells or virus corresponding to CD30. BALB/C mice were injected with L428 cell line cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab871于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration.

This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion with proteolytic enzymes may increase staining intensity. Although not required, microwave treatment will considerably enhance the signal.
说明
IHC-P
Use at an assay dependent concentration.

This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion with proteolytic enzymes may increase staining intensity. Although not required, microwave treatment will considerably enhance the signal.

靶标

  • 功能

    Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.

  • 序列相似性

    Contains 6 TNFR-Cys repeats.

  • 翻译后修饰

    Phosphorylated on serine and tyrosine residues.

  • 细胞定位

    Cytoplasm and Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CD 30 antibody
      • CD30 antibody
      • CD30 antigen antibody
      • CD30L receptor antibody
      • Cytokine receptor CD30 antibody
      • D1S166E antibody
      • KI 1 antibody
      • KI 1 antigen antibody
      • Ki-1 antigen antibody
      • KI1 antibody
      • Lymphocyte activation antigen CD30 antibody
      • TNFRSF 8 antibody
      • Tnfrsf8 antibody
      • TNR8_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 8 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (ab871)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (ab871)
      Formalin-fixed, paraffin-embeded human Hodgkin's lymphoma tissue stained for CD30 using ab871 in immunohistochemical analysis. Antigen retrieval with EDTA buffer pH 8.0
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (ab871)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (ab871)
      IHC image of ab871 staining in Hodgkin lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab871, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    文献 (1)

    发表研究结果有使用 ab871？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab871 被引用在 1 文献中.

    • Keren L  et al. MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure. Sci Adv 5:eaax5851 (2019). Mass Cytometry . PubMed: 31633026

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